Hazard Assessments of Cattle Fascioliasis With Special Reference to Hemato-Biochemical Biomarkers

Hashem, Mohamed A.; Mohamed, Samy S.

doi:10.17140/vmoj-2-111

Cited by 7 publications

(5 citation statements)

References 11 publications

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“…In contrast, the serum total albumin showed significant decrease in infected buffaloes during the period post infection, as shown in the Table 1. The biochemical results of the current study affirmed many previous studies by Hashem and Mohamed (2017), Nasreldin and Zaki (2020), Brahmbhatt et al (2021), concerning effects of Fasciolosis on serum total Bilirubin, Albumin, Creatinine level and active serum enzyme ALT, AST, GGT and ALP during the period post infection. But our results study were conflict with the findings obtains by Rajamanickam et al (1987), Matanović et al (2007), who found a significant decrease in biochemical parameters in infected animals such as creatinine and active serum enzymes AST and ALP during the post-infection period, as well as disagreements with the findings of Ganguly et al (2016), that found a significant increase in the albumin level of infected animals.…”

Section: Resultssupporting

confidence: 90%

“…This variation might be attributed to the migration of juvenile flukes through the liver parenchyma, which causes disruption of hepatocellular integrity, fibrosis, and necrosis, thus, enzymes from damaged cells are released into the blood and their concentration increases in serum AST and ALT in the infected animals (Brahmbhatt et al, 2021). Also, the cellular changes from parasitism increased the permeability of the hepatic cells, which in turn resulted in the release of the enzymes into the serum (Hashem and Mohamed, 2017). In addition, the serum GGT levels found in our study is an indicator of chronic changes, cholestasis, and epithelial damage in bile ducts that may be due to the presence of adult flukes in biliary tract (Hodzic et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

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Biochemical and Histopathological Changes Associated with Fasciola spp. in Slaughtered Buffaloes at Al-Muthanna Province, Iraq

Jasim¹,

Al-Amery²

2023

AAVS

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The aim of this study was to determine the biochemical changes in the infected buffaloes with fasciolosis. Also, the current study recognized the gross and histopathological changes resulting from the invasive Fasciola spp. in the liver and gallbladder. The biochemical results showed statistically significant increases in the serum total bilirubin, creatinine, and active serum enzymes ALT, AST, ALP, and GGT. In contrast, the serum total albumin showed a significant decrease in infected buffaloes. Moreover, the results of the gross pathological examination revealed that cirrhosis and paleness of the liver with multiple abscesses appearing as pale necrotic areas, as well as thickness and calcification with fibrinous exudates of bile ducts, were the most frequent gross lesions. Meanwhile, the histopathological examination showed hyperplasia, fibrous proliferation in the portal area and coagulative necrosis of hepatocytes with large numbers of inflammatory cell infiltration such as eosinophil, Kupffer cell and scattered lymphocytes in the area previously migrated by young flukes as well hepatocytes displayed a fatty change with dilated hepatic sinusoids. Furthermore, our findings show that the bile ducts have hyperplasia, which is associated with fibrous cholangitis and an abundance of inflammatory cells with accumulated bile pigment (hemosiderin) in the dilated bile ducts.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Biochemical and Histopathological Changes Associated with Fasciola spp. in Slaughtered Buffaloes at Al-Muthanna Province, Iraq

Jasim¹,

Al-Amery²

2023

AAVS

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“…Increases in hepatobiliary GGT and hepatocellular GLDH are highly tissue-specific and cannot be attributed to any other organ or system, and are long-known [ 19 , 20 ] to be the most sensitive and specific biochemistry biomarkers for fasciolosis across species. A combination of the above biomarkers may be nearly pathognomonic for fasciolosis [ 21 , 22 ]. Collection of blood samples for haematological and biochemical analysis can easily be carried out in the field, and results returned quickly and with relatively little expense, even when an external laboratory is used.…”

