Hawthorn extract inhibited the PI3k /Akt pathway to prolong the lifespan of Drosophila melanogaster

Abstract

HE is a natural extract with strong antioxidant capacity. Drosophila melanogaster was used to explore HE could delay aging in this study. We detected that 3 mg/ml HE could increase stress tolerance (heat, cold, starvation, oxidative stress), reduce intestinal dysfunction, and prolong the lifespan of D. melanogaster. Network pharmacology analysis showed HE could act through the PI3K‐Akt pathway. Meanwhile, HE intervention inhibited the gene expression of InR, PI3K, and Akt‐1, and further increased the gene expression of Atg1, Atg5, Atg8a, and Atg8b. Furthermore, HE inhibited the unnatural propagation of ISCs and increased the number of lysosomes. Supplement with HE may be an effective intervention for aging D. melanogaster. Practical applications In recent years, diseases that come with aging have seriously affected people’s healthy life. Hawthorn is a kind of nutrient‐rich substance that is rich in flavonoids and thus has many potential biological and pharmacological functions. Our results showed that HE has good antioxidant properties and can maintain intestinal homeostasis, which provides a good theoretical basis for the development and research using HE as an effective natural antioxidant for the elderly.

Hawthorn fruit extract ameliorates H2O2-induced oxidative damage in neuronal PC12 cells and prolongs the lifespan of Caenorhabditis elegans via the IIS signaling pathway

“…41 A previous study has demonstrated that the longevity of hawthorn extract in Drosophila melanogaster was regulated by the PI3K/AKT pathway. 37 In addition, chlorogenic acid 42 and epigallocatechin 43 mediated the longevity of C. elegans relies on the IIS pathway. In this study, HFE up-regulated the gene level of daf-16 and down-regulated the levels of daf-2 , akt-1 , and age-1 (Fig.…”

“…Similar results were observed in Drosophila melanogaster treated with hawthorn extract. 37 The PI3K/AKT signaling pathway is a part of the insulin/ insulin-like growth factor-1 signaling (IIS) pathway, which is a crucial mechanism for regulating senescence. 38 DAF-2 is located upstream of AGE-1, an ortholog of the mammal insulin/IGF receptor, its down-regulation could increase the…”

Artemisia argyi extract exerts antioxidant properties and extends the lifespan of Drosophila melanogaster

“…Some attention has been attracted to the effects of dietary manipulations on the lifespan of D. melanogaster and other animals. [20][21][22][23][24] These previously published studies suggest that dietary induction may be beneficial in extending the lifespan of fruit flies or other animals.…”

“…reported that activation of individual AMPs, including statin in D. melanogaster , significantly extended the lifespan of the fruit flies. Some attention has been attracted to the effects of dietary manipulations on the lifespan of D. melanogaster and other animals 20‐24 . These previously published studies suggest that dietary induction may be beneficial in extending the lifespan of fruit flies or other animals.…”