Harvesting Chlorella variabilis Biomass Using Moringa oleifera Seed-induced Sedimentation

Ogbonna, Christian N.; Edeh, IfeanyiChukukwu

doi:10.9734/jabb/2018/43608

Cited by 9 publications

(3 citation statements)

References 29 publications

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“…In this study, the CP, DW, CPr and SGR were significantly (p <0.05) increased when the MLE cultivation medium was enriched with NaHCO 3 or kanwa compared to those supplemented only with urea (Table 1). These results could be explained by the fact that the supply of MLE cultivation medium NaHCO 3 or kanwa can improve water quality and increase the quality of S. platensis growth and it will also cause good removal of turbidity in the cultivation media so that the light penetration increases will improve photosynthesis and production of S. platensis (Ogbonna and Chukukwu, 2018;Silva et al, 2020). This may be also due to uptake of nutrients (carbohydrates and ash) and minerals (Na, K, Ca, Mg) brought by MLE and supplemented NaHCO 3 or kanwa which increase cell growth and the metabolism of carbon in the photosynthetic activity of S. platensis (Nweze and Nwafor, 2014a,b).…”

Section: Growth Parametersmentioning

confidence: 99%

Physiological and biochemical traits, antioxidant compounds and some physico-chemical factors of Spirulina platensis cultivation as influenced by Moringa oleifera leaves extract culture medium enriched with sodium bicarbonate and kanwa

Oscar¹,

Fils²,

Kennedy³

et al. 2021

Afr. J. Biotechnol.

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The prospects for using Moringa oleifera leaves extract (MLE) supplemented with different concentrations of kanwa or sodium bicarbonate (NaHCO 3 ) as a low-cost alternative growth medium of Spirulina platensis were evaluated in a small-scale outdoor cultivation system. The present study was aimed to evaluate the potential of MLE growth medium enriched with different concentrations (4 or 8 g L -1) of kanwa and NaHCO 3 on growth, chlorophyll content, biochemical characteristics, antioxidant compounds and some physico-chemical factors. Jourdan's standard medium was taken as control. The results showed that the growth parameters such as cell population, biomass dry weight, cell productivity and specific growth rate were positively affected in MLE cultivation medium enriched with kanwa or NaHCO 3 at different concentration levels. The addition of urea, kanwa or NaHCO 3 in MLE cultivation medium at different concentration levels increased significantly (p< 0.05) the protein content, the peroxidase and polyphenol oxidase activity, the conductivity, pH, total dissolved solids and salinity from 20 to 25 days of cultivation whereas a decrease in carbohydrate and phenol content was recorded during all the period of the experimentation. The highest values of growth parameters were notably in MLE medium supplemented with urea and kanwa at 8 g L -1. The MLE medium enriched with urea and kanwa at 8 g L -1 was shown to be an appropriate growth medium that can be used as a lowcost alternative growth medium for commercial cultivation of S. platensis.

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Section: Growth Parametersmentioning

confidence: 99%

Physiological and biochemical traits, antioxidant compounds and some physico-chemical factors of Spirulina platensis cultivation as influenced by Moringa oleifera leaves extract culture medium enriched with sodium bicarbonate and kanwa

Oscar¹,

Fils²,

Kennedy³

et al. 2021

Afr. J. Biotechnol.

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“…However, one of the dif f iculties in microalgae biomass utilization is the problem of harvesting (Ogbonna and Edeh, 2018;Ogbonna and Chioke, 2018;Ogbonna and Nwoba, 2021). The cost of harvesting microalgae biomass f or use as f eed stock f or lipids and other bioactive compound extraction accounts f or about 10 -15% of the total production cost (Ogbonna and Nwoba, 2021;Fasaei et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…The use of selff locculating strains of microalgae (Li et al, 2021) or bio-based f locculants such as microbial f locculants or f locculants of plant origin are safe and environmentally f riendly method s (Ogbonna and Nwoba, 2021). Plant seeds and plant vegetative parts have been employed in harvesting microalgae (Ogbonna and Edeh, 2018;Ogbonna and Chioke, 2018;Mainho et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Moringa oleifera seed extract-mediated flocculation as an efficient method for harvesting Chlorella lewinii biomass

Ikele¹,

Ogbonna²

2022

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The aim of this research was to investigate the potentials of harvesting Chlorella lewinii through flocculation by Moringa oleifera seed extract. Water, ethanol, and sodium chloride solution were used to extract flocculating agents from both whole and de-fatted Moringa oleifera seeds and their ability to flocculate C. lewinii cells were evaluated. The effects of extracting solvents, extract concentration, incubation period and culture age on flocculation efficiency were investigated. The lipid contents of the biomass harvested by flocculation using M. oleifera seed extract were compared with those harvested by centrifugation. The results showed that 1M sodium chloride solution was the most effective solvent for extracting M. oleifera active ingredient. The optimum extract concentration was 600±0.10 mg/L with approximately 60±0.46 % efficiency, while the optimum length of period for incubating a mixture of cell culture and seed extract was 80±0.26 min. Defatted Moringa oleifera seed extract was more efficient than whole seed extract with an efficiency of 50%. The percentage lipid content of biomass harvested by centrifuge and moringa extracts decreased in the following order: Centrifugation-(22.55%) > de-fatted seed extract (16.63%) > whole seed extract (14.42%). These results indicate that M. oleifera seed extract is a reliable method of harvesting microalgae biomass.

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Industrial Wastewater to Biohydrogen Production via Potential Bio-refinery Route

Das

Deepti

Purkait

2023

Biorefinery for Water and Wastewater Treatment

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Harvesting Chlorella variabilis Biomass Using Moringa oleifera Seed-induced Sedimentation

Cited by 9 publications

References 29 publications

Physiological and biochemical traits, antioxidant compounds and some physico-chemical factors of Spirulina platensis cultivation as influenced by Moringa oleifera leaves extract culture medium enriched with sodium bicarbonate and kanwa

Physiological and biochemical traits, antioxidant compounds and some physico-chemical factors of Spirulina platensis cultivation as influenced by Moringa oleifera leaves extract culture medium enriched with sodium bicarbonate and kanwa

Moringa oleifera seed extract-mediated flocculation as an efficient method for harvesting Chlorella lewinii biomass

Industrial Wastewater to Biohydrogen Production via Potential Bio-refinery Route

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