Harnessing the power of cellulolytic nitrogen-fixing bacteria for biovalorization of lignocellulosic biomass

Harindintwali, Jean Damascene; Wang, Fang; Yang, Wenhua; Zhou, Jianli; Muhoza, Bertrand; Mugabowindekwe, Maurice; Yu, Xiaobin

doi:10.1016/j.indcrop.2022.115235

Cited by 11 publications

(5 citation statements)

References 115 publications

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“…are saprophytes and are not involved in a disease process [56,57]. The gut of Eisenia fetida is colonized by various anaerobic N 2 -fixing bacteria belonging to the Clostridiaceae family, such as Clostridium butyricum, Clostridium beijerinckii, and Clostridium paraputrificum, which are well-known as nitrogen fixers and cellulose digesters [58][59][60][61]. Earthworms' gut microbiome consists of "nitrogen-fixing" and "decomposer microbes" that are excreted along with nutrients in their vermicasts [12].…”

Section: Microbial Populationsmentioning

confidence: 99%

Physicochemical Changes and Microbiome Associations during Vermicomposting of Winery Waste

et al. 2023

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Annually, the wine industry produces high amounts of waste that can be toxic if disposed of without pretreatment. Vermicomposting is an efficient and low-cost method of decomposing organic matter using earthworms under controlled conditions. The organic substrate used in the vermicomposting process affects microbial populations and reflects the dynamics of enzymatic activity, decomposition of organic matter, and nitrogen transformations. However, the microbiome associations during the whole process are still unexplored. Thus, the aim of the present study was to investigate physicochemical, enzymatic, microbial, and microbiome activities during vermicomposting of winery waste. In this vein, a rectangular vermireactor with Eisenia andrei and Eisenia fetida earthworms, loaded with winery waste, was used. At the end of the process, the carbon/nitrogen (C/N) ratio was decreased, the total nitrogen was increased, the pH was neutralized and excess enzymatic activities were recorded. The bacterial and fungal phyla detected by next-generation sequencing analysis identified Armatimonadetes, Bacteriodetes, Candidatus saccharibacteria, Chloroflexi, Cyanobacteria, Planctomycetes, and Proteobacteria and Ascomycota, Basidiomycota, Chytridiomycota, Entomophthoromycota, Glomeromycota, and Mucoromycota, respectively. Physicochemical and microbial changes occurring during vermicomposting of winery waste, along with the microbiome diversity at the beginning and end of the process, may lead to a better understanding of winery-waste biotransformation into effective biofertilizer.

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Section: Microbial Populationsmentioning

confidence: 99%

Physicochemical Changes and Microbiome Associations during Vermicomposting of Winery Waste

et al. 2023

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“…On the other hand, in the guts of earthworms, in addition to the presence of aerobic bacteria, the presence of anaerobic bacteria, including species such as Bacillus and Clostridium, has been proven [61]. The presence of anaerobic bacteria from the Clostridiaceae family, such as Clostridium butyricum, Clostridium beijerinckii, and Clostridium paraputrificum, known as nitrogen fixers and cellulose digesters [67,89,90], further confirms the diversity of the beneficial microorganisms in the vermicomposting process. Therefore, it is essential to continue research in this field to better understand the exact dynamics and mechanisms of vermicomposting's impact on microorganisms.…”

Section: Discussionmentioning

confidence: 93%

Comparative Study of Vermicomposting: Apple Pomace Alone and in Combination with Wheat Straw and Manure

Kureljušić,

Vesković Moračanin,

Đukić

et al. 2024

Agronomy

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Considering the sporadic number of scientific studies on vermicomposting apple pomace waste, this research conducts a comparative analysis of vermicomposting processes using Eisenia fetida, focusing on apple pomace both independently and in combination with wheat straw and/or manure (experiment 1: 60% apple pomace and 40% cattle manure; experiment 2: 60% wheat straw and 40% cattle manure; experiment 3: 80% apple pomace, 10% wheat straw, and 10% cattle manure; and experiment 4: 100% apple pomace). After a 240-day substrate transformation period, all four variations of vermicompost produced demonstrated favorable sensory properties, along with high microbiological and physicochemical quality. Throughout the vermicomposting process, the pH of all vermicomposting mixtures changed, converging towards approximately neutral values by the process’s conclusion. There was an increase in dry matter content, as well as total N, P, K, Ca, and Mg, along with organic matter. Notably, the levels of heavy metals (Zn, Cu, Cd, and Pb) in both the vermicomposting materials and resulting vermicomposts remained significantly below the maximum permissible levels stipulated by Republic of Serbia and European Union legislation, which is directly linked to the ecological origin of the raw materials used. The microbiological quality of the final vermicomposts was deemed satisfactory. Over time, there was a decrease in the counts of aerobic mesophilic bacteria as well as Escherichia coli. The counts of sulfite-reducing clostridia in all substrates remained below 102 CFU/g, while Salmonella spp. and Listeria monocytogenes were not detected in either the composting materials or the resulting composts. The vermiculture of apple pulp exhibited advantageous characteristics, notably a shortened vermicomposting period (150 days) compared to other agricultural waste. This reduction in processing time contributes an additional layer of advantage to the overall quality and efficiency of the resulting vermicompost.

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“…It is manifested by the phenomenon that the nine ancestral halls all contain these pathways, and the content is extremely high in Qingxi Ancestral Hall and Mingmin Ancestral Hall when compared with other ones, indicating that the surfaces of all ancestral halls have the basic potential to achieve nitrogen fixation (Table S3). It showed that these microbial groups have a relatively strong ability to fix nitrogen and produce ammonium salt, and the presence of ammonium salt will promote microorganisms’ degradation of wood cellulose, which will make it easier for the microbiome to obtain essential nutrients from wood, and further damage the wooden structure of ancestral halls 25 .…”

Section: Resultsmentioning

confidence: 99%

Insights into the Microbial Weathering of Chinese Wooden Ancestral Halls in Guangdong Province

Liu

Wen

et al. 2023

Preprint

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Wooden buildings are facing biodeterioration because of natural weathering. Ancestral halls are traditional Chinese wooden architecture with high artistic value. The research analyzed nine ancestral halls with different visitor flow rates in Guangdong Province under subtropical monsoon climates. With amplicon sequencing and conventional culturing methods, a common core harmful microorganism group which are proven to be capable of degrading cellulose and lignin was found, and some of them originated from human activities, including Bacillus, Pseudomonas, Paenibacillus, Acinetobacter, Toxicocladosporium, Cladosporium, Aspergillus, and Epicoccum, allowing us to apply unified antimicrobial methods to different ancestral halls in Guangdong Province. Additionally, the microbial community is similar between damaged and undamaged points, predicting the potential risk of taking paint coating as the only method for antimicrobial preservation. Hence, we evaluated the effect of four representative biocides and determined the feasibility of low-concentration Isothiazolinone. This research adds significant reference for the protection of wooden buildings.

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Harnessing the power of cellulolytic nitrogen-fixing bacteria for biovalorization of lignocellulosic biomass

Cited by 11 publications

References 115 publications

Physicochemical Changes and Microbiome Associations during Vermicomposting of Winery Waste

Physicochemical Changes and Microbiome Associations during Vermicomposting of Winery Waste

Comparative Study of Vermicomposting: Apple Pomace Alone and in Combination with Wheat Straw and Manure

Insights into the Microbial Weathering of Chinese Wooden Ancestral Halls in Guangdong Province

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