Harnessing rhizosphere microbiomes for drought-resilient crop production

Vries, Franciska T. de; Griffiths, Robert I.; Knight, Christopher G.; Nicolitch, Océane; Williams, Alex

doi:10.1126/science.aaz5192

Cited by 477 publications

(322 citation statements)

References 66 publications

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“…Scientists have isolated rhizospheric and endophytic bacteria and fungi from arid regions or from xerophytes, and these microbes have been shown to improve the growth of rice, sorghum, corn, cabbage, millet, and wheat under drought-stress conditions [ 14 , 80 , 81 , 82 , 83 , 84 ]. There is abundant evidence that root-associated microbes can help sustain plant growth under drought conditions [ 12 , 18 ]. However, no systematic study on the identity and PGP potential of upland rice root-associated microbes as well as their effects on drought tolerance exists.…”

Section: Discussionmentioning

confidence: 99%

“…A great variety of microbes are found to inhabit plant surfaces and different plant tissues and organs, including roots, rhizosphere, phyllosphere, stems, leaves, flowers, and seeds [ 7 , 8 , 9 ]. Root-associated microbes can improve host plant growth (such as N fixation [ 10 ], phosphate solubilization [ 11 ] and iron chelation), suppress pathogens, and mobilize some micronutrients, and they offer the potential to increase crop plant resilience to future drought [ 12 ]. Microbial-based plant biotechnology has been proven to be more effective than plant breeding and genetic improvement methods [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Microbial Diversity of Upland Rice Roots and Their Influence on Rice Growth and Drought Tolerance

Pang

Zhao

et al. 2020

Microorganisms

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Among abiotic stresses, drought is one of the most important factors limiting plant growth. To increase their drought tolerance and survival, most plants interact directly with a variety of microbes. Upland rice (Oryza sativa L.) is a rice ecotype that differs from irrigated ecotype rice; it is adapted to both drought-stress and aerobic conditions. However, its root microbial resources have not been explored. We isolated bacteria and fungi from roots of upland rice in Xishuangbanna, China. Four hundred sixty-two endophytic and rhizospheric isolates (337 bacteria and 125 fungi) were distributed. They were distributed among 43 genera on the basis of 16S rRNA and internal transcribed spacer (ITS) gene sequence analysis. Notably, these root microbes differed from irrigated rice root microbes in irrigated environments; for example, members of the Firmicutes phylum were enriched (by 28.54%) in the roots of the upland plants. The plant growth-promoting (PGP) potential of 217 isolates was investigated in vitro. The PGP ability of 17 endophytic and 10 rhizospheric isolates from upland rice roots was evaluated under well-irrigated and drought-stress conditions, and 9 fungal strains increased rice seedling shoot length, shoot and root fresh weight (FW), antioxidant capability, and proline (Pro) and soluble sugar contents. Our work suggests that fungi from upland rice roots can increase plant growth under irrigated and drought-stress conditions and can serve as effective microbial resources for sustainable agricultural production in arid regions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microbial Diversity of Upland Rice Roots and Their Influence on Rice Growth and Drought Tolerance

Pang

Zhao

et al. 2020

Microorganisms

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“…Increasingly the use of plant-associated microorganisms, especially rhizosphere-colonizing and endophytic bacteria and fungi, is being studied as a rapid and cost-effective strategy to augment the development of stress tolerant crop varieties ( Nadeem et al, 2014 ; Shrivastava and Kumar, 2015 ; Numan et al, 2018 ). It is well established that phytobiome members, especially rhizosphere and root dwellers, can play important roles in mediating abiotic stresses tolerance via promotion of plant growth, enhancement of nutrient availability, disease control, and modulation of plant abiotic stress signaling and response pathways ( Yang et al, 2009 ; Hardoim et al, 2015 ; Reinhold-Hurek et al, 2015 ; de Vries et al, 2020 ). With regard to salinity, previous studies focused on plant growth-promoting rhizobacteria (PGPR) showed that they may alleviate salinity induced osmotic stress, nutrient deficiency, oxidative stress, and ion toxicity via the production of bioactive compounds and modifications to the rhizosphere environment.…”

