Harnessing the potential: advances in cyanobacterial natural product research and biotechnology

Baunach, Martin; Guljamow, Arthur; Miguel-Gordo, María; Dittmann, Elke

doi:10.1039/d3np00045a

Cited by 5 publications

(1 citation statement)

References 161 publications

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“…The identification of only 14% of the potential Nostoc secretome, in comparison to 62% in Synechocystis , is curious due to the fact that Nostoc encodes for a much larger number of secretion systems than Synechocystis 12 . This is in line with previous work showing that symbiotic cyanobacteria, such as Nostoc , exhibit high levels of proteomic plasticity and only express a small fraction of their proteome at any given point in time 30,63,64 . This is also in agreement with the coverage of the respective endoproteomes where 65% and 20% of all proteins in Synechocystis and Nostoc , respectively, have been identified by MS-based proteomics 65,66 .…”

Section: Resultssupporting

confidence: 92%

EXCRETE enables deep proteomics of the microbial extracellular environment

Russo,

Oliinyk,

Pohnert

et al. 2024

Preprint

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Extracellular proteins play a significant role in shaping microbial communities which, in turn, can impact ecosystem function, human health, and biotechnological processes. Yet, for many ubiquitous microbes, there is limited knowledge regarding the identity and function of secreted proteins. Here, we introduce EXCRETE (enhanced exoproteome characterization by mass spectrometry), a workflow that enables comprehensive description of microbial exoproteomes from minimal starting material. Using cyanobacteria as a case study, we benchmark EXCRETE and show a significant increase over current methods in the identification of extracellular proteins. Subsequently, we show that EXCRETE can be miniaturized and adapted to a 96-well high-throughput format. Application of EXCRETE to cyanobacteria from different habitats (Synechocystissp. PCC 6803,Synechococcussp. PCC 11901, andNostoc punctiformePCC 73102), and in different cultivation conditions, identified up to 85% of all predicted secreted proteins. Finally, functional analysis reveals that cell envelope maintenance and nutrient acquisition are central functions of the cyanobacterial secretome. Collectively, these findings challenge the general belief that cyanobacteria lack secretory proteins and point to a functional conservation of the secretome across freshwater, marine, and terrestrial species.

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Section: Resultssupporting

confidence: 92%

EXCRETE enables deep proteomics of the microbial extracellular environment

Russo,

Oliinyk,

Pohnert

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Advancing cyanobacterial biotechnology: new frontiers in natural product discovery and production

Jeyaraj

2024

Natural Product Research

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Green microbes: Potential solutions for key sustainable development goals

Diaz‐Troya,

Huertas

2024

Microbial Biotechnology

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The latest assessment of progress towards the Sustainable Development Goals (SDGs) has identified major obstacles, such as climate change, global instability and pandemics, which threaten efforts to achieve the SDGs even by 2050. Urgent action is needed, particularly to reduce poverty, hunger and climate change. In this context, microalgae are emerging as a promising solution, particularly in the context of food security and environmental sustainability. As versatile organisms, microalgae offer nutritional benefits such as high‐quality proteins and essential fatty acids, and can be cultivated in non‐arable areas, reducing competition for resources and improving the sustainability of food systems. The role of microalgae also includes other applications in aquaculture, where they serve as sustainable alternatives to animal feed, and in agriculture, where they act as biofertilizers and biostimulants. These microorganisms also play a key role in interventions on degraded land, stabilizing soils, improving hydrological function and increasing nutrient and carbon availability. Microalgae therefore support several SDGs by promoting sustainable agricultural practices and contributing to land restoration and carbon sequestration efforts. The integration of microalgae in these areas is essential to mitigate environmental impacts and improve global food security, highlighting the need for increased research and development, as well as public and political support, to exploit their full potential to advance the SDGs.

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Harnessing the potential: advances in cyanobacterial natural product research and biotechnology

Abstract: In order to exploit the enormous natural product potential of cyanobacteria, suitable techniques must be tailored to the special characteristics of the phototrophic prokaryotes.

Cited by 5 publications

References 161 publications

EXCRETE enables deep proteomics of the microbial extracellular environment

EXCRETE enables deep proteomics of the microbial extracellular environment

Advancing cyanobacterial biotechnology: new frontiers in natural product discovery and production

Green microbes: Potential solutions for key sustainable development goals

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