Harmonized Collaborative Validation of Aflatoxins and Sterigmatocystin in White Rice and Sorghum by Liquid Chromatography Coupled to Tandem Mass Spectrometry

Abstract

An interlaboratory study was performed in eight laboratories to validate a liquid chromatography–tandem mass spectrometry (LC/MS/MS) method for the simultaneous determination of aflatoxins and sterigmatocystin (STC) in white rice and sorghum (Sorghum bicolor). Fortified samples (at three different levels) of white rice and sorghum were extracted, purified through a solid-phase extraction (SPE) column, and then analyzed by LC/MS/MS. The apparent recoveries (ARs) ranged from 78.8% to 95.0% for aflatoxins and from 85.3% to 96.7% for STC. The relative standard deviations for repeatability (RSDr) and reproducibility (RSDR) of aflatoxins were in the ranges 7.9%–33.8% and 24.4%–81.0%, respectively. For STC, the RSDr ranged from 7.1% to 40.2% and the RSDR ranged from 28.1% to 99.2%. The Horwitz ratio values for the aflatoxins and STC ranged from 0.4 to 1.2 in white rice and from 0.3 to 1.0 in sorghum, respectively. These results validated this method for the simultaneous determination of aflatoxins and STC by LC/MS/MS after SPE column cleanup. The percentages of satisfactory Z-score values (|Z| ≤ 2) were the following: for white rice, 100% for aflatoxins and STC; for sorghum, 100%, except in data from two laboratories for STC (0.3 μg/kg). This validated that the LC/MS/MS method was successfully applied for the determination of aflatoxins and STC in 20 white rice and 20 sorghum samples sourced from Korean markets.

A Focus on Aflatoxin in Feedstuffs: New Developments in Analysis and Detection, Feed Composition Affecting Toxin Contamination, and Interdisciplinary Approaches to Mitigate It

“…For a thorough review of chromatographic and spectrometric techniques used for mycotoxin analysis, we suggest the paper wrote by Li and coworkers [97]. Ok and coworkers recently opted to include aflatoxins and sterigmatocystin in the same analysis using tandem MS for their assay in sorghum and rice [98].…”

Fabrication of an activatable hybrid persistent luminescence nanoprobe for background-free bioimaging-guided investigation of food-borne aflatoxin in vivo

“…Aatoxins, which are secondary metabolites mainly produced by the fungi of A. parasiticus and Aspergillus avus, are known to be highly carcinogenic, hepatotoxic, and mutagenic food-borne toxins. 31,32 As the most common aatoxin, AFT B1 is of great food-safety concern and research interest and hence, in vivo probing of AFT B1 digested in the living body would be of great signicance. 33,34 In the present FRET assay, persistent luminescence nanophosphors with the distinct advantages of improved sensitivity and signal-to-noise ratio in in vivo bioimaging as well as excellent photostability and biocompatibility, were employed as the photoluminescence nanoprobes, while copper sulde nanoparticles (CuS) were utilized as the quencher.…”

Development and in-house validation of a rapid and simple to use ELISA for the detection and measurement of the mycotoxin sterigmatocystin

“…STG can be detected using thin layer chromatography [9] or HPLC [12]. LC-MS/MS methods have also been developed for the detection of STG in food and feed such as wheat, rice, oat, rye, maize and barley [13]; white rice and sorghum [14]; beer and cheese [12]; feed [15]. STG is also commonly included in many LC-MS/MS multi-mycotoxin methods [16][17][18][19][20].…”