Hataedock Treatment Has Preventive Therapeutic Effects in Atopic Dermatitis‐Induced NC/Nga Mice under High‐Fat Diet Conditions

Abstract

This study investigated the preventive therapeutic effects of Hataedock (HTD) treatment on inflammatory regulation and skin protection in AD-induced NC/Nga mice under high-fat diet conditions. Before inducing AD, the extract of Coptidis Rhizoma and Glycyrrhiza uralensis was administered orally to the 3-week-old mice. After that, AD-like skin lesions were induced by applying DNFB. All groups except the control group were fed a high-fat diet freely. We identified the effects of HTD on morphological changes, cytokine release and the induction of apoptosis through histochemistry, immunohistochemistry, and TUNEL assay. HTD downregulated the levels of IL-4 and PKC but increased the levels of LXR. HTD also suppressed the mast cell degranulation and release of MMP-9, Substance P. The levels of TNF-α, p-IκB, iNOS, and COX-2 were also decreased. The upregulation of inflammatory cell's apoptosis is confirmed by our results as increase of apoptotic body and cleaved caspase-3 and decrease of Bcl-2. HTD also reduced edema, angiogenesis, and skin lesion inflammation. Our results indicate HTD suppresses various inflammatory response on AD-induced mice with obesity through the regulation of Th2 differentiation and the protection of lipid barrier. Therefore, HTD could be used as an alternative and preventive therapeutic approach in the management of AD.

Effect of Hataedock Treatment on Epidermal Structure Maintenance through Intervention in the Endocannabinoid System

“…In Korean medicine, Hataedock (HTD), herbal extracts that are orally administered to neonates and infants, was used to clear fetal heat to prevent inflammatory diseases. Our previous studies have shown that HTD mitigates AD development due to fetal heat and controls 2skewed conditions [26,27]. Moreover, our studies showed that lipid barrier formation in the epidermis is increased after the application of HTD [28].…”

Inhibitory Effect of Carnosol on Phthalic Anhydride-Induced Atopic Dermatitis via Inhibition of STAT3

“…In AD, NO, iNOS and COX-2 stimulated by TNF-α/IFN-γ and IL-1β contributed to induce AD ( Orita et al ., 2011 ; Ahn et al ., 2016 ). Some studies such as Hataedock, Quercetin, Resveratrol and K112PC-5 show that anti-AD effects via inhibition of NO production, iNOS, COX-2 and cytokines ( Kang et al ., 2008 ; Karuppagounder et al ., 2014 , 2015; Cha et al ., 2016 ). Our study indicated that carnosol has anti-AD effect through reducing inflammatory responses.…”

Impact of Nutritional Supplementation on Canine Dermatological Disorders

“…Numerous reports in literature investigated the anti-inflammatory effects of Actinidia arguta (hardy kiwi) [50,51]. Chinese herbs, such as Rhemannia glutinosa, Paeonia lactiflora, and Glycyrrhiza uralensis have a significant steroid sparing effect when administered in combination with methylprednisolone for the management of canine AD, in addition to having inflammatory, antioxidant, and antimicrobial properties [52][53][54][55][56][57][58][59][60][61]. Arctium lappa, Althaea officinalis, Malva sylvestris, and Ribes nigrum also are attributed with antioxidant and anti-inflammatory activities [62].…”