‘Hbe minus’ mutants of hepatitis B virus. Molecular characterization and its relation to viral genotypes

López, José Luis; Mbayed, Viviana Andrea; Telenta, P.F.S.; González, Jorge; Campos, Rodolfo Héctor

doi:10.1016/s0168-1702(02)00078-3

Cited by 15 publications

(9 citation statements)

References 22 publications

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“…Eighty-seven percent of tested anti-HBe positive patients showed PC and/or CP mutants. Unlike a previous study [Rodriguez-Frias et al, 1995], and in accordance with Lopez et al [2002], we showed the presence of a G 1896 A stop codon PC mutation associated with CP mutations in HBV/A infection. This suggests that the G 1896 A stop codon mutation occurs even in HBV/A infected patients, at least in some cases, despite the presence of a C at position 1858 pairing with the G 1896.…”

Section: Discussionsupporting

confidence: 84%

Significance of hepatitis B virus genotypes A to E in a cohort of patients with chronic hepatitis B in the Seine Saint Denis District of Paris (France)

et al. 2006

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The aim of this study was to examine the genetic variability of the hepatitis B virus (HBV) and its significance. HBV genotypes, core promoter and precore mutants were characterized in 109 consecutive patients with biopsy-proven HBV chronic hepatitis. Genotypes A (26.6%), B (12.8%), C (18.3%), D (18.3%), and E (14.7%) indicate a wide genotypic distribution. Patients were from Asia (30.3%), Europe (28.4%), Sub Saharan Africa (23.9%), the Caribbean (10.1%), North Africa (5.5%), and Madagascar (1.8%). HBV genotypes A and D (HBV/A and /D) infected all subgroups except Asian patients. HBV/B or /C were found in 97% of Asian patients, whereas HBV/E only infected sub-Saharan African and Caribbean patients. Differences according to genotypes were: an increased prevalence of anti-HBe antibodies in patients infected with HBV/D (P = 0.003), higher serum transaminases in patients infected with HBV/A and/D (P = 0.043), more severe liver fibrosis in patients infected with HBV/A, /C and/D (P = 0.02). Precore and core promoter mutants were found in 87% of anti-HBe positive patients, and were associated with HBV/D (P = 0.04) and severe liver fibrosis (P = 0.002). It is concluded that HBV genotypes A, B, C, D, and E circulate in the Seine Saint Denis District, reflecting the geographical origin of patients. HBV/A, /C and/D seem to be associated with more severe hepatic disease.

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Section: Discussionsupporting

confidence: 84%

Significance of hepatitis B virus genotypes A to E in a cohort of patients with chronic hepatitis B in the Seine Saint Denis District of Paris (France)

et al. 2006

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“…Precore mutant HBV strains have long been detected in chronically infected Argentinean patients (38,39). In the present work, mutations in the precore region and in the core promoter were found to be very frequent among blood donors.…”

Section: Discussionsupporting

confidence: 48%

“…The presence of this mutation was also confirmed in three other samples (4.0%) which contained both wild-type codon 28 and mutant codon 28. Similarly, López et al (39) stop codon 28 mutation completely abolishes HBeAg synthesis (7). Although HBeAg is not required for viral replication, HBV strains unable to express HBeAg can be considered slow replicative variants.…”

Section: Discussionmentioning

confidence: 97%

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Strong Association between Genotype F and Hepatitis B Virus (HBV) e Antigen-Negative Variants among HBV-Infected Argentinean Blood Donors

et al. 2004

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A number of reports have indicated an increased risk of cirrhosis and hepatocellular carcinoma in hepatitis B virus (HBV)-infected individuals carrying HBV e antigen (HBeAg)-negative variants. Although distinct core promoter and precore mutations distributed according to geographical locality and viral genotype have been reported, epidemiological data from South America are still scarce. The prevalences of HBV genotypes and core promoter and precore polymorphisms in 75 HBeAg-negative Argentinean blood donors were surveyed. The observed frequencies of HBV genotypes were 64.0% for genotype F, 17.3% each for genotypes A and D, and 1.3% for genotype C. Genotype F strains were widely distributed and significantly more prevalent in the northern region of the country (P < 0.001). An overall high proportion of a stop codon mutation (UAG) at precore codon 28 (66.7%) was observed. Wild-type codon 28 (UGG) was present in 29.3% of the samples, and the remaining 4.0% of samples had mixed variants. The combination of A at nucleotide (nt) 1762 and G at nt 1764 of the core promoter was found in 58.7% of the samples. The variant profiles-T at nt 1762 and A at nt 1764 or A at nt 1762 and A at nt 1764-were detected in 28.0 and 1.3% of the samples, respectively. The observed core promoter polymorphisms could not be related to the ratio of HBeAg to anti-HBeAg antibody, HBV genotype, or precore codon 28 status. Nevertheless, a clear association of genotype F and a precore stop codon mutation was found (P < 0.05). In conclusion, HBV genotype F and mutant codon 28 strains predominated and were strongly associated in a geographically broad Argentinean blood donor population.

