Harvesting carbohydrate-rich Arthrospira platensis by spontaneous settling

Depraetere, Orily; Pierre, Guillaume; Deschoenmaeker, Frédéric; Badri, Hanène; Foubert, Imogen; Leys, Natalie; Μάρκου, Γιώργος; Wattiez, Ruddy; Michaud, Philippe; Muylaert, Koenraad

doi:10.1016/j.biortech.2014.12.084

Cited by 44 publications

(22 citation statements)

References 31 publications

(45 reference statements)

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“…Several methods have been tested to harvest microalgae, including gravity sedimentation (Depraetere et al, 2015), centrifugation (Chen et al, 2015), filtration (Nurra et al, 2014) and chemical flocculation (Reyes and Labra, 2016). Gravity settling is simple but only suitable to harvest microalgae with large size (Park and Craggs, 2010).…”

Section: Introductionmentioning

confidence: 99%

Synergy of flocculation and flotation for microalgae harvesting using aluminium electrolysis

Shi

Zhu

Chen

et al. 2017

Bioresource Technology

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Microalgae are often used as feedstock for renewable biofuel production and as pollutant up-takers for wastewater treatment; however, biomass harvesting still remains a challenge in field applications. In this study, electro-flocculation using aluminium electrolysis was tested as a method to collect Chlorella vulgaris. The electrolysis products were positively charged over a wide pH range below 9.5, which gave them a flocculation potential for negatively charged microalgae. As flocculants were in-situ generated and gradually released, microalgae flocs formed in a snowballing mode, resulting in the compaction of large flocs. When higher current density was applied, microalgae could be harvested more rapidly, although there was a trade-off between a higher energy use and more residual aluminium in the culture medium. Benefits of this flocculation method are twofold: the phosphate decrease in post-harvesting could improve nutrient removal in microalgae based wastewater treatment, while the ammonium increase may favor microalgae recovery for medium recycling.

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Section: Introductionmentioning

confidence: 99%

Synergy of flocculation and flotation for microalgae harvesting using aluminium electrolysis

Shi

Zhu

Chen

et al. 2017

Bioresource Technology

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“…Since the accumulation of lipids in Arthrospira is low, as in other cyanobacteria, it is not attractive for biodiesel production [ 6 ]. Nevertheless, this microorganisms is able to accumulate up to 70% of carbohydrates in its biomass under nitrogen (N) limitation, mostly as glycogen granules [ 7 – 12 ]. The advantages of Arthrospira cultures ( i .…”

Section: Introductionmentioning

confidence: 99%

Trade-Off between Growth and Carbohydrate Accumulation in Nutrient-Limited Arthrospira sp. PCC 8005 Studied by Integrating Transcriptomic and Proteomic Approaches

et al. 2015

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Cyanobacteria have a strong potential for biofuel production due to their ability to accumulate large amounts of carbohydrates. Nitrogen (N) stress can be used to increase the content of carbohydrates in the biomass, but it is expected to reduce biomass productivity. To study this trade-off between carbohydrate accumulation and biomass productivity, we characterized the biomass productivity, biomass composition as well as the transcriptome and proteome of the cyanobacterium Arthrospira sp. PCC 8005 cultured under N-limiting and N-replete conditions. N limitation resulted in a large increase in the carbohydrate content of the biomass (from 14 to 74%) and a decrease in the protein content (from 37 to 10%). Analyses of fatty acids indicated that no lipids were accumulated under N-limited conditions. Nevertheless, it did not affect the biomass productivity of the culture up to five days after N was depleted from the culture medium. Transcriptomic and proteomic analysis indicated that de novo protein synthesis was down-regulated in the N-limited culture. Proteins were degraded and partly converted into carbohydrates through gluconeogenesis. Cellular N derived from protein degradation was recycled through the TCA and GS-GOGAT cycles. In addition, photosynthetic energy production and carbon fixation were both down-regulated, while glycogen synthesis was up-regulated. Our results suggested that N limitation resulted in a redirection of photosynthetic energy from protein synthesis to glycogen synthesis. The fact that glycogen synthesis has a lower energy demand than protein synthesis might explain why Arthrospira is able to achieve a similar biomass productivity under N-limited as under N-replete conditions despite the fact that photosynthetic energy production was impaired by N limitation.

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“…The highest contents in carbohydrates, (39.5 ± 2.34) mg.g -1 and (38.73 ± 2.55) mg.g -1 , were observed under conditions 5 (1,125 gL -1 NaNO 3 and 600 μE.m -2 .s -1 ) and 9 (2 gL -1 NaNO 3 and 1000 μE.m -2 .s -1 ). According to Depraetere et al (2015), when nitrate source is depleted, the protein synthesis is reduced. In the present work, the highest concentrations in proteins, 7.05 ±0.30 mg.g -1 and 6.46 ± 0.20 mg.g -1 , were observed for intermediate values of NaNO 3 , in the conditions 5 (1.125 gL -1 of NaNO 3 and 600 μE.m -2 .s -1 ) and 8 (1.125 gL -1 of NaNO 3 and 1000 μE.m -2 .s -1 ), respectively.…”

Section: Carbohydrate and Protein Quanti Cationmentioning

confidence: 99%

Influence of culture conditions on the production of extracellular polymeric substances (EPS) by Arthrospira platensis

Silva

Azero

Teixeira

et al. 2020

Preprint

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Arthrospira platensis is a cyanobacterium that exhibits a large biotechnological interest for the food industry because of its high protein content, pigments, lipids and carbohydrates. The extracellular polymeric substances (EPS) are co-products of secondary metabolism that may present thickening or gelling property. A 3-level factorial design was used to study the combined effect of different nitrate concentrations and photon flux density (PFD) to evaluate the biomass and EPS production by Arthrospira platensis. The best result for biomass production was obtained under condition 6 (2 g.L-1 NaNO3 and 600 µE.m-2s-1) leading a biomass concentration of 1.292 mg.L-1. Condition 1 (0.25 g.L-1NaNO3 and 200 µE.m-2s-1) produced the major EPS yield (111 mg.g-1) followed by condition 9 (2 g.L-1NaNO3 and 1000 µE.m-2s-1). The present of carboxylate and sulfate functional groups was identified in EPS samples. Rheological studies performed for some EPS products at 5 and 10 g.L-1 concentrations revealed a dilute solution behavior.

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Harvesting carbohydrate-rich Arthrospira platensis by spontaneous settling

Cited by 44 publications

References 31 publications

Synergy of flocculation and flotation for microalgae harvesting using aluminium electrolysis

Synergy of flocculation and flotation for microalgae harvesting using aluminium electrolysis

Trade-Off between Growth and Carbohydrate Accumulation in Nutrient-Limited Arthrospira sp. PCC 8005 Studied by Integrating Transcriptomic and Proteomic Approaches

Influence of culture conditions on the production of extracellular polymeric substances (EPS) by Arthrospira platensis

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