Section: Introductionmentioning

confidence: 99%

Phenazine-Producing Rhizobacteria Promote Plant Growth and Reduce Redox and Osmotic Stress in Wheat Seedlings Under Saline Conditions

et al. 2020

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Application of plant growth promoting bacteria may induce plant salt stress tolerance, however the underpinning microbial and plant mechanisms remain poorly understood. In the present study, the specific role of phenazine production by rhizosphere-colonizing Pseudomonas in mediating the inhibitory effects of salinity on wheat seed germination and seedling growth in four different varieties was investigated using Pseudomonas chlororaphis 30-84 (wild type) and isogenic derivatives deficient or enhanced in phenazine production. The results showed that varieties differed in how they responded to the salt stress treatment and the benefits derived from colonization by P. chlororaphis 30-84. In all varieties, the salt stress treatment significantly reduced seed germination, and in seedlings, reduced relative water content, increased reactive oxygen species (ROS) levels in leaves, and in three of four varieties, reduced shoot and root production compared to the no salt stress treatment. Inoculation of seeds with Pseudomonas chlororaphis 30-84 wild type or derivatives promoted salt-stress tolerance in seedlings of the four commercial winter wheat varieties tested, but the salt-stress tolerance phenotype was not entirely due to phenazine production. For example, all P. chlororaphis derivatives (including the phenazine-producing mutant) significantly improved relative water content in two varieties, Iba and CV 1, for which the salt stress treatment had a large impact. Importantly, all P. chlororaphis derivatives enabled the salt inhibited wheat varieties studied to maintain above ground productivity in saline conditions. However, only phenazine-producing derivatives enhanced the shoot or root growth of seedlings of all varieties under nonsaline conditions. Notably, ROS accumulation was reduced, and antioxidant enzyme (catalase) activity enhanced in the leaves of seedlings grown in saline conditions that were seed-treated with phenazine-producing P. chlororaphis derivatives as compared to noninoculated seedlings. The results demonstrate the capacity of P. chlororaphis to improve salt tolerance in wheat seedlings by promoting plant growth and reducing osmotic stress and a role for bacterial phenazine production in reducing redox stress.

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“…The effects of drought stress on plants are mainly reflected in the effects on cell activity and organ and tissue function [ 1 , 2 ]. A decrease in the water content will result in the inhibition of photosynthesis, the slow growth of plants, and influence of the biomass or yield [ 3 ]. After drought stress, the metabolic process of cells is blocked, which leads to the accumulation of H 2 O 2 , 1 O 2 , and O 2 • − in cells [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Ability to Control Water Loss in the Detached Leaves of Wedelia trilobata, Wedelia chinensis, and Their Hybrid

Zhang

Chen

Huang

et al. 2020

Plants

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In the process of biological invasion, hybridization between invasive species and native species is very common, which may lead to the formation of hybrids with a stronger adaptability. The hybrid of Wedelia trilobata (an alien invasive species) and Wedelia chinensis (an indigenous congener) has been found in South China. In our previous study, we found that the hybrid showed heterosis under cadmium stress. However, the results of this experiment demonstrated that the leaves of the hybrid had no heterosis in controlling water loss. The results showed that the water loss rate of W. trilobata was the slowest, that of W. chinensis was the fastest, and that of the hybrid was in the middle. Compared with W. chinensis and the hybrid, W. trilobata accumulated more abscisic acid (ABA) in leaves to control water loss. After the leaves were detached, W. chinensis leaves suffered the most serious damage, the lowest maximum photochemical efficiency, the most serious membrane lipid peroxidation, and the largest accumulation of malondialdehyde and reactive oxygen species. Compared with W. chinensis and its hybrid, the leaves of W. trilobata could accumulate more antioxidant enzymes and antioxidants, and the total antioxidant capacity was the strongest. The results demonstrate that the ability of the hybrid to reduce water loss was lower than that of W. trilobata, but higher than that of W. chinensis. They showed that the drought resistance of the hybrid may be higher than that of W. chinensis, and it might threaten the survival of W. chinensis.

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Harnessing rhizosphere microbiomes for drought-resilient crop production

Cited by 477 publications

References 66 publications

Microbial Diversity of Upland Rice Roots and Their Influence on Rice Growth and Drought Tolerance

Microbial Diversity of Upland Rice Roots and Their Influence on Rice Growth and Drought Tolerance

Phenazine-Producing Rhizobacteria Promote Plant Growth and Reduce Redox and Osmotic Stress in Wheat Seedlings Under Saline Conditions

Comparison of the Ability to Control Water Loss in the Detached Leaves of Wedelia trilobata, Wedelia chinensis, and Their Hybrid

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