HD37 Monoclonal Antibody: A Useful Reagent for Further Characterization of “Non-T, Non-B” Lymphoid Malignancies

Pezzutto, Antonio; Dörken, Bernd; Feller, Alfred C.; Moldenhauer, Gerhard; Schwartz, Reinhard; Wernet, Peter; Thiel, Eckhard; Hünstein, W.

doi:10.1007/978-1-4612-4848-4_33

Cited by 28 publications

(12 citation statements)

References 32 publications

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“…The mAb used in this study included the anti-CD19 mAb HD37 [26], the anti-CD20 mAb 1F5 [27], the anti-human IgM p chain mAb 2C3 and (indicated here as anti-p mAb [28]), the anti-DR mAb HBlOa [28] and the anti-CD40 mAb G28-5 [29]. Antibody 187.1 is a rat anti-mouse x chain mAb [30] .These mAb were purified as described previously [28].To produce stimulatory anti-p mAb, the 2C3 mAb was conjugated to CNBr-activated Sepharose 4B beads (Pharmacia Fine Chemicals, Piscataway, NJ) at a ratio of 10 mg mAb/ml of Sepharose (anti-Ig beads; [27]).…”

Section: Antibodies and Reagentsmentioning

confidence: 99%

Signaling through CD19, Fc receptors or transforming growth factor‐β: each inhibits the activation of resting human B cells differently

et al. 1990

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To understand further the roles that negative regulatory signals may play in B cell immune responses, we compared three inhibitors of B cell proliferation: cross-linking CD19 with monoclonal antibody (mAb), signaling through Fc receptors by intact anti-mu mAb, and transforming growth factor-beta (TGF-beta). Each agent was tested for its ability to block proliferation and specific activation events induced in human tonsilar B cells activated by either cross-linking surface immunoglobulin, signaling through CD20, or direct activation of protein kinase C (PKC) by phorbol 12-myristate 13-acetate. We found that each inhibitor was functionally distinct. Both anti-CD19 mAb and anti-mu mAb inhibited anti-immunoglobulin activated cells and anti-CD20-activated cells, but neither inhibited cells activated by phorbol 12-myristate 13-acetate. TGF-beta, on the other hand, inhibited equally profoundly cells activated by each of the three regimens. These results suggest that TGF-beta blocks B cell activation at a step following the activation of PKC, whereas both signaling through CD19 and Fc receptor block early steps in the PKC activation pathway. Signaling through anti-CD19 mAb was unique in that proliferation of anti-immunoglobulin-activated cells was reduced on day 3 and then augmented subsequently. With all other inhibitory combinations the block was permanent. We conclude that each of these three inhibitors has unique important functions and therefore suggest that the effectiveness of negative signaling in B cell immune regulation will depend on the combinations of specific inhibitors modulating a specific activation program.

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Section: Antibodies and Reagentsmentioning

confidence: 99%

Signaling through CD19, Fc receptors or transforming growth factor‐β: each inhibits the activation of resting human B cells differently

et al. 1990

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“…Additionally, these cell lines had free binding capacity for u-PA. Both LCLC cell lines showed (Pezzutto et al, 1986) and was subtracted from values of MAb 98/6 binding. As positive control MAb HEA 125, an epithelium-specific marker, was used .…”

Section: X4mentioning

confidence: 99%

“…The PA system may play a role in the metastatic process of NSCLC and, in an indirect way, mesotheliomas of the lung. As control antibodies we applied MAb HEA 125 recognising an epithelium-specific surface glycoprotein of 34 kDa Momburg et al, 1987) and MAb HD37 recognising the B lymphocyte-specific differentiation antigen CD19 (Pezzutto et al, 1986).…”

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confidence: 99%

Three types of human lung tumour cell lines can be distinguished according to surface expression of endogenous urokinase and their capacity to bind exogenous urokinase

