Harnessing the potential of CRISPR-based platforms to advance the field of hospital medicine

McCarthy, Matthew W

doi:10.1080/14787210.2020.1761333

Cited by 6 publications

(3 citation statements)

References 103 publications

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“…The flexibility of this tool has shown potential in understanding biology, pathogenesis and virulence factors in medically important fungi in research settings. Kwon et al ( 2019 ) evaluated the possibilities of using this method in the application stage for different fungal species, while McCarthy ( 2020 ) tested for medically important fungi. Accurate and fast identification is paramount for preventing and treating fungal diseases.…”

Section: Successful Application Of Crispr-cas9 In Medical Mycologymentioning

confidence: 99%

Ten decadal advances in fungal biology leading towards human well-being

et al. 2022

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Fungi are an understudied resource possessing huge potential for developing products that can greatly improve human well-being. In the current paper, we highlight some important discoveries and developments in applied mycology and interdisciplinary Life Science research. These examples concern recently introduced drugs for the treatment of infections and neurological diseases; application of –OMICS techniques and genetic tools in medical mycology and the regulation of mycotoxin production; as well as some highlights of mushroom cultivaton in Asia. Examples for new diagnostic tools in medical mycology and the exploitation of new candidates for therapeutic drugs, are also given. In addition, two entries illustrating the latest developments in the use of fungi for biodegradation and fungal biomaterial production are provided. Some other areas where there have been and/or will be significant developments are also included. It is our hope that this paper will help realise the importance of fungi as a potential industrial resource and see the next two decades bring forward many new fungal and fungus-derived products.

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Section: Successful Application Of Crispr-cas9 In Medical Mycologymentioning

confidence: 99%

Ten decadal advances in fungal biology leading towards human well-being

et al. 2022

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“…Other methodologies not found in our survey, but with promising features are CRISPR-based methodologies (110)(111)(112)(113), lateral flow immunoassay (5), viscoelastic testing (114), and biosensors for COVID-19 (115), being conceived as alternatives to the usual methods. In the patents identified, isothermal amplification proved to be a faster, simpler, and more sensitive method for detecting SARS-CoV-2 (116)(117)(118)(119).…”

Section: Alternative Test Methodologiesmentioning

confidence: 99%

Trends in MERS-CoV, SARS-CoV, and SARS-CoV-2 (COVID-19) Diagnosis Strategies: A Patent Review

Nascimento

Santos

Oliveira

et al. 2020

Front. Public Health

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The emergence of a new coronavirus (SARS-CoV-2) outbreak represents a challenge for the diagnostic laboratories responsible for developing test kits to identify those infected with SARS-CoV-2. Methods with rapid and accurate detection are essential to control the sources of infection, to prevent the spread of the disease and to assist decision-making by public health managers. Currently, there is a wide variety of tests available with different detection methodologies, levels of specificity and sensitivity, detection time, and with an extensive range of prices. This review therefore aimed to conduct a patent search in relation to tests for the detection of SARS-CoV, MERS-CoV, and SARS-CoV-2. The greatest number of patents identified in the search were registered between 2003 and 2011, being mainly deposited by China, the Republic of Korea, and the United States. Most of the patents used the existing RT-PCR, ELISA, and isothermal amplification methods to develop simple, sensitive, precise, easy to use, low-cost tests that reduced false-negative or false-positive results. The findings of this patent search show that an increasing number of materials and diagnostic tests for the coronavirus are being produced to identify infected individuals and combat the growth of the current pandemic; however, there is still a question in relation to the reliability of the results of these tests.

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“…The prokaryotic defense system named “clustered regularly interspaced short palindromic repeats” (CRISPR) machinery, which utilizes segments of nucleic acid containing short, repetitive base sequences and enzymes (Cas), has been exploited for genome editing, known as CRISPR-Cas9, as well as CRISPR-Cas12a, and CRISPR-13 [ 118 ]. Recent insights into the biology of CRISPR/Cas led to new molecular diagnostics that leverage the activity of Cas effectors for sensitive, specific, and rapid nucleic acid detection [ 119 ].…”

Section: Nucleic Acid-based Testsmentioning

confidence: 99%

The Main Molecular and Serological Methods for Diagnosing COVID-19: An Overview Based on the Literature

Machado

Hodel

Barbosa-Júnior

et al. 2020

Viruses

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Diagnostic tests have been considered as the main alternative for the control of coronavirus disease (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), as a correct diagnosis allows for decision making when facing the disease, particularly as there is a lack of effective therapeutic protocols and vaccines. Thus, in this review, we summarized the main diagnostic approaches currently available for the diagnosis of SARS-CoV-2 infection in humans based on studies available in article databases. The tests can be organized into two main categories: nucleic acid-based tests, recommended for the initial detection of the virus, and serological tests, recommended for assessing the disease progression. The studies have shown that the performance of diagnostic methods depends on different factors, such as the type of samples and the characteristics of each assay. It was identified that the positivity of the tests is mainly related to the onset of symptoms. We also observed that point-of-care diagnoses are considered as one of the main trends in this area, due to the low-cost and simplicity of the assay; however, the analytical performance must be critically analyzed. Thus, the COVID-19 pandemic has highlighted the critical role of diagnostic technologies in the control of infectious diseases.

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Harnessing the potential of CRISPR-based platforms to advance the field of hospital medicine

Cited by 6 publications

References 103 publications

Ten decadal advances in fungal biology leading towards human well-being

Ten decadal advances in fungal biology leading towards human well-being

Trends in MERS-CoV, SARS-CoV, and SARS-CoV-2 (COVID-19) Diagnosis Strategies: A Patent Review

The Main Molecular and Serological Methods for Diagnosing COVID-19: An Overview Based on the Literature

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