HBV-DNA as marker of hepatitis B virus infection in autochthonous and foreign population of the Molecular Biology Laboratory of the Immuno-Haematology and Blood Transfusion Unit, AO “S. Maria”, Terni

Cappuccini, Daniela; Grespi, Valentina; Proietti, Monica; Pagnani, Alessandra; Medori, Maria Chiara; Scaccetti, Augusto

doi:10.4081/mm.2014.4429

Cited by 1 publication

(2 citation statements)

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“…Italy, land of immigration, has become a multi-ethnic country with people coming from high and medium endemic disease areas in terms of HBV (5). Chronic hepatitis is one of the most widespread infections among immigrants, and it is mainly caused by HBV (13,14).…”

Section: Discussionmentioning

confidence: 99%

“…The assessment of the HBV-DNA was performed on plasma, obtained through the centrifugation (3000 rpm for 20 minutes) of whole blood, taken and transferred into sterile test tubes containing ethylenediaminetetraacetic acid (5). The quantification of HBV-DNA in specimens was carried out through COBAS AmpliPrep/COBAS TaqMan HBV v2.0 Roche test, which enables the automated extraction and amplification with subsequent detection of the virus nucleic acid (measuring range between 20 and 1.7 E+08 IU/mL) (5).…”

Section: Methodsmentioning

confidence: 99%

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Genotypes of the hepatitis B virus within the area of Terni, Italy: our experience

et al. 2016

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SummaryBackground and aims: The presence of hepatitis B varies depending on the different areas of the world; 10 genotypes of hepatitis B virus (HBV) (A to J) have been identified, and they differ from one another in the nucleotide sequence and geographical location. The various genotypes are associated with a different evolution of the disease and with distinct responses to treatment.Materials and Methods: From January 2010 to March 2014 we assessed the genotype of the HBV virus on 35 specimens with HBV-DNA>1000 IU/mL. The HBV genotype has been determined through sequencing.Results: The 35 specimens belonged to individuals with a mean and median age of 42.8 and 40 years respectively: 17 of them were Italian and 18 from other countries. In total there were 19 males: 12 Italians and 7 foreigners. Females were 16: 5 Italians and 11 foreigners. The subjects with HBV-DNA≥10 6 IU/mL were prevailing, followed by subjects with HBV-DNA between 1000 IU/mL and 10.000 IU/mL. Out of 35 patients analysed by genotype, we found 20 genotypes D and 15 non-D genotypes. Conclusions:The analyses carried out on results suggest that Italy, land of immigration, has become a multi-ethnic country with people coming from high and medium endemic disease areas in terms of HBV. Most patients show D genotype, however the migratory flows lead to the introduction of patients with non-D HBV genotypes in the native population as highlighted in Terni.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%