HCV in serum, peripheral blood mononuclear cells and lymphocyte subpopulations in C-hepatitis patients

Torres, Blanca; Martín, José Luis Ortega; Caballero, Antonio; Villalobos, Mercedes; Olea, Nicolás

doi:10.1016/s1386-6346(99)00098-4

Cited by 9 publications

(6 citation statements)

References 14 publications

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“…The reason B and T lymphocytes are not equally sensitive to HCV remains unclear. Large populations of T lymphocytes are present in the cervix under normal conditions [34]. Cytotoxic suppressor T cells are the major T cell subtype present in the cervix.…”

Section: Discussionmentioning

confidence: 99%

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Detection of Hepatitis C Virus (HCV) RNA in Normal Cervical Smears of HCV‐Seropositive Patients

et al. 2002

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The presence of hepatitis C virus (HCV) in normal cervical smears (CS) obtained from 22 HCV-seropositive and 50 HCV-seronegative patients was assessed by reverse-transcriptase-polymerase chain reaction (RT-PCR). The presence of HCV in serum was established by use of enzyme-linked immunosorbent assay, Western blot test, and RT-PCR. HCV was detected in 36.4% (n=8) of CS cells recovered from 22 HCV-seropositive patients, but not in CS samples obtained from 50 HCV-seronegative patients. Furthermore, cells from the CS of 2 seropositive/smear-positive patients and 1 seropositive/smear-negative patient were isolated; HCV RNA was detectable in the cervical lymphocytes of the 2 smear-positive patients, but not in epithelial cells or granulocytes. HCV RNA is detectable in the CS of some HCV-seropositive women. The clinical importance of these data requires further study.

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Section: Discussionmentioning

confidence: 99%

“…Numerous authors have reported that, for detection of HCV RNA, RT-PCR of WPB samples was significantly more sensitive than RT-PCR of plasma samples [21,34]. As a result, these authors recommended the determination of HCV content via PCR technique in the serum as well as in the blood cells of each patient.…”

Section: Discussionmentioning

confidence: 99%

Detection of Hepatitis C Virus (HCV) RNA in Normal Cervical Smears of HCV‐Seropositive Patients

et al. 2002

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“…DCs can be generated from peripheral blood monocytes ex vivo , loaded with relevant peptides, DNA or mRNA, and subsequently readministered to the same patient where they will prime naïve T cells. However, the presence of HCV has been demonstrated in all major peripheral blood mononuclear cell (PBMC) subpopulations of chronic hepatitis C patients: B cells (21–27), CD4 + T cells (21, 23, 24), CD8 + T cells (21–24, 26), monocytes (21–23), and DCs (10, 25–27). Furthermore, monocyte‐derived DCs can be infected in vitro (28).…”

mentioning

confidence: 99%

Monocyte‐derived dendritic cells from chronic HCV patients are not infected but show an immature phenotype and aberrant cytokine profile

Gelderblom

Nijhuis

Jong

et al. 2007

Liver International

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“…A shortcoming of our procedure is that the volume of blood available for testing did not yield sufficient platelets to test platelet concentrates. This is unfortunate since previous publications have shown that platelets presented specific ligands to HCV that may be able to concentrate viral particles [13, 23–26].…”

Section: Discussionmentioning

confidence: 99%

Distribution of hepatitis C virus in circulating blood components from blood donors

Chancey¹,

Winkelman²,

Foley³

et al. 2012

Vox Sanguinis

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Background and Objectives Current nucleic acid tests (NAT) for blood donor screening use plasma as the test sample and, consequently, cannot detect virions bound to blood cells of infected donors. Hepatitis C virus (HCV) RNA and infectious virions have been detected in association with the cellular components of blood of patients with active liver disease; however, studies comparing HCV viral loads in whole blood and plasma have generated contradictory results. The aim of this study was to investigate the distribution of HCV in different compartments of the peripheral blood from HCV‐infected blood donors, which may differ from that observed in patients with HCV‐associated liver disease. Materials and Methods Hepatitis C virus‐positive donor specimens were identified by NAT and antibody testing. HCV RNA was extracted from samples of whole blood and their corresponding components (RBC and plasma). Viral RNA was quantified by real‐time qRT‐PCR. Results Hepatitis C virus was present in all blood components from infected donors from which RNA could be amplified. For the majority of samples, plasma (34/46) had the highest detectable concentration of HCV RNA, and RBC (37/46) had the lowest. Specimens with negative NAT and positive antibody assays also produced qRT‐PCR negative results. Conclusion These results indicate that including the RBC fraction in the tested sample will not increase assay sensitivity. Although 10% of the specimens had a higher viral load in whole blood, there was no significant overall increase in sensitivity to justify changes in the specimen format. Thus, plasma specimens are well suited for blood donor screening for HCV.

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HCV in serum, peripheral blood mononuclear cells and lymphocyte subpopulations in C-hepatitis patients

Cited by 9 publications

References 14 publications

Detection of Hepatitis C Virus (HCV) RNA in Normal Cervical Smears of HCV‐Seropositive Patients

Detection of Hepatitis C Virus (HCV) RNA in Normal Cervical Smears of HCV‐Seropositive Patients

Monocyte‐derived dendritic cells from chronic HCV patients are not infected but show an immature phenotype and aberrant cytokine profile

Distribution of hepatitis C virus in circulating blood components from blood donors

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