hCG, Hyperglycosylated hCG, Pituitary hCG, Cancer hCG and Fetal hCG

Development of Immobilized Enzyme Reactors for the characterization of the glycosylation heterogeneity of a protein

“…After the evaluation of the repeatability of the digestion on T-IMER in terms of number of glycans identified on a specific location, the performance of T-IMER digestion was compared to conventional in-solution digestion using the same sample of hCG to confirm the benefit of IMERs. As the digestions on IMERs and in solution were carried out with 2.5 µg of hCG, by considering an average molecular mass of 35 kDa [24] and taking into account the grafting yield, the E/S ratio was evaluated up to 1,430 for T-IMER. This E/S ratio was roughly 150,000 fold higher than the one of digestion in solution (1:100), thus explaining the short digestion time used with IMERs (30 min).…”

“…Carbohydrate moieties represent ± 30% of its molecular weight. hCG is a heterodimer of the α subunit (hCGα) and the β subunit (hCGβ) with two Nglycosylation sites on each subunit (respectively noted α N 52 , α N 78 , β N 13 , and β N 30 in order of appearance in the amino acid sequence) and four O-glycosylation sites on hCGβ [23,24].…”