Harnessing shear stress preconditioning to improve cell viability in 3D post-printed biostructures using extrusion bioprinting

Boularaoui, Selwa Mokhtar; Shanti, Aya; Khan, Kamran A.; Iacoponi, Saverio; Christoforou, Nicolas; Stefanini, Cesare

doi:10.1016/j.bprint.2021.e00184

Cited by 7 publications

(4 citation statements)

References 49 publications

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“…[32][33][34][35][36][37][38][39] Furthermore, the gradual diffusion of crosslinkers through agarose molds of this technique reduces the stress exerted on cells caused by the mixing of crosslinkers with hydrogel precursors, a step commonly performed in traditional casting. [40] Another advantage lies in the porosity of agarose molds, which not only provides support for the cast implant during cell culture but also allow for the diffusion of gases and nutrients to the cells. This feature proves invaluable for casting delicate and soft hydrogels that may be easily damaged if transferred to a culture vessel without any support.…”

Section: Discussionmentioning

confidence: 99%

Biofabrication of Heterogeneous, Multi‐Layered, and Human‐Scale Tissue Transplants Using Eluting Mold Casting

Tosoratti,

Rütsche,

Asadikorayem

et al. 2023

Adv Funct Materials

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The creation of multi‐tissue auricular transplants for the treatment of microtia is a challenge due to the complex and layered structure of this anatomical tissue. A novel casting technique for the 3D biofabrication of heterogeneous, multi‐layered, and human‐scale tissue transplants using eluting agarose molds is presented. The molds are generated by casting agarose into custom 3D‐printed containers, termed metamolds, optimized to facilitate the hydrogel casting process based on geometric and topological constraints. Casting yields high resolution (50 µm) and allows for subsequent casting of further hydrogel layers on the transplant. Multi‐layered auricular constructs are fabricated on a cartilage core consisting of a hyaluronic acid‐alginate double network and an adjacent gelatin‐based dermal layer. Bonding between adjacent layers is achieved by orthogonal physical and enzymatic crosslinking of residual functional groups between each layer. Material composition and culture duration are optimized for each layer allowing for maturation into cartilaginous and pre‐vascularized dermal tissues. To demonstrate the scalability of this technique for the biofabrication of human‐sized transplants, bi‐layered human‐sized ears are cast. Overall, this novel casting technique offers a promising approach for the fabrication of complex tissue grafts, overcoming the limitations of other traditional biofabrication methods.

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Section: Discussionmentioning

confidence: 99%

Biofabrication of Heterogeneous, Multi‐Layered, and Human‐Scale Tissue Transplants Using Eluting Mold Casting

Tosoratti,

Rütsche,

Asadikorayem

et al. 2023

Adv Funct Materials

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“…Some methods have been developed to improve cell viability of MEB-bioprinted cell-laden constructs. For instance, moderate shear stress before bioprinting was found to enhance the ability of cells to tolerate the bioprinting induced stress and improve their viability post-bioprinting [ 86 ]. Li and coworkers used a bioprinting method that applied bioink pairs of alginate/MC and trisodium citrate (or GelMA) and achieved a cell viability of above 93% after bioprinting [ 87 , 88 ].…”

Section: D Bioprinting Technologiesmentioning

confidence: 99%

4D bioprinting of programmed dynamic tissues

Lai,

Liu,

et al. 2024

Bioactive Materials

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“…Extrusion printing forms cartilage scaffolds by extruding biomaterials and stacking them layer by layer according to a computer preset. In the practice of extrusion bioprinting cartilage scaffolds, this method has been found to build mechanically stronger and more accurate scaffolds and to promote chondrogenesis of seed cells seeded on the scaffold ( Boularaoui et al, 2022 ; Mueller et al, 2022 ). Li et al (2021) used silk fibroin and tyramine-substituted gelatin (SF-GT) to construct macro-porous hydrogel scaffolds by extrusion printing and compared the differentiation of seed cells seeded on the scaffolds via cell suspension and via cell aggregate.…”

Section: Technologies For Artificial Ac With Scaffoldmentioning

confidence: 99%

Novel advances in strategies and applications of artificial articular cartilage

Chen

Zhang²,

Zhang³

et al. 2022

Front. Bioeng. Biotechnol.

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Artificial articular cartilage (AC) is extensively applied in the repair and regeneration of cartilage which lacks self-regeneration capacity because of its avascular and low-cellularity nature. With advances in tissue engineering, bioengineering techniques for artificial AC construction have been increasing and maturing gradually. In this review, we elaborated on the advances of biological scaffold technologies in artificial AC including freeze-drying, electrospinning, 3D bioprinting and decellularized, and scaffold-free methods such as self-assembly and cell sheet. In the following, several successful applications of artificial AC built by scaffold and scaffold-free techniques are introduced to demonstrate the clinical application value of artificial AC.

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Harnessing shear stress preconditioning to improve cell viability in 3D post-printed biostructures using extrusion bioprinting

Cited by 7 publications

References 49 publications

Biofabrication of Heterogeneous, Multi‐Layered, and Human‐Scale Tissue Transplants Using Eluting Mold Casting

Biofabrication of Heterogeneous, Multi‐Layered, and Human‐Scale Tissue Transplants Using Eluting Mold Casting

4D bioprinting of programmed dynamic tissues

Novel advances in strategies and applications of artificial articular cartilage

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