Harpertrioate A, an A,B,D-seco-Limonoid with Promising Biological Activity against Alzheimer’s Disease from Twigs of Harrisonia perforata (Blanco) Merr.

Abstract

Harpertrioate A (1), an A,B,D-seco-limonoid with a rearranged ring B incorporating exocyclic C-30, was isolated from the EtOAc extract of Harrisonia perforata twigs. Its structure, including absolute configurations, was determined on the basis of spectroscopic data and X-ray crystallography. This compound exhibited biological activities against Alzheimer’s disease by reducing Aβ42 and Aβ40 production and shifting APP processing toward nonamyloidogenic pathway. The effect of 1 on the Aβ production was comparable to that of gemfibrozil.

Jatrophane Diterpenoids from Euphorbia peplus Linn. as Activators of Autophagy and Inhibitors of Tau Pathology

“…Western blot assays were performed according to the methods in our previous published studies [ 12 , 16 , 41 ]. In brief, cell lysates of U251-MAPT P301S cells were prepared, and protein concentration was determined with the BCA protein assay kit (Beyotime Institute of Biotechnology, P0012).…”

New Monoterpenoid Indole Alkaloids from Tabernaemontana crassa Inhibit β-Amyloid42 Production and Phospho-Tau (Thr217)

“…Western blotting was performed as in our previous studies [ 14 , 38 , 39 , 56 ]. In brief, cell lysates of U251-APP cells were prepared using protein lysis buffer (Beyotime Institute of Biotechnology, P0013).…”

“…Among these avenues, inhibitors of amyloid precursor protein (APP) protease, β-site cleaving enzyme 1 (BACE1), and cyclin-dependent kinase 5 (CDK5) contributing to tau phosphorylation have been suggested as appealing drug targets for AD [ 9 , 10 , 11 , 12 ]. Previous studies have shown that natural products exhibited biological activities against AD by inhibiting cyclin-dependent kinase 5 and tau phosphorylation and reducing A β 42 and A β 40 production toward the nonamyloidogenic pathway [ 13 , 14 ].…”

(±)-Spiroganoapplanin A, a complex polycyclic meroterpenoid dimer from Ganoderma applanatum displaying potential against Alzheimer's disease

“…Inspired by the findings above and our previous studies, 21 we systematically investigated the GMs from the fruiting bodies of G. applanatum and isolated (±)-spiroganoapplanin A ( 1 ) possessing a complex 6/5/5/6/5/6-fused polycyclic skeleton (Fig. 1).…”