HbMADS4, a MADS-box Transcription Factor from Hevea brasiliensis, Negatively Regulates HbSRPP

Li, Hui‐Liang; Wei, Li-Ran; Guo, Dong; Wang, Ying; Zhu, Jiahong; Xiong-ting, Chen; Peng, Shi-Qing

doi:10.3389/fpls.2016.01709

Cited by 32 publications

(29 citation statements)

References 45 publications

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“…Other WRKY proteins may participate in the regulation of NR biosynthesis ( Li et al, 2014 ). HbMADS4 was also found as a negative transcriptional regulator of HbSRPP ( Li et al, 2016b ). HbCZF1, a CCCH-type zinc-finger protein, highly activates the hmg1 promoter, and HbCZF1 may help regulate NR biosynthesis ( Guo et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

“…However, the biological functions of the rubber molecule and latex remain unclear ( Ko et al, 2003 ). The regulatory mechanisms of NR biosynthesis are also poorly understood ( Li et al, 2016b ; Tang et al, 2016 ; Yamashita et al, 2016 ). MYB TFs play vital roles in regulating plant secondary metabolic pathways, such as the general phenylpropanoid pathway and lignin, flavonoid, and glucosinolate pathways ( Chezem et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome-Wide Identification and Characterization of MYB Transcription Factor Genes in the Laticifer Cells of Hevea brasiliensis

Wang

Zhan

et al. 2017

Front. Plant Sci.

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MYB transcription factors hold vital roles in the regulation of plant secondary metabolic pathways. Laticifers in rubber trees (Hevea brasiliensis) are of primary importance in natural rubber production because natural rubber is formed and stored within these structures. To understand the role of MYB transcription factors in the specialized cells, we identified 44 MYB genes (named HblMYB1 to HblMYB44) by using our previously obtained transcriptome database of rubber tree laticifer cells and the public rubber tree genome database. Expression profiles showed that five MYB genes were highly expressed in the laticifers. HblMYB19 and HblMYB44 were selected for further study. HblMYB19 and HblMYB44 bound the promoters of HbFDPS1, HbSRPP, and HRT1 in yeast. Furthermore, the transient overexpression of HblMYB19 and HblMYB44 in tobacco plants significantly increased the activity of the promoters of HbFDPS1, HbSRPP, and HRT1. Basing on this information, we proposed that HblMYB19 and HblMYB44 are the regulators of HbFDPS1, HbSRPP, and HRT1, which are involved in the biosynthesis pathway of natural rubber.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptome-Wide Identification and Characterization of MYB Transcription Factor Genes in the Laticifer Cells of Hevea brasiliensis

Wang

Zhan

et al. 2017

Front. Plant Sci.

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“…Yeast one-hybrid assay was performed according to the method described by Li et al [ 20 ]. The bait vector pAbAi-MeAGPS1a was constructed for yeast one-hybrid assay, and the cassava storage root total RNA was isolated.…”

Section: Methodsmentioning

confidence: 99%

MePHD1 as a PHD-Finger Protein Negatively Regulates ADP-Glucose Pyrophosphorylase Small Subunit1a Gene in Cassava

Chen

Liu

et al. 2018

IJMS

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ADP-glucose pyrophosphorylase (AGPase) is an important enzyme in the starch synthesis pathway. Its enzyme activity can determine the efficiency of starch biosynthesis. Cassava (Manihot esculenta Crantz) is the main staple crop worldwide and has a high starch content in its storage root. However, the inner regulatory mechanism of AGPase gene family is unclear. MePHD1; a plant homeodomain transcription factor; was isolated through a yeast one-hybrid screening using the promoter of ADP-glucose pyrophosphorylase small subunit1a (MeAGPS1a) as bait, and cassava storage root cDNA library as prey. This factor could bind to the MeAGPS1a promoter in vitro and in vivo, and its predicted binding region ranged from −400 bp to −201 bp, at the translation initiation site. The transcript level of MePHD1 could be induced by five plant hormones, and a temperature of 42 °C. This was down-regulated during the maturation process of the storage root. MePHD1 protein could repress the promoter activity of MeAGPS1a gene by a dual-luciferase assay; which indicated that MePHD1 is a negative regulator for the transcript level of MeAGPS1a gene.

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“…A MADS‐box TF gene ( HbMADS4, inducible by methyl jasmonate (MeJA) and ethylene, and preferentially expressed in latex) was recently isolated from H. brasiliensis . A yeast one‐hybrid experiment, which used the HbSRPP promoter as bait, demonstrated that overexpression of HbMADS4 suppressed HbSRPP promoter activity in transgenic N. tabacum plants (Li et al ., ). Similarly, GUS activity was suppressed when pHbSRPP::GUS was co‐expressed with CaMV35S::HbWRKY1 , in N. tabacum , making it likely that the transcription factor, WRKY, behaves as a negative regulator of HbSRPP in NR biosynthesis (Wang et al ., , ).…”

Section: Proteins and Genes Involved In Nr Substrate Biosynthesismentioning

confidence: 97%

Natural rubber biosynthesis in plants, the rubber transferase complex, and metabolic engineering progress and prospects

Cherian

Ryu

Cornish

2019

Plant Biotechnology Journal

121

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Summary Natural rubber (NR) is a nonfungible and valuable biopolymer, used to manufacture ~50 000 rubber products, including tires and medical gloves. Current production of NR is derived entirely from the para rubber tree (Hevea brasiliensis). The increasing demand for NR, coupled with limitations and vulnerability of H. brasiliensis production systems, has induced increasing interest among scientists and companies in potential alternative NR crops. Genetic/metabolic pathway engineering approaches, to generate NR‐enriched genotypes of alternative NR plants, are of great importance. However, although our knowledge of rubber biochemistry has significantly advanced, our current understanding of NR biosynthesis, the biosynthetic machinery and the molecular mechanisms involved remains incomplete. Two spatially separated metabolic pathways provide precursors for NR biosynthesis in plants and their genes and enzymes/complexes are quite well understood. In contrast, understanding of the proteins and genes involved in the final step(s)—the synthesis of the high molecular weight rubber polymer itself—is only now beginning to emerge. In this review, we provide a critical evaluation of recent research developments in NR biosynthesis, in vitro reconstitution, and the genetic and metabolic pathway engineering advances intended to improve NR content in plants, including H. brasiliensis, two other prospective alternative rubber crops, namely the rubber dandelion and guayule, and model species, such as lettuce. We describe a new model of the rubber transferase complex, which integrates these developments. In addition, we highlight the current challenges in NR biosynthesis research and future perspectives on metabolic pathway engineering of NR to speed alternative rubber crop commercial development.

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HbMADS4, a MADS-box Transcription Factor from Hevea brasiliensis, Negatively Regulates HbSRPP

Cited by 32 publications

References 45 publications

Transcriptome-Wide Identification and Characterization of MYB Transcription Factor Genes in the Laticifer Cells of Hevea brasiliensis

Transcriptome-Wide Identification and Characterization of MYB Transcription Factor Genes in the Laticifer Cells of Hevea brasiliensis

MePHD1 as a PHD-Finger Protein Negatively Regulates ADP-Glucose Pyrophosphorylase Small Subunit1a Gene in Cassava

Natural rubber biosynthesis in plants, the rubber transferase complex, and metabolic engineering progress and prospects

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