Harmine stimulates neurogenesis of human neural cells <i>in vitro</i>

Dakic, Vanja; Maciel, Roseli Martins Tristão; Drummond, Hannah; Nascimento, Juliana; Trindade, Pablo; Rehen, Stevens

doi:10.7287/peerj.preprints.1957

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…Its head-to-head comparison with psilocybin and ketamine has yet to be performed, but it is clear that ayahuasca is a major contestant for the most potent psychedelic antidepressant. The main chemical components present in the brew, harmine and N,Ndimethyltryptamine (N,N-DMT), as well as the related compound 5-MeO DMT, present in the secretion of the toad Bufo alvarius and in the yopo plant snuff, have been demonstrated to induce several cellular changes related to the formation of new synapses and even new neurons entirely, as shown by the research groups of Stevens Rehen (Dakic et al, 2016;Dakic et al, 2017), Richardson Leão (Lima da Cruz et al, 2018), and David Olson (Ly et al 2018. Congruent results were recently seen for d-LSD by a consortium of my laboratory with those of Stevens Rehen, Draulio de Araujo, Luis Fernando Tófoli, and Daniel Martins-de-Souza, with support from the Beckley Foundation (Cini et al, 2019).…”

Section: Prefacementioning

confidence: 99%

Ayahuasca Healing and Science

2021

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Section: Prefacementioning

confidence: 99%

Ayahuasca Healing and Science

2021

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Proteomic changes induced by harmine in human brain organoids reveal signaling pathways related to neuroprotection

Karmirian

Goto‐Silva

et al. 2021

Preprint

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Harmine is a β-carboline found in Banisteriopsis caapi, a constituent of ayahuasca brew. Ayahuasca is consumed as a beverage in native Americans' sacred rituals and in religious ceremonies in Brazil. Throughout the years, the beneficial effects of ayahuasca to improve mental health and life quality have been reported, which propelled the investigation of its therapeutic potential to target neurological disorders such as depression and anxiety. Indeed, antidepressant effects of ayahuasca have been described, raising the question of which cellular mechanisms might underlie those effects. Previous animal studies describe potential neuroprotective mechanisms of harmine, including anti-inflammatory and antioxidant activities, and neurotrophin signaling activation. However, the cellular and molecular mechanisms modulated by harmine in human models remain less investigated. Here we analyzed the short-term changes in the proteome of human brain organoids treated with harmine using shotgun mass spectrometry. Harmine upregulates proteins related to synaptic vesicle cycle, cytoskeleton-dependent intracellular transport, cell cycle, glucose transporter-4 translocation, and neurotrophin signaling pathway. In addition, protein expression levels of Akt and phosphorylated CREB were increased after 24 hour-treatment. Our results shed light on the potential mechanisms that may underlie harmine-induced neuroprotective effects.

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Harmine stimulates neurogenesis of human neural cells in vitro

Abstract: Harmine is a β-carboline alkaloid present at highest concentration in the psychotropic plant decoction Ayahuasca. In rodents, classical antidepressants

Cited by 2 publications

References 15 publications

Ayahuasca Healing and Science

Ayahuasca Healing and Science

Proteomic changes induced by harmine in human brain organoids reveal signaling pathways related to neuroprotection

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