Harvesting, processing, and evaluation of in vitro-manipulated equine preantral follicles: A review

Gastal, E.L.; Aguiar, F.L.N.; Gastal, G. D. A.; Alves, Kele Amaral; Alves, Bênner Geraldo; Figueiredo, J.R.

doi:10.1016/j.theriogenology.2020.06.044

Cited by 8 publications

(4 citation statements)

References 129 publications

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“…No differences were detected regarding follicle diameter and CL area in agreement with previous studies [ 21 , 22 ]. Consistent with those authors, age is a critical factor that influences the quantity and quality of the equine preantral follicle.…”

Section: Discussionsupporting

confidence: 92%

Assessment of Age Effects on Ovarian Hemodynamics Using Doppler Ultrasound and Progesterone Concentrations in Cycling Spanish Purebred Mares

Requena

Campos

Marín

et al. 2021

Animals

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In equine reproduction, accurate and timely detection of the moment of ovulation is of great importance. Power Doppler ultrasound technology is a non-invasive method that enables to assess the morpho-echogenic features and blood flow changes during the estral cycle in mares. The objective of the present study was to evaluate the influence of age on ultrasonographic parameters (follicular diameter, follicular blood flow—FBF, corpus luteum (CL) area and corpus luteum blood flow—CLBF) and blood plasma progesterone concentrations in cycling Spanish Purebred mares (15 less than 8 years old and 15 equal o higher than 8 years old). The ultrasound images obtained were analyzed with the Image Colour Summarizer software, which allows the quantification of the pixels of each image. Young mares had significantly higher FBF, CLBF and plasma progesterone levels. Moreover, linear regression analysis showed that blood progesterone levels could be predicted in both groups from CLBF with moderate precision and accuracy. In conclusion, Power Doppler was useful to assess ovarian hemodynamics. Our results support that age is a factor that significantly influences FBF and CLBF as well as blood progesterone concentration in mares. More studies would be needed to develop high precision and accuracy predictive models of blood progesterone concentration from CLBF measured by Power Doppler.

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Section: Discussionsupporting

confidence: 92%

Assessment of Age Effects on Ovarian Hemodynamics Using Doppler Ultrasound and Progesterone Concentrations in Cycling Spanish Purebred Mares

Requena

Campos

Marín

et al. 2021

Animals

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“…In this context, the mare is a particularly appealing model to study folliculogenesis and follicle population, due to shared similarities with both women and other livestock species, making the mare a valuable dual-purpose, dual-benefit animal model (for review, see Carnevale 2008 , Mihm & Evans 2008 , Ginther 2012 , Carnevale et al 2020 , Gastal et al . 2020 , Benammar et al 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Preantral follicle population and distribution in the horse ovary

Hyde

Aguiar

Alves

et al. 2022

Reproduction and Fertility

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Characterization of the ovarian preantral follicle population is a necessary step to improve understanding of folliculogenesis and ovarian physiology. Therefore, in the present study, the preantral follicle population in the equine ovary in young and old mares was investigated according to follicular morphology, follicular class, distance from the geometric center using ovarian maps, and follicular density within ovarian portions (lateral vs. intermediary) and regions (dorsal vs. ventral). Ovaries were collected from an abattoir and histologically processed for evaluation, and the follicle population was calculated. Overall, in the current detailed study, a higher preantral follicle population per mare ovary (mean, 82,206 ± 50,022; range, 1,477 to 773,091) than originally reported was identified. Additionally, a mare age effect was observed on the follicle population (young: 152,664 vs. old: 11,750) and the spatial distribution of morphologically normal and abnormal follicles and the density and population of follicular classes. These results demonstrate that, in addition to the preantral follicle population in the mare ovary being comparable to that of other species, the location and spatial distribution of these follicles is dynamic and varies depending on mare age and follicle status (i.e., morphology and developmental stage). The characterization of the distribution and population of preantral follicles in the mare ovary provided by this study can potentially aid in improving reproductive studies and assisted reproductive techniques and may expand the understanding of mechanisms involving ovarian plasticity and follicular migration.

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“…Due to this fact, it is imperative to improve knowledge surrounding follicular activation with the aim to recover the preantral follicle population before depletion, potentially extending female reproductive lifespan via assisted reproductive techniques (ARTs). In this regard, the mare, despite possessing unique ovarian anatomy, has become a powerful animal model due to its capability to provide insight into ovarian function and physiology for women and other livestock species (for review, see [3][4][5][6]).…”

Section: Introductionmentioning

confidence: 99%

Characterization of preantral follicle clustering and neighborhood patterns in the equine ovary

et al. 2022

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Understanding the transition from quiescent primordial follicles to activated primary follicles is vital for characterizing ovarian folliculogenesis and improving assisted reproductive techniques. To date, no study has investigated preantral follicle crowding in the ovaries of livestock or characterized these crowds according to follicular morphology and ovarian location (portions and regions) in any species. Therefore, the present study aimed to assess the crowding (clustering and neighborhood) patterns of preantral follicles in the equine ovary according to mare age, follicular morphology and developmental stage, and spatial location in the ovary. Ovaries from mares (n = 8) were collected at an abattoir and processed histologically for evaluation of follicular clustering using the Morisita Index and follicular neighborhoods in ovarian sections. Young mares were found to have a large number of preantral follicles with neighbors (n = 2,626), while old mares had a small number (n = 305). Moreover, young mares had a higher number of neighbors per follicle (2.6 ± 0.0) than old mares (1.2 ± 0.1). Follicle clustering was shown to be present in all areas of the ovary, with young mares having more clustering overall than old mares and a tendency for higher clustering in the ventral region when ages were combined. Furthermore, follicles with neighbors were more likely to be morphologically normal (76.5 ± 6.5%) than abnormal (23.5 ± 6.5%). Additionally, morphologically normal activated follicles had increased odds of having neighbors than normal resting follicles, and these normal activated follicles had more neighbors (2.6 ± 0.1) than normal resting follicles (2.3 ± 0.1 neighbors). In the present study, it was demonstrated that preantral follicles do crowd in the mare ovary and that clustering/neighborhood patterns are dynamic and differ depending on mare age, follicular morphology, and follicular developmental stage.

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Harvesting, processing, and evaluation of in vitro-manipulated equine preantral follicles: A review

Cited by 8 publications

References 129 publications

Assessment of Age Effects on Ovarian Hemodynamics Using Doppler Ultrasound and Progesterone Concentrations in Cycling Spanish Purebred Mares

Assessment of Age Effects on Ovarian Hemodynamics Using Doppler Ultrasound and Progesterone Concentrations in Cycling Spanish Purebred Mares

Preantral follicle population and distribution in the horse ovary

Characterization of preantral follicle clustering and neighborhood patterns in the equine ovary

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