Hatchability and mortality following infection of chick embryos with subgroup a rous sarcoma virus

Pani, P.; Kishore, Subodh; Naithani, Sangeeta

doi:10.1080/03079458808436475

Cited by 10 publications

(27 citation statements)

References 15 publications

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“…Most of the LT(+) deaths in WL chicks occurred within 3 weeks from the date of hatching, regardless of pock count ranges (Text-fig, la), as reported earlier in heavy breeds of fowl (Pani et al, 1988). LT mortality trait and CAM phenotype.…”

Section: Methodsmentioning

confidence: 54%

“…CAM-inoculated embryos following the LT-assay (Pani et al, 1988), were hatched and wing-banded. They were brooded in a clean thermo-regulated battery brooder with feed and water ad libitum until the termination of the experiment at 4 weeks of age.…”

Section: Chickensmentioning

confidence: 99%

“…Embryos of replicates 5 to 8 were divided equally into two groups: one group was inoculated with a 0.2 ml dose of virus containing 200 pfu and the other with a 0.2 ml dose containing 20 pfu. Pock counts on CAMs, chick monitoring and autopsy procedures were as described in the previous study (Pani et al, 1988). The autopsy findings of LT(+) and LT(-), i.e.…”

Section: Chickensmentioning

confidence: 99%

“…Eleven-day-old pedigreed WL embryos were inoculated via the CAM (Dougherty et al, 1960;Pani et al, 1988) with 200 pfu of BS-RSV in 0.2 ml of primary cell culture medium. BS-RSV was obtained from Dr L.N.…”

Section: Virus Assaymentioning

confidence: 99%

“…The CAM-assay is recognised as an efficient and reliable method for screening chicken flocks for susceptibility to Rous sarcoma virus (RSV) of subgroup A (Prince, 1958a,b;Crittenden and Motta, 1969;Pani and Biggs, 1973). In a study using four heavy breeds of fowl Pani et al (1988) found that CAMinoculated embryos susceptible to the Bryan standard strain of Rous sarcoma virus (BS-RSV) (subgroup A) died within 3 weeks after hatching from a fibrosarcomatous liver tumour (LT) and that CAM-resistant chicks usually survived. However, unexpectedly, about 21% of the CAM-resistant chicks died of liver tumours whereas some of the C AM-susceptibles survived.…”

Section: Introductionmentioning

confidence: 99%

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Inheritance of fibrosarcomatous liver tumours in White Leghorn chicks inoculated vu the chorioallantoic membrane with subgroup A Rous sarcoma virus: A four‐allele genetic model

Pani

Naithani

1990

Avian Pathology

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SUMMARYAn investigation was made using White Leghorn fowl to study the genetic control of subgroup A Rous sarcoma virus-induced fibrosarcomatous liver tumours (LT) in chicks inoculated via the chorioallantoic membrane (CAM). A total of 723 CAM-inoculated embryos were hatched in three experiments. In Expt 1, CAM-susceptibility and LT-mortality were examined on a within sire family basis to ascertain the inter-relationship between the two paramaters. In Expt 2, sires that had records of high or low LT(+) deaths in the families were selected to produce progeny within high and low incidence lines in the next generation to ascertain the amenability of the trait (LT death) to selection pressure. Survivors of LT-assay (Expt 1) were mated inter se in Expt 3 to study the inheritance of the two traits according to known or proposed genetic models. It was shown that LT mortality is a genetic trait because of its amenability to selection, with a high realised heritability (h 2 R = 1.16). In the three experiments, most CAM-susceptibles (S) died of LT(+), and most CAM-resistants(R) survived, but there were some conversely associated phenotypes i.e. S(LT-) and R(LT+). The conventional 2-allele model of the tva (tumour virus a) locus with pleiotropic effect, or a 2-locus model with linkage, were considered inadequate to explain the occurrence of conversely associated phenotypes on a within family basis. However, a 4-allele model of the tva locus showed a good fit to the results of this study.

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Section: Methodsmentioning

confidence: 54%

Section: Chickensmentioning

confidence: 99%

Section: Chickensmentioning

confidence: 99%

Section: Virus Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inheritance of fibrosarcomatous liver tumours in White Leghorn chicks inoculated vu the chorioallantoic membrane with subgroup A Rous sarcoma virus: A four‐allele genetic model

Pani

Naithani

1990

Avian Pathology

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Genetic control of cellular infection by subgroups A and C RNA tumour viruses in guinea fowl

1993

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An investigation was carried out in guinea fowl to determine their susceptibility to infection by Rous sarcoma viruses of subgroups A and C. A standard dose of each subgroup virus was inoculated into 14-day-old embryos via the chorioallantoic membrane (CAM). On the 10th day after inoculation, 50% of the embryonic chorioallantoic membranes were harvested to assess their infection status (CAM(+) or (-)), while the rest were allowed to hatch. The hatchabilities of the embryos inoculated with subgroups A and C were about 50% and 57%, respectively. The relative sensitivities of guinea fowl to infection by viruses of subgroups A and C were observed to be 0.220 and 0.003, respectively, as compared to chickens (1.00). Mortality due to subgroup A virus-induced liver tumours (LT) was 54% and four phenotypic subclasses, namely CAM(+) LT(+), CAM(+) LT(-), CAM(-) LT(+) and CAM(-) LT(-), were observed in guinea fowl as in chickens. However, a higher incidence (31%) of conversely associated phenotypes, i.e. CAM(+) LT(-) and CAM(-) LT(+), were observed in guinea fowl. Mortality caused by subgroup A virus-induced liver tumours was first observed in inoculated guinea fowl keets during the 3rd week after hatching, and 93% of the mortality occurred within 6 weeks. The peak mortality occurred in the 4th week after hatching. The target organs for transformation were considered to be the liver and spleen because of the equal incidence of tumours in these organs. Males and females were equally likely to die from liver tumours.(ABSTRACT TRUNCATED AT 250 WORDS)

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Genetic susceptibility of indigenous chicks to subgroup A Rous sarcoma virus inoculated via the chorioallantoic membrane

Rout

Pani

Naithani

1992

Veterinary Immunology and Immunopathology

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Hatchability and mortality following infection of chick embryos with subgroup a rous sarcoma virus

Cited by 10 publications

References 15 publications

Inheritance of fibrosarcomatous liver tumours in White Leghorn chicks inoculated vu the chorioallantoic membrane with subgroup A Rous sarcoma virus: A four‐allele genetic model

Inheritance of fibrosarcomatous liver tumours in White Leghorn chicks inoculated vu the chorioallantoic membrane with subgroup A Rous sarcoma virus: A four‐allele genetic model

Genetic control of cellular infection by subgroups A and C RNA tumour viruses in guinea fowl

Genetic susceptibility of indigenous chicks to subgroup A Rous sarcoma virus inoculated via the chorioallantoic membrane

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