HB-EGF embedded in PGA/PLLA scaffolds via subcritical CO 2 augments the production of tissue engineered intestine

Nelson, Tyler; Cromeens, Barrett P.; Rager, Terrence M.; Lannutti, John J.; Johnson, Jed; Besner, Gail E.

doi:10.1016/j.biomaterials.2016.06.039

Cited by 27 publications

(27 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We anticipated that the immobilized bFGF should be more active to boost growth of NIH‐3T3 cells due to its expanded duration of effect. As highlighted in majority of recent studies, the immobilized growth factors have been confirmed to be positive in cell functions and tissue regeneration both in vitro and vivo environments . However, only a few researches explored the stimulation of cells by growth factors which could be immobilized on surfaces of biomaterials at certain densities of growth factors.…”

Section: Resultsmentioning

confidence: 99%

Binding efficiency of recombinant collagen‐binding basic fibroblast growth factors (CBD‐bFGFs) and their promotion for NIH‐3T3 cell proliferation

Zhou

Chen

et al. 2018

Biopolymers

View full text Add to dashboard Cite

The recombinant basic fibroblast growth factor (bFGF) containing collagen-binding domain (CBD) has been found to be a potential therapeutic factor in tissue regeneration. However, its binding efficiency and quantification remain uncertain. In this research, massive recombinant bFGFs with good bioactivity for enhancing the proliferation of NIH-3T3 cells were achieved. An ELISA-based quantitative method was set up to investigate the binding efficiency of CBD-bFGFs on collagen films. It indicated that the CBDs significantly increased the collagen-binding ability of bFGF (P < .05), with the optimum binding condition first determined to be in the pH range of 7.5-9.5 (P < .05). Then, the relevant equations to calculate the binding density of bFGF, C-bFGF, and V-bFGF were acquired. Analysis confirmed that the bioactivity of immobilized bFGFs was well correlated with the density of growth factor on collagen films. Based on this research, the density of growth factor is a logical and applicable dosage unit for quantification of binding efficiency of growth factors, rather than traditional concentration of soluble growth factors in tissue engineering applications.

show abstract

Section: Resultsmentioning

confidence: 99%

Binding efficiency of recombinant collagen‐binding basic fibroblast growth factors (CBD‐bFGFs) and their promotion for NIH‐3T3 cell proliferation

Zhou

Chen

et al. 2018

Biopolymers

View full text Add to dashboard Cite

show abstract

“…Histocompatibility, one of the most important properties of biomaterials, can be tested by subcutaneous implantation into the peritoneum of rats. PLLA and PA have received much attention for biomaterial production and have been approved by the FDA for use in vivo. These polymers have a low immune response after being implanted into the body.…”

Section: Discussionmentioning

confidence: 99%

Electrospun Heparin‐Loaded Core–Shell Nanofiber Sutures for Achilles Tendon Regeneration In Vivo

Zhou

Jing

et al. 2018

Macromolecular Bioscience

View full text Add to dashboard Cite

Achilles tendon reconstruction surgery is the primary clinical method for repairing acute Achilles tendon ruptures. However, the efficacy of the postoperative healing process and the recovery of physiological function are inadequate. This study examines the healing mechanism of ruptured rat Achilles tendons seamed with heparin-loaded core-shell fiber sutures fabricated via near-field electrospinning. High-heparin-concentration sutures (PPH3.0) perform better than the low-heparin-concentration sutures and commercial sutures (CSs). The PPH3.0 suture recruits fewer inflammatory cells and shows good histocompatibility in peritoneal implantation experiments. Staining of the Achilles tendon rupture repair zone demonstrates that a high heparin concentration in sutures reduces immune-inflammatory responses. Immunohistochemical analysis reveals that the transforming growth factor-β staining scores of the PPH3.0 sutures are not significantly different from those of the corresponding control group but are significantly different from those of the CSs and non-heparin-loaded-suture groups. According to vascular endothelial growth factor (VEGF) analysis, the concentration of VEGF in the group treated with the PPH3.0 suture increases by 37.5% compared with that in its control group. No significant difference in tension strength is observed between the PPH3.0 group and healthy Achilles tendons. These findings illustrate that this novel method effectively treats Achilles tendon rupture and promotes healing and regeneration.

show abstract

“…Despite advances in the understanding of the etiology and pathophysiology of NEC, neonatologists remain frustrated by the dearth of tools for early definitive diagnosis, the paucity of preventive strategies, and the absence of specific treatment modalities. Gut organoid systems, tissue‐engineered intestinal epithelial platforms, and newer tools to study the ENS offer exciting avenues to gain novel mechanistic insights into epithelial barrier function, ENS, and immune function and the impact of the microbiome . Various novel biomarkers have shown promise in prediction and diagnosis.…”

Section: Ongoing Challenges and Summarymentioning

confidence: 99%

Necrotizing enterocolitis

Sanchez

Kadrofske

2019

Neurogastroenterology Motil

View full text Add to dashboard Cite

Necrotizing enterocolitis (NEC) is an acute inflammatory disease of the intestine which primarily affects preterm infants and is a leading cause of morbidity and mortality in the neonatal intensive care unit. From a clinical standpoint, and during the early course of the disease, NEC can be difficult to distinguish from other diseases and conditions common to the preterm infant, and this warrants the need for specific disease biomarkers. The pathogenesis of NEC is only partly understood but likely involves an altered intestinal barrier immune response to feeding and the developing microbiome. Recent evidence points toward a role of the enteric nervous system in NEC pathogenesis. In this issue, Meister and colleagues use a rodent model of NEC to demonstrate that NEC is associated with diminished vagal tone, as determined by decreased high‐frequency heart rate variability (HF‐HRV), and altered myenteric nitrergic inhibitory neurotransmission. These results augment their previous findings that describe decreased HF‐HRV in human preterm infants with NEC. This mini‐review provides a brief summary of clinical and pathophysiologic aspects of NEC with focus on certain aspects of neurogastroenterology.

show abstract

HB-EGF embedded in PGA/PLLA scaffolds via subcritical CO 2 augments the production of tissue engineered intestine

Cited by 27 publications

References 66 publications

Binding efficiency of recombinant collagen‐binding basic fibroblast growth factors (CBD‐bFGFs) and their promotion for NIH‐3T3 cell proliferation

Binding efficiency of recombinant collagen‐binding basic fibroblast growth factors (CBD‐bFGFs) and their promotion for NIH‐3T3 cell proliferation

Electrospun Heparin‐Loaded Core–Shell Nanofiber Sutures for Achilles Tendon Regeneration In Vivo

Necrotizing enterocolitis

Contact Info

Product

Resources

About