Hazardous Effects of SiO2 Nanoparticles on Liver and Kidney Functions, Histopathology Characteristics, and Transcriptomic Responses in Nile Tilapia (Oreochromis niloticus) Juveniles

Abdel‐Latif, Hany M.R.; Shukry, Mustafa; Euony, Omnia I. El; Soliman, Mohamed Mohamed; Noreldin, Ahmed E.; Ghetas, Hanan A.; Dawood, Mahmoud A.O.; Khallaf, Mohamed A.

doi:10.3390/biology10030183

Cited by 22 publications

(10 citation statements)

References 66 publications

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“…Some in vivo and in vitro studies have shown that SiO 2 NPs can cause toxicity to different organs in the human body; toxicity has been demonstrated in lung epithelial cells [ 9 ], liver cells [ 10 ], intestinal cells [ 11 ], the lungs [ 12 ], and kidneys [ 13 ]. In addition, SiO 2 NPs were found to induce genotoxicity and alterations in gene and protein expression [ 12 , 14 ], and Chen et al [ 15 ] reported that SiO 2 NPs can induce cornea toxicity.…”

Section: Introductionmentioning

confidence: 99%

Mechanistic study of silica nanoparticles on the size-dependent retinal toxicity in vitro and in vivo

Zhang

Zhao

et al. 2022

J Nanobiotechnol

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Background Silica nanoparticles (SiO2 NPs) are extensively applied in the biomedical field. The increasing medical application of SiO2 NPs has raised concerns about their safety. However, studies on SiO2 NP-induced retinal toxicity are lacking. Methods We investigated the retinal toxicity of SiO2 NPs with different sizes (15 and 50 nm) in vitro and in vivo along with the underlying mechanisms. The cytotoxicity of SiO2 NPs with different sizes was assessed in R28 human retinal precursor cells by determining the ATP content and LDH release. The cell morphologies and nanoparticle distributions in the cells were analyzed by phase-contrast microscopy and transmission electron microscopy, respectively. The mitochondrial membrane potential was examined by confocal laser scanning microscopy. The retinal toxicity induced by SiO2 NPs in vivo was examined by immunohistochemical analysis. To further investigate the mechanism of retinal toxicity induced by SiO2 NPs, reactive oxygen species (ROS) generation, glial cell activation and inflammation were monitored. Results The 15-nm SiO2 NPs were found to have higher cytotoxicity than the larger NPs. Notably, the 15-nm SiO2 NPs induced retinal toxicity in vivo, as demonstrated by increased cell death in the retina, TUNEL-stained retinal cells, retinal ganglion cell degeneration, glial cell activation, and inflammation. In addition, The SiO2 NPs caused oxidative stress, as demonstrated by the increase in the ROS indicator H2DCF-DA. Furthermore, the pretreatment of R28 cells with N-acetylcysteine, an ROS scavenger, attenuated the ROS production and cytotoxicity induced by SiO2 NPs. Conclusions These results provide evidence that SiO2 NPs induce size-dependent retinal toxicity and suggest that glial cell activation and ROS generation contribute to this toxicity. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

Mechanistic study of silica nanoparticles on the size-dependent retinal toxicity in vitro and in vivo

Zhang

Zhao

et al. 2022

J Nanobiotechnol

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“…In our study, there was no significant difference in INF-γ concentration among the groups, and HSP-70 was decreased in the HT and HL groups compared with the control group. In a similar sense, the mRNA expression levels of HSP-70 were increased of the Nile tilapia when exposed to toxicants and stressors ( Abdel-Latif et al, 2021a , b ). Additionally, previous research revealed that probiotics Clostridium butyricum could similarly increase the expression of the IL-10 and HSP-70 in weaning rex rabbits ( Liu et al, 2019 ) and colon cancer HT-29 cells ( Andriamihaja et al, 2009 ; Meng et al, 2021 ).…”

Section: Discussionmentioning

confidence: 86%

The Effect of Dietary Lactic Acid Bacteria on Intestinal Microbiota and Immune Responses of Crucian Carp (Carassius auratus) Under Water Temperature Decrease

Liu

Xu³

et al. 2022

Front. Microbiol.

