Harnessing electrospun nanofibers to recapitulate hierarchical fibrous structures of meniscus

Wang, Xiaoyu; Zhu, Jingjing; Sun, Binbin; Jin, Quan; Li, Haiyan; Xia, Changlei; Wang, Hongsheng; Mo, Xiumei; Wu, Jinglei

doi:10.1002/jbm.b.34692

Cited by 24 publications

(26 citation statements)

References 49 publications

(146 reference statements)

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“…It was found that the crystallinity of C-CNF was higher than that of W-CNF due to the higher crystal structure of cotton [16,17], which were consistent with the results of SEM analysis in Section 3.1. The crystallinity of W-CNF was higher than that of MCC, and the crystallinity of MCC was higher than that of WC [18,19]. Different calculation methods of these three could result in different crystallinity calculation results, and perfect single crystal was extremely difficult to obtain.…”

Section: Crystallinity From Xrd Analysismentioning

confidence: 98%

“…The research on corn, wheat, shell, and reed in the terahertz frequency spectrum characteristics was at 0.2-1.8 THz. The results showed that the using of the terahertz wave technology for plant cellulose detection could achieve quick judgment of cellulose in biomass materials [16][17][18]. Luo et al [19] calculated 0.2-1.0 THz spectrum absorption coefficient and refractive index.…”

Section: Introductionmentioning

confidence: 99%

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Novel Terahertz Spectroscopy Technology for Crystallinity and Crystal Structure Analysis of Cellulose

et al. 2020

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Crystallinity is an essential indicator for evaluating the quality of fiber materials. Terahertz spectroscopy technology has excellent penetrability, no harmful substances, and commendable detection capability of absorption characteristics. The terahertz spectroscopy technology has great application potential in the field of fiber material research, especially for the characterization of the crystallinity of cellulose. In this work, the absorption peak of wood cellulose, microcrystalline cellulose, wood nano cellulose, and cotton nano cellulose were probed in the terahertz band to calculate the crystallinity, and the result compared with XRD and FT-IR analysis. The vibration model of cellulose molecular motion was obtained by density functional theory. The results showed that the average length of wood cellulose (WC) single fiber was 300 μm. The microcrystalline cellulose (MCC) was bar-like, and the average length was 20 μm. The cotton cellulose nanofiber (C-CNF) was a single fibrous substance with a length of 50 μm, while the wood cellulose nanofiber (W-CNF) was with a length of 250 μm. The crystallinity of cellulose samples in THz was calculated as follows: 73% for WC, 78% for MCC, 85% for W-CNF, and 90% for C-CNF. The crystallinity values were obtained by the three methods which were different to some extent. The absorption peak of the terahertz spectra was most obvious when the samples thickness was 1 mm and mixed mass ratio of the polyethylene and cellulose was 1:1. The degree of crystallinity was proportional to the terahertz absorption coefficients of cellulose, the five-movement models of cellulose molecules corresponded to the five absorption peak positions of cellulose.

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Section: Crystallinity From Xrd Analysismentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Novel Terahertz Spectroscopy Technology for Crystallinity and Crystal Structure Analysis of Cellulose

et al. 2020

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“…[59][60][61][62][63][64] Recently, we adopted this technique to fabricate an electrospun nanofiber yarn scaffold, where rabbit meniscus cells infiltrated the full depth of scaffolds within 3 weeks. 54 A considerable increase in the population of cells with a uniform distribution of meniscus cells within the nanofiber yarn scaffold was observed after 5 weeks. Moreover, meniscus cells secreted extracellular matrix products within the nanofiber yarn scaffold, which profoundly improves the tensile strength of scaffolds with respect to the unseeded scaffolds.…”

Section: Enhancing Cell Infiltration Into Electrospun Scaffoldsmentioning

confidence: 94%

“…Therefore, we tested this hypothesis in a recent study by recreating the circumferential collagen fibril bundles via electrospinning with a dynamic liquid collection system. 54 We electrospun poly(L-lactic acid-cocaprolactone) (PLLA-CL)/decellularized meniscus extracellular matrix (dmECM) solution to prepare electrospun nanofibrous yarn scaffolds (Figure 3A). In addition, we also prepare aligned and random nanofibrous scaffolds via electrospinning.…”

Section: Similarities Between Meniscal Fibrous Structure and Electros...mentioning

confidence: 99%

Advances in electrospun scaffolds for meniscus tissue engineering and regeneration