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“…Studies regarding the prevalence of HBV genotypes have been performed in many regions of the World, including Asia [Alestig et al, 2001;Ding et al, 2001Ding et al, , 2002Orito et al, 2001;Chan et al, 2002;Cui et al, 2002;Liu et al, 2002;Ogawa et al, 2002;Sakugawa et al, 2002;Thakur et al, 2002;Kato et al, 2002b;Gandhe et al, 2003;Lee et al, 2003;Lusida et al, 2003;Sallam and William Tong, 2004], America [Arauz-Ruiz et al, 1997;Blitz et al, 1998;Mbayed et al, 1998;Moraes et al, 1999;Nakano et al, 2001;Arauz-Ruiz et al, 2002;López et al, 2002;Quintero et al, 2002;Sá nchez et al, 2002;Chu et al, 2003], Africa [Borchani-Chabchoub et al, 2000;Sugauchi et al, 2003;Suzuki et al, 2003], and Australia [Alestig et al, 2001;Sugauchi et al, 2001]. Specific data coming from Europe [Rodríguez-Frías et al, 1995;Grandjacques et al, 2000;Sá nchez-Tapias et al, 2002;Abe et al, 2004;Echevarría and León, 2004;Koppelman and Zaaijer, 2004;Krekulova et al, 2004;Tallo et al, 2004] are, however, limited.…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of hepatitis B virus in Spain: Identification of viral genotypes and prediction of antigenic subtypes by limited sequencing

Echevarría

Avellón

Magnius

2005

Journal of Medical Virology

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The hepatitis B virus (HBV) genotypes were studied by a line probe assay (LiPA) and by direct sequencing of a 339 nucleotide fragment from the S region of the viral genome in samples from 269 carriers living in Spain, either native to Spain (231) or immigrants from Africa, Asia, and Eastern Europe (38). The sequences were also used to predict the HBV surface antigen (HBsAg) subtype on the basis of the amino acids specified at selected positions of the HBsAg molecule. Agreement between the two genotyping methods was found in most cases (98.1%) and a HBV genotype could be assigned to all samples. The viral groups D/ayw2 (30.1%), D/ayw3 (28.6%), and A/adw2 (21.2%) were prevalent, with an additional participation of the groups D/ayw4 (4.8%), F/adw4q- (1.9%), A/ayw1 (1.9%), and D/adw3 (0.7%), all of them present among the autochthonous carriers. Strains from genotypes B and C were found exclusively among Chinese immigrants. Genotype E strains were found in immigrants from Central Africa and in one patient native of Spain. Point mutations leading to amino acid changes of residues involved in the expression of the HBsAg subtype determinants were found in 12 samples (4.5%). Some mutations would predict the putative novel genotype-subtype associations A/adw4q+, A/ayr, D/ayr, and E/ayw1, while others would suggest the loss of subtype-specific determinants. The finding of HBV strains characteristic for Africa among the autochthonous carriers confirms the emergence of African HBV strains in Spain.

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‘Hbe minus’ mutants of hepatitis B virus. Molecular characterization and its relation to viral genotypes

Cited by 15 publications

References 22 publications

Significance of hepatitis B virus genotypes A to E in a cohort of patients with chronic hepatitis B in the Seine Saint Denis District of Paris (France)

Significance of hepatitis B virus genotypes A to E in a cohort of patients with chronic hepatitis B in the Seine Saint Denis District of Paris (France)

Strong Association between Genotype F and Hepatitis B Virus (HBV) e Antigen-Negative Variants among HBV-Infected Argentinean Blood Donors

Molecular epidemiology of hepatitis B virus in Spain: Identification of viral genotypes and prediction of antigenic subtypes by limited sequencing

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