HbWRKY27, a group IIe WRKY transcription factor, positively regulates HbFPS1 expression in Hevea brasiliensis

Abstract

Farnesyl pyrophosphate synthase (FPS) is a key enzyme that catalyzes the formation of farnesyl pyrophosphate, the main initiator for rubber chain initiation in Hevea brasiliensis Muell. Arg. The transcriptional regulatory mechanisms of the FPS gene still not well understood. Here, a WRKY transcription factor designated HbWRKY27 was obtained by screening the latex cDNA library applied the HbFPS1 promoter as bait. HbWRKY27 interacted with the HbFPS1 promoter was further identified by individual Y1H and EMSA assays. HbWRKY27 belongs to group IIe WRKY subfamily which contains a typical WRKY domain and C-X5-CX23-HXH motif. HbWRKY27 was localized to the nucleus. HbWRKY27 predominantly accumulated in latex. HbWRKY27 was up-regulated in latex by ethrel, salicylic acid, abscisic acid, and methyl jasmonate treatment. Transient expression of HbWRKY27 led to increasing the activity of the HbFPS1 promoter in tobacco plant, suggesting that HbWRKY27 positively regulates the HbFPS1 expression. Taken together, an upstream transcription factor of the key natural rubber biosynthesis gene HbFPS1 was identified and this study will provide novel transcriptional regulatory mechanisms of the FPS gene in Hevea brasiliensis.

Differential Expression of lncRNAs and miRNAs Between Self-Rooting Juvenile and Donor Clones Unveils Novel Insight Into the Molecular Regulation of Rubber Biosynthesis in Hevea brasiliensis

“…HbCZF1 positively regulates the expression of hmg1 , which encodes HMGR ( Guo et al, 2015 ). HblMYB19, HblMYB44, and HbWRKY27 positively regulate the expression of the farnesyl pyrophosphate synthase (FPS) gene HbFPS1 ( Wang et al, 2017 ; Qu et al, 2020 ). HMGR and FPS are key enzymes during natural rubber biosynthesis.…”

“…The reactive oxygen species (ROS) production and ROS-scavenging in laticifers affect latex coagulation ( Zhang et al, 2019b ). In addition, transcription factors are actively implicated in the regulation of latex regeneration ( Wang et al, 2013b ; Li et al, 2016b , 2020a ; Zhang et al, 2019b ; Qu et al, 2020 ). In the present study, DELs acting in the cis-regulation were found.…”

“…Furthermore, XR_002490996.1 was spaced 9.93 kb downstream of XM_021783538.1, which encodes a MADS-box transcription factor, and TCONS_00023828 was located 0.45 kb upstream of XM_021814952.1, which encodes a WRKY protein. The MADS-box and WRKY transcription factor have been identified to negatively regulate the biosynthesis of natural rubber ( Wang et al, 2013b ; Li et al, 2016b , 2020b ; Qu et al, 2020 ). XM_021783538.1 and XM_021814952.1 were down-regulated in self-rooting JCs ( Figures 5D , E ).…”

Systematic evolution of bZIP transcription factors in Malvales and functional exploration of AsbZIP14 and AsbZIP41 in Aquilaria sinensis

“…Agrobacterium tumefaciens GV3101 strains harboring pAsbZIP14-GFP, pAsbZIP41-GFP, or pNC-Cam1304-SubC plasmids were separately grown to OD600 = 0.8 in LB medium at 200 rpm and 28°C and infiltrated into the onion epidermis by Agrobacterium -mediated transformation. The transformed onion epidermis was incubated in darkness at 27°C for 70 h. The GFP fluorescent signal of these onion epidermal cells was monitored using a Leica confocal fluorescence microscope (Wetzlar, Germany) after the nuclei were stained with 20 μg/mL 4´, 6-diamidino-2- phenylindole (DAPI) for 5 min and washed three times with the saline ( Qu et al., 2020 ; Li et al., 2021a ).…”

HbTGA1, a TGA Transcription Factor From Hevea brasiliensis, Regulates the Expression of Multiple Natural Rubber Biosynthesis Genes

“…HbCZF1 upregulates the expression of hmg1 ( Guo et al, 2015 ). HblMYB19, HblMYB44, and HbWRKY27 upregulated the expression of HbFPS1 ( Wang et al, 2017 ; Qu et al, 2020 ). HbRZFP1 downregulated HRT2 expression ( Guo et al, 2018 ).…”