HBLV (orHHV-6) in human cell lines

Ablashi, Dharam V.; Salahuddin, S. Z.; Josephs, S.F.; Imam, Farhad; Lusso, Paolo; Gallo, Robert C.; Hung, Chia Chun; Lemp, John F.; Pd, Markham

doi:10.1038/329207a0

Cited by 176 publications

(67 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…One particular aspect of HHV-6 pathogenesis is its ability to infect cell types of hematopoietic origin. HHV-6 can infect T cells (7), B cells (1), NK cells (8), megakaryocytes (9), and monocytes/macrophages (10,11). It is also suggested that HHV-6 can establish a latent infection in the monocyte/macrophage lineage (10).…”

mentioning

confidence: 99%

Activation of Monocyte Cyclooxygenase-2 Gene Expression by Human Herpesvirus 6

Janelle¹,

Gravel²,

Gosselin³

et al. 2002

Journal of Biological Chemistry

View full text Add to dashboard Cite

Prostaglandin E 2 (PGE 2 ) is an arachidonic acid metabolite mainly produced by activated monocytes/macrophages (Mo/M) that display broad immunomodulatory activities. Several viruses capable of infecting Mo/M modulate PGE 2 synthesis in a way that favors the infection processes and the spread of virions. In the present work, we studied the effect of human herpesvirus 6 (HHV-6) infection of Mo/M on PGE 2 synthesis. Our results indicate that HHV-6 induces COX-2 gene expression and PGE 2 synthesis within a few hours of infection. We mapped the different promoter elements associated with COX-2 gene activation by HHV-6 to two cis-acting elements: a cyclic AMP-responsive element and an activator protein-1 element. HHV-6 immediate-early protein 2 was identified as a modulator of COX-2 gene expression in Mo/M. Finally, addition of PGE 2 to HHV-6-infected peripheral blood mononuclear cells cultures was found to increase significantly viral replication. Overall, these results further contribute to the immunomodulatory properties of HHV-6 and highlight a potential role for eicosanoids in the replication process of this virus.

show abstract

mentioning

confidence: 99%

Activation of Monocyte Cyclooxygenase-2 Gene Expression by Human Herpesvirus 6

Janelle¹,

Gravel²,

Gosselin³

et al. 2002

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…HHV-6 could be isolated mainly from T lymphocytes of patients with various diseases. Although it was reported that HHV-6 could replicate in B lymphocytes, it was corrected to the notion that this virus propagates dominantly in CD4+ lymphocytes (1,3,22,56,85). However, CD4 molecule is not the receptor for HHV-6 (57), and T-cell activation is required for efficient replication of HHV-6 (29, 43).…”

Section: Latent Infection and The Reactivation Of Hhv-6 (mentioning

confidence: 99%

“…However, CD4 molecule is not the receptor for HHV-6 (57), and T-cell activation is required for efficient replication of HHV-6 (29, 43). Furthermore, HHV-6 infects a variety of human cells such as T and B lymphocytes of peripheral blood, cell lines of lymphocyte and macrophage in vitro (1,3,22,85), and infects also lymphocytes of chimpanzee in vitro (60). We also tried to isolate the virus from peripheral blood of ES patients (84).…”

Section: Latent Infection and The Reactivation Of Hhv-6 (mentioning

confidence: 99%

Human Herpesvirus 6

Yamanishi

1992

Microbiology and Immunology

View full text Add to dashboard Cite

551 552K. YAMANISHI balloon-like cells could be observed. When the cells were fixed and stained with the sera of acute and convalescent phase of ES, positive staining was observed only with the convalescent sera. Next, the cultured cells were observed by electron microscopy (EM) as reported elsewhere (74). The typical herpesvirus particles were found in the nucleus and cytoplasm of the cultured cells. Thus, the diameter of virion was 90-110 nm in the nucleus and 150-170 nm in the cytoplasm (91, 95), suggesting that this virus resembles a herpesvirus. Next, sera, which were specific for 5 human pathogenic herpesviruses described above, were tested for the reactivity to this viral antigen in the cultured cells, and no positive staining was observed. These results suggested that a new herpesvirus was isolated from ES patients. Then this viral antigen and HHV-6 antigen (55) were reacted with paired sera from ES patients. No antibody to both antigens was detected in the sera during the acute phase and the antibody titers to both antigens increased during the convalescent phase. This result indicated that the virus isolated from ES patients was HHV-6. Thereafter this result was confirmed by other groups, and the virus isolation rate from ES patients was shown to be high at the febrile phase of ES (6, 13). Furthermore, it was reported that HHV-6 infection without clinical symptoms of rash or fever, or even without any clinical symptoms could be seen in some individuals as the primary infection of 81,83).3. Other diseases associated with HHV-6 infection. Although the symptoms of ES are relatively mild, liver dysfunction and neurological symptoms such as convulsion are seen during ES (62). Recently it has been reported that HHV-6 caused the liver dysfunction in the patients who were in the primary infection of HHV-6 (9, 82, 88), and that unfortunately one patient died from fulminant hepatitis (9).A case that was diagnosed as encephalitis during HHV-6 infection was also reported (36). In order to detect HHV-6 in the central nervous system, we tried to detect HHV-6 DNA in the cerebrospinal fluid (CSF) from ES patients (93). CSF was obtained from patients showing the neurological symptoms, such as convulsion, vomiting, irritability and bulging of anterior fontanel, during febrile phase of ES. Since patients had high fever (more than 39 C) and the appearance of rash after subsidence of the fever, they were clinically diagnosed as ES. When cell counts in CSF were performed at the time of sample collection, they ranged in normal in each sample. CSF samples as controls were collected from patients who were admitted in the hospital and suspected as having neurological symptoms or lasting high fever by unknown causes. Sixteen specimens including 6 control samples of CSF were collected from the patients, and DNA was extracted and amplified by PCR using HHV-6 specific primers. The band specific for HHV-6 DNA could be detected by ethidium bromide staining in 5 of the 10 ES patients, CSF (50%), and this was confirmed by Southern blot...

show abstract

“…Certain strains of HHV-6 grow well in certain cell lines and significant amounts of cell-free virus have been produced (Ablashi et al, 1987;Okuno et al, 1989;Shiraki et al, 1989a, b). The stability of infectivity is much influenced by the particular preparation and buffers used to contain celt-free virus (Asano & Takahashi, 1978;Farnham & Newton, 1959;Vonka & BenyeshMelnick, 1966;Wentworth & Gloyd, 1968;Hoggan & Roizman, 1959) and therefore the virus particle/infectivity ratio of HHV-6 will be much improved when appropriate conditions for the preparation of cell-free virus are achieved.…”

Section: Effect Of Ph On Hhv-6 Infectivitymentioning

confidence: 99%