HCR/DNAzyme-triggered cascaded feedback cycle amplification for self-powered dual-photoelectrode detection of femtomolar HPV16

Recent Advances in Photoelectrochemical Sensing for Food Safety

“…The key to this technique lies in the design of a series of nucleic acid molecular reactions to amplify the initial detection signal, including nucleic acid hybridization chain reaction (HCR), rolled-circle amplification (RCA), signal-amplifying DNA probes, and isothermal amplification technology (LAMP), etc. Researchers innovatively developed a dual photoelectrode system that used Zn-TBAPy pyrene-based MOF as the photocathode and BiVO 4 /Ti 3 C 2 Schottky structure as the photoanode . When the target HPV16 is added, the HCR reaction is triggered, generating DNAzyme to cut DNA (H7) and produce H7–1 and H7–2.…”

Exonuclease-III assisted the target recycling coupling with hybridization chain reaction for sensitive mecA gene analysis by using PGM

“…Hybridization chain reaction (HCR) is an initiator-or target-triggered toehold-mediated strand displacement reaction (9,26,5). It has been used as an enzyme-free signal ampli cation approach for nucleic acid detection because of its simple protocols and high ampli cation e ciency.…”

Target Recognition Triggered Split DNAzyme based Colorimetric Assay for Direct and Sensitive Methicillin-Resistance Analysis of Staphylococcus aureus

“…Nucleic acid-based strategies offer greater advantages compared to other methods due to their ability to identify antibiotic resistance genes, such as the mecA gene [11]. In general, polymerase chain reaction (PCR) and other isothermal amplification technologies, such as loop-mediated isothermal amplification and nucleic acid sequence-based amplification, have sufficient sensitivity [12][13][14]. However, the PCR-based method requires advanced equipment and accurate temperature cycling, whereas isothermal amplification methods are not available to detect a single nucleotide.…”