Wang

Ding

et al. 2021

J Biomed Mater Res

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The meniscus plays a critical role in maintaining the homeostasis, biomechanics, and structural stability of the knee joint. Unfortunately, it is predisposed to damages either from sports-related trauma or age-related degeneration. The meniscus has an inherently limited capacity for tissue regeneration. Self-healing of injured adult menisci only occurs in the peripheral vascularized portion, while the spontaneous repair of the inner avascular region seems never happens. Repair, replacement, and regeneration of menisci through tissue engineering strategies are promising to address this problem. Recently, many scaffolds for meniscus tissue engineering have been proposed for both experimental and preclinical investigations. Electrospinning is a feasible and versatile technique to produce nano-to micro-scale fibers that mimic the microarchitecture of native extracellular matrix and is an effective approach to prepare nanofibrous scaffolds for constructing engineered meniscus. Electrospun scaffolds are reported to be capable of inducing colonization of meniscus cells by modulating local extracellular density and stimulating endogenous regeneration by driving reprogramming of meniscus wound microenvironment. Electrospun nanofibrous scaffolds with tunable mechanical properties, controllable anisotropy, and various porosities have shown promises for meniscus repair and regeneration and will undoubtedly inspire more efforts in exploring effective therapeutic approaches towards clinical applications. In this article, we review the current advances in the use of electrospun nanofibrous scaffolds for meniscus tissue engineering and repair and discuss prospects for future studies.

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“…fixed macrophage-seeded GT/PCL membranes (14 mm diameter) were embedded in paraffin at days 2, 4, and 6. Sections were stained with hematoxylin and eosin (H&E) (Wang et al, 2021).…”

Section: Paraformaldehydementioning

confidence: 99%

Regeneration of Subcutaneous Cartilage in a Swine Model Using Autologous Auricular Chondrocytes and Electrospun Nanofiber Membranes Under Conditions of Varying Gelatin/PCL Ratios

Zheng

Wang

Xue

et al. 2021

Front. Bioeng. Biotechnol.

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The scarcity of ideal biocompatible scaffolds makes the regeneration of cartilage in the subcutaneous environment of large animals difficult. We have previously reported the successful regeneration of good-quality cartilage in a nude mouse model using the electrospun gelatin/polycaprolactone (GT/PCL) nanofiber membranes. The GT/PCL ratios were varied to generate different sets of membranes to conduct the experiments. However, it is unknown whether these GT/PCL membranes can support the process of cartilage regeneration in an immunocompetent large animal model. We seeded swine auricular chondrocytes onto different GT/PCL nanofiber membranes (GT:PCL = 30:70, 50:50, and 70:30) under the sandwich cell-seeding mode. Prior to subcutaneously implanting the samples into an autologous host, they were cultured in vitro over a period of 2 weeks. The results revealed that the nanofiber membranes with different GT/PCL ratios could support the process of subcutaneous cartilage regeneration in an autologous swine model. The maximum extent of homogeneity in the cartilage tissues was achieved when the G5P5 (GT: PC = 50: 50) group was used for the regeneration of cartilage. The formed homogeneous cartilage tissues were characterized by the maximum cartilage formation ratio. The extents of the ingrowth of the fibrous tissues realized and the extents of infiltration of inflammatory cells achieved were found to be the minimum in this case. Quantitative analyses were conducted to determine the wet weight, cartilage-specific extracellular matrix content, and Young’s modulus. The results indicated that the optimal extent of cartilage formation was observed in the G5P5 group. These results indicated that the GT/PCL nanofiber membranes could serve as a potential scaffold for supporting subcutaneous cartilage regeneration under clinical settings. An optimum GT/PCL ratio can promote cartilage formation.

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Harnessing electrospun nanofibers to recapitulate hierarchical fibrous structures of meniscus

Cited by 24 publications

References 49 publications

Novel Terahertz Spectroscopy Technology for Crystallinity and Crystal Structure Analysis of Cellulose

Novel Terahertz Spectroscopy Technology for Crystallinity and Crystal Structure Analysis of Cellulose

Advances in electrospun scaffolds for meniscus tissue engineering and regeneration

Regeneration of Subcutaneous Cartilage in a Swine Model Using Autologous Auricular Chondrocytes and Electrospun Nanofiber Membranes Under Conditions of Varying Gelatin/PCL Ratios

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