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Temperature changes have a great impact on fish feeding, intestinal microorganisms, metabolism, and immune function. Therefore, it is necessary to develop effective methods to enhance the survival rates and growth of fish under water temperature changes. Lactic acid bacteria (LAB) are promising immunostimulatory feed additive, as demonstrated by their beneficial effects in several fish species. This study investigated the short-term effects of dietary LAB on intestinal microbiota composition and immune responses of crucian carp (Carassius auratus) when water temperature decreased from 30 ± 1°C to 18 ± 1°C. Lactococcus (L.) lactis 1,209 and L. lactis 1,242 with potential probiotics isolated from the intestine of Qinghai naked carp (Gymnocypris przewalskii) were selected as feed additives for the crucian carp feeding experiment. A total of 225 commercially available healthy crucian carp (250 ± 10 g) of similar age were kept in 30°C water for a week and then immediately transferred to 18 ± 1°C water, assigned to three dietary treatments for a 16-day feeding trial randomly: (1) HC, diets without additives (the control group); (2) HT, diets with 106 CFU/ml L. lactis 1,209; and (3) HL, with 106 CFU/ml L. lactis 1,242. Each group was set up with 3 replicates and each with 25 fish. The results showed that the mortality rate of crucian carp in HC, HT, and HL group was 50, 27, and 33%, respectively. High-throughput sequencing results displayed that the composition of the intestinal microorganism varied dynamically in response to different treatments and water temperature decrease. Among them, compared with the HC group, a higher abundance of Firmicutes and Proteobacteria, and a lower of Actinobacteria appeared in HT and HL. The cytokines heat shock protein 70 (HSP-70) in crucian carp intestinal tract significantly decreased when water temperature decreased (p < 0.05).

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“…The literature (Lu et al, 2010;Yu et al, 2012;Fu et al, 2013;Isoda et al, 2013;Hassankhani et al, 2015;Chatterjee et al, 2016;Abdelhalim et al, 2018;Li et al, 2018;Abdel-Latif et al, 2021;Sun et al, 2021) and our previous studies (Lu et al, 2010;Yu et al, 2012;Fu et al, 2013;Isoda et al, 2013;Hassankhani et al, 2015;Chatterjee et al, 2016;Abdelhalim et al, 2018;Li et al, 2018;Abdel-Latif et al, 2021;Sun et al, 2021) have shown that silica particles with particle sizes of 10-1000 nm cause significant damage to the liver, whereas particles with sizes greater than 100 nm cause significant damage to the kidney. To study the effect of particles on the liver, we selected MSNs with an average particle size of 80 nm with a normal particle size distribution.…”

Section: Discussionmentioning

confidence: 84%

Integrative Metabolomics, Proteomics and Transcriptomics Analysis Reveals Liver Toxicity of Mesoporous Silica Nanoparticles

Sun

et al. 2022

Front. Pharmacol.

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As pharmaceutical excipients, mesoporous silica nanoparticles (MSNs) have attracted considerable concern based on potential risks to the public. The impact of MSNs on biochemical metabolism is poorly understood, and few studies have compared the effects of MSNs administered via different routes. To evaluate the hepatotoxicity of MSNs, metabolomics, proteomics and transcriptomic analyses were performed in mice after intravenous (20 mg/kg/d) or oral ad-ministration (200 mg/kg/d) of MSNs for 10 days. Intravenous injection induced significant hepatic injury based on pathological inspection and increased the levels of AST/ALT and the inflammatory factors IL-6, IL-1β and TNF-a. Omics data suggested intravenous administration of MSNs perturbed the following metabolites: succinate, hypoxanthine, GSSG, NADP+, NADPH and 6-phosphogluconic acid. In addition, increases in GPX, SOD3, G6PD, HK, and PFK at proteomic and transcriptomic levels suggested elevation of glycolysis and pentose phosphate pathway, synthesis of glutathione and nucleotides, and antioxidative pathway activity, whereas oxidative phosphorylation, TCA and mitochondrial energy metabolism were reduced. On the other hand, oral administration of MSNs disturbed inflammatory factors and metabolites of ribose-5-phosphate, 6-phosphogluconate, GSSG, and NADP+ associated with the pentose phosphate pathway, glutathione synthesis and oxidative stress albeit to a lesser extent than intravenous injection despite the administration of a ten-fold greater dose. Overall, systematic biological data suggested that intravenous injection of nanoparticles of pharmaceutical excipients substantially affected hepatic metabolism function and induced oxidative stress and inflammation, whereas oral administration exhibited milder effects compared with intravenous injection.

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Hazardous Effects of SiO2 Nanoparticles on Liver and Kidney Functions, Histopathology Characteristics, and Transcriptomic Responses in Nile Tilapia (Oreochromis niloticus) Juveniles

Cited by 22 publications

References 66 publications

Mechanistic study of silica nanoparticles on the size-dependent retinal toxicity in vitro and in vivo

Mechanistic study of silica nanoparticles on the size-dependent retinal toxicity in vitro and in vivo

The Effect of Dietary Lactic Acid Bacteria on Intestinal Microbiota and Immune Responses of Crucian Carp (Carassius auratus) Under Water Temperature Decrease

Integrative Metabolomics, Proteomics and Transcriptomics Analysis Reveals Liver Toxicity of Mesoporous Silica Nanoparticles

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