Harmine induces apoptosis and inhibits tumor cell proliferation, migration and invasion through down-regulation of cyclooxygenase-2 expression in gastric cancer

Zhang, Hao; Sun, Kai; Jing, Ding; Xu, Huae; Zhu, Lingjun; Zhang, Kai; Li, Xin; Sun, Wei

doi:10.1016/j.phymed.2013.09.007

Cited by 52 publications

(45 citation statements)

References 39 publications

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“…A previous study reported that the use of Traditional Chinese Medicine against invasion and metastasis in cancer is increasingly popular. Numerous studies have demonstrated that certain Traditional Chinese Medicines and their extracts may effectively inhibit tumor invasion and metastasis (5,6).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, it is important to explore a novel methods of enhancing the anticancer effect of PTX and to elucidate novel therapies that may reverse the resistance to PTX in cancer patients. Harmine (HM), a tricyclic compound belonging to the β-carboline alkaloids, has been demonstrated to inhibit migration and invasion in gastric cancer in vitro (6). However, the synergistic antitumor effect of a combined PTX and HM treatment on migration and invasion in human gastric cancer cells remained to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

“…COX-2 overexpression was reported to be involved in the genesis and development of gastric cancer and was suggested to be closely associated with the invasiveness and metastasis of gastric cancer (6). It has been well-established that prostaglandins are synthesized from arachidonate under the catalytic effects of COX-2 and these prostaglandins may enhance the activity of several MMPs, strengthen CD44 expression and reduce the expression of epithelial cadherin, resulting in the reinforced invasiveness of tumors (18)(19)(20).…”

Section: Discussionmentioning

confidence: 99%

“…Harmine (HM), a tricyclic compound found in Peganum harmala and belonging to the β-carboline alkaloids, has been proven to possess antitumor properties and was previously examined for its potential use for cancer therapy (4,5). It was reported that HM effectively inhibited tumor migration and invasion in vitro (6). However, the synergistic antitumor effect of a combination of PTX and HM on migration and invasion of human gastric cancer cells remains to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

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Paclitaxel combined with harmine inhibits the migration and invasion of gastric cancer cells through downregulation of cyclooxygenase-2 expression

2015

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Abstract. Cyclooxygenase-2 (COX-2) has a critical role in the invasiveness and metastasis of gastric cancer. In addition, paclitaxel (PTX) and harmine (HM) were reported to be potential therapeutic drug candidates for cancer therapy; however, the synergistic antitumor effect of PTX and HM combined treatment on the human gastric cancer cells remains to be elucidated. The aim of the present study was to evaluate the effects of PTX and/or HM on the cell migration and invasion in two human gastric cancer cell lines, SGC-7901 and MKN-45. MTT assay was used to detect the growth inhibition induced by PTX and HM . The Transwell assay was employed to assess the effects of PTX and HM on the cell migration and invasion. The expression levels of COX-2 and matrix metalloproteinase-9 (MMP-9) were analyzed by western blot analysis. The results demonstrated that PTX and HM inhibited cell proliferation in a dose-dependent manner. Individually PTX and HM were able to inhibit the migration and invasion of two human gastric cancer cells; however, the combination of PTX and HM exerted synergistic effects on migration and invasion inhibition, with downregulation of COX-2 and matrix metalloproteinase (MMP)-9. In conclusion, the results of the present study indicated that combination chemotherapy using PTX with HM exerted an antitumor effect, which may be implicated for the treatment of gastric cancer. Of note, the combination of the two drugs inhibited migration and invasion more effectively compared with each drug alone, the mechanism of which proceeded via the downregulation of COX-2 expression.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Paclitaxel combined with harmine inhibits the migration and invasion of gastric cancer cells through downregulation of cyclooxygenase-2 expression

2015

Self Cite

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“…It has been found that the MMP9 15 