Harnessing aptamers for electrochemical detection of endotoxin

Kim, Sang Hoon; Su, Wenqiong; Cho, MiSuk; Lee, Youngkwan; Choe, Wonyoung

doi:10.1016/j.ab.2012.02.016

Cited by 111 publications

(68 citation statements)

References 39 publications

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“…[16][17][18] To address these drawbacks, scholars have developed alternative methods for LPS detection by using various signals, such as colorimetric, [19][20][21][22] surface-enhanced Raman scattering, 23 uorescence, 24 and electrochemical signals. [25][26][27] These methods exhibit high sensitivity and accuracy. However, with increasing concerns on food safety, a large number of food samples should be efficiently tested for LPS because bacteria are commonly found in numerous raw food samples.…”

Section: 15mentioning

confidence: 99%

An aptamer-based fluorescence probe for facile detection of lipopolysaccharide in drinks

et al. 2017

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Bacterial toxin contamination is a serious food safety issue. Rapid, facile detection of bacterial toxins in food is crucial to control their harmful effects on human health. In this study, a facile, selective, and cost-effective method with a 6-carboxy-X-rhodamine labeled lipopolysaccharide binding aptamer (ROX-LBA)/GO fluorescent probe was developed based on the specific recognition characteristics of aptamers and the super fluorescence quenching efficiency of graphene oxide (GO) and used for the detection of lipopolysaccharides (LPS). ROX-LBA is adsorbed on the GO surface because of its strong p-p stacking interactions in the flexible single-stranded state, and fluorescence is quenched by GO via Förster resonance energy transfer (FRET). After adding LPS, the aptamer binds to LPS and forms a LPS/ROX-LBA complex, which exhibits weak binding affinity with GO. ROX-LBA cannot effectively combine with GO, and fluorescence is restored. The concentration of LPS can be quantitatively analyzed by observing fluorescence changes in the sensing system. The fluorescence intensity proportionally increases with LPS concentration (25-1600 ng mL À1 ), and the detection limit is 15.7 ng mL À1 . Hence, the proposed method provides an alternative strategy for the specific detection of endotoxin in drink samples (e.g., apple juice and beer) and could be widely applied to quantify various bacterial toxins by replacing the aptamer sequences.

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Section: 15mentioning

confidence: 99%

An aptamer-based fluorescence probe for facile detection of lipopolysaccharide in drinks

et al. 2017

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“…Bacterial endotoxins are also one of the major contaminants present in pharmaceutical products, causing sever septic shock in humans and animals (Magalhaes et al, 2007). Kim et al fabricated an electrochemical aptasensors for the detection of endotoxins from crude biological liquor (Kim et al, 2012). Seeds of the leguminous herb lupin are the widely used source of low cost protein.…”

Section: Electrochemical Aptasensor For Environmental Monitoringmentioning

confidence: 99%

Aptamer based electrochemical sensors for emerging environmental pollutants

2014

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Environmental contaminants monitoring is one of the key issues in understanding and managing hazards to human health and ecosystems. In this context, aptamer based electrochemical sensors have achieved intense significance because of their capability to resolve a potentially large number of problems and challenges in environmental contamination. An aptasensor is a compact analytical device incorporating an aptamer (oligonulceotide) as the sensing element either integrated within or intimately associated with a physiochemical transducer surface. Nucleic acid is well known for the function of carrying and passing genetic information, however, it has found a key role in analytical monitoring during recent years. Aptamer based sensors represent a novelty in environmental analytical science and there are great expectations for their promising performance as alternative to conventional analytical tools. This review paper focuses on the recent advances in the development of aptamer based electrochemical sensors for environmental applications with special emphasis on emerging pollutants.

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“…As a new platform technology, aptamer-based techniques bioassay has recently been adopted in food analysis, clinical, medical and environmental monitoring [80][81][82][83][84][85]. In food safety monitoring, aptamers are applied for detection of chemical and/or biological hazards.…”

Section: Application Of Aptamer In Food Safetymentioning

confidence: 99%

Current Advances and Prospects on Implementation of Highly Sensitive Aptamer-based Dual System for Melamine Detection: New Promising Tool of Great Affinity

Ndikubwimana¹

2015

J Bioprocess Biotech

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Harnessing aptamers for electrochemical detection of endotoxin

Cited by 111 publications

References 39 publications

An aptamer-based fluorescence probe for facile detection of lipopolysaccharide in drinks

An aptamer-based fluorescence probe for facile detection of lipopolysaccharide in drinks

Aptamer based electrochemical sensors for emerging environmental pollutants

Current Advances and Prospects on Implementation of Highly Sensitive Aptamer-based Dual System for Melamine Detection: New Promising Tool of Great Affinity

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