Section: Discussionmentioning

confidence: 99%

Acute fasciolosis in an alpaca: a case report

Hayes¹,

O'Brien

Wolfe

et al. 2021

BMC Vet Res

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Background The popularity of new world camelids, particularly alpacas, is growing rapidly in Ireland, presenting a clinical challenge to veterinary practitioners who may not have worked with these species previously. To the authors’ knowledge, the clinical course of a case of acute fasciolosis in an alpaca has not previously been reported, and fasciolosis has not been reported at all in alpacas in Ireland, making this case report a valuable addition to the current literature. Case presentation A three-year-old male castrated huacaya alpaca was admitted to UCD Veterinary Hospital with a two-day history of colic and tenesmus. He had been treated with albendazole, dexamethasone and potentiated amoxycillin by the referring veterinary practitioner with no response. On initial clinical exam, sensitivity to abdominal palpation was the only abnormality. However, the alpaca proceeded to show abnormal lying positions, tenesmus and reduced faecal output over the next 24 h. A general blood panel demonstrated moderate anaemia, marked hyperglobulinaemia and moderately increased hepatocellular and hepatobiliary enzyme activity. Abdominal radiography revealed enlargement of the first forestomach compartment without evidence of gastrointestinal obstruction or peritonitis. An abdominal ultrasound exam revealed an elongated, heterogenous mass in the caudoventral abdomen that appeared to be contiguous with the liver. FNA of this mass revealed that it was in fact a liver lobe with biliary stasis and inflammation. Faecal sedimentation demonstrated Fasciola hepatica eggs. In spite of treatment with triclabendazole and supportive treatment including blood transfusion, the alpaca’s condition continued to deteriorate and he was euthanised. On post-mortem exam, acute fasciolosis was diagnosed. Conclusions The clinical presentation and course of a case of acute fasciolosis in an individual alpaca is described, including the results of a range of diagnostic tests that were carried out. The final diagnosis is supported by a description of post-mortem findings. This information will serve as a resource for veterinary practitioners involved in the diagnosis and treatment of similar cases.

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“…Proteolytic activities in protein extracts from F. hepatica eggs were investigated also using a biochemical approach. Enzymatic activities were measured using a continuous kinetic assay with a complex library of internally quenched FRET substrates in buffers of a wide pH range (2)(3)(4)(5)(6)(7)(8)(9)(10). Proteolytic activities of extracts derived from eggs of different ages have different pH profiles (Figure 7).…”

Section: Biochemical Characterisation Of Proteolytic Activities Ph Optima and Inhibition Assaysmentioning

confidence: 99%

“…US$ 3.2 billion a year 3 ). The clinical picture of fascioliasis, in both humans and livestock, includes anaemia, bile duct fibrosis and inflammation, liver atrophy, cirrhosis, decrease in bile production, and weight loss [4][5][6][7] .…”

Section: Introductionmentioning

confidence: 99%

Transcriptome and proteome dynamics during the embryonation of Fasciola hepatica eggs

Ilgová

Vorel

Roudnický

et al. 2021

Preprint

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Fasciola hepatica is a global parasite of livestock which also causes a neglected zoonosis in humans. The parasite’s communication with the host during its complicated lifecycle is based on an ingenious enzymatic apparatus which includes a variety of peptidases. These enzymes are implicated in parasite migration, pathogenesis of the disease, and modification of host immune response. Although the dynamics of proteolytic machinery produced by intra-mammalian F. hepatica life stages has been previously investigated in great detail, peptidases of the eggs so far received little scientific attention. In this study, we performed a comparative RNA-seq analysis aimed at identification of peptidases expressed in F. hepatica eggs at different ages and employed mass spectrometry in order to identify and quantify peptidases translated in F. hepatica egg lysates. Finally, we measured and classified proteolytic activities in extracts from F. hepatica eggs using a library of fluorogenic substrates and peptidase class-selective inhibitors. Using these methods, we observed significant changes in the composition of peptidases during egg maturation.

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Hazard Assessments of Cattle Fascioliasis With Special Reference to Hemato-Biochemical Biomarkers

Cited by 7 publications

References 11 publications

Biochemical and Histopathological Changes Associated with Fasciola spp. in Slaughtered Buffaloes at Al-Muthanna Province, Iraq

Biochemical and Histopathological Changes Associated with Fasciola spp. in Slaughtered Buffaloes at Al-Muthanna Province, Iraq

Acute fasciolosis in an alpaca: a case report

Transcriptome and proteome dynamics during the embryonation of Fasciola hepatica eggs

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