Schwartz‐Albiez

Heidtmann²,

Wolf³

et al. 1992

Br J Cancer

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Summary This study evaluates the cell surface expression of urokinase-type plasminogen activator (u-PA) and the capacity to bind exogenous urokinase as possible parameters for the distinction of various types of human lung tumours. Twelve different tumour cell lines including four small cell carcinoma, two large cell carcinoma, three squamous cell carcinoma, one adenocarcinoma and two mesothelioma cell lines of lung origin were investigated. Surface expression of endogenous u-PA was determined in a cellular radioimmunoassay (CRIA) using the u-PA-specific monoclonal antibody 98/6. To estimate additional u-PA binding capacity, exogenous two-chain, 54 kDa u-PA was employed in the CRIA. The influence of phorbol ester (PMA) treatment on expression and binding of these molecules was studied. Three different groups of lung tumour cell lines could be distinguished according to their expression of u-PA and u-PA-binding ability: (i) non small cell lung carcinoma (NSCLC) cell lines of squamous cell carcinoma/adenocarcinoma origin expressed small amounts of u-PA and bound little u-PA. Large cell carcinoma cell lines expressed high amounts of u-PA and bound large amounts of u-PA. In general, expression of u-PA and u-PA binding was enhanced after PMA treatment. (ii) Mesothelioma cell lines did not express u-PA, but were able to bind u-PA. (iii) Small cell carcinoma (SCLC) lines were devoid of surface-expressed u-PA and could not bind u-PA, both under untreated and PMA-treated conditions. It could thus be demonstrated that these three groups of lung tumour cell lines differ in their ability to express u-PA and to bind external u-PA. This may reflect the different in vivo growth behaviour and origin of the respective tumour groups.

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“…BCL1.3B3 cells were cultured in RPMI 1640 medium with 5% (vol/vol) fetal calf serum. Antibody preparations (7,8,10,11) were affinitypurified and included rabbit anti-ovalbumin (35 pg/ml), rabbit anti-BCL, idiotype (35 pg/ml), goat anti-human IgM (7.5 pg/ml), goat anti-ovalbumin (7.5 mg/ml), and anti-CD19 (HD37; 350 pg/ml).Cells were plated with antisense oligonucleotides 24 hr before the addition of antibody. Oligonucleotides were phosphorothioate derivatives synthesized by Oligos Etc.…”

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confidence: 99%

Lyn tyrosine kinase signals cell cycle arrest but not apoptosis in B-lineage lymphoma cells.

Scheuermann

Racila

Tucker

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

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Signal transduction initiated by binding of antibodis to cell surface molecules can have an important impact on the growth of tumor cells. (8), the effectiveness of anti-CD19 antibodies in preventing tumor growth probably results from a signal transduction phenomenon similar to that observed for anti-immunoglobulin (antiIg) on BCL1, rather than a classical antibody-mediated effector function, e.g., antibody-dependent cellular cytotoxicity.Here we show that anti-Ig induces an antiproliferative response in a subclone of BCL1, BCL1.3B3 (9), that includes the induction of cell cycle arrest and apoptosis. Treatment of BCL1.3B3 cells with specific antisense oligonucleotides results in the depletion of the Lyn tyrosine kinase protein.When depleted cells are challenged with anti-Ig, apoptosis is still induced, but cell cycle arrest is not. The same result is observed in anti-Ig-treated Daudi lymphoma cells. In addition, antisense lyn can prevent cell cycle arrest induced by the cross-linking ofCD19. These results indicate that the Lyn tyrosine kinase is required for transduction of the cell cycle arrest signal initiated by cross-linking of mlg or CD19. MATERIALS AND METHODSAntibodies and Antsens Olguclides. BCL1.3B3 cells were cultured in RPMI 1640 medium with 5% (vol/vol) fetal calf serum. Antibody preparations (7,8,10,11) were affinitypurified and included rabbit anti-ovalbumin (35 pg/ml), rabbit anti-BCL, idiotype (35 pg/ml), goat anti-human IgM (7.5 pg/ml), goat anti-ovalbumin (7.5 mg/ml), and anti-CD19 (HD37; 350 pg/ml).Cells were plated with antisense oligonucleotides 24 hr before the addition of antibody. Oligonucleotides were phosphorothioate derivatives synthesized by Oligos Etc. (Guilford, CT). For the BCL,.3B3-cell experiments, the sequences TG-

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HD37 Monoclonal Antibody: A Useful Reagent for Further Characterization of “Non-T, Non-B” Lymphoid Malignancies

Cited by 28 publications

References 32 publications

Signaling through CD19, Fc receptors or transforming growth factor‐β: each inhibits the activation of resting human B cells differently

Signaling through CD19, Fc receptors or transforming growth factor‐β: each inhibits the activation of resting human B cells differently

Three types of human lung tumour cell lines can be distinguished according to surface expression of endogenous urokinase and their capacity to bind exogenous urokinase

Lyn tyrosine kinase signals cell cycle arrest but not apoptosis in B-lineage lymphoma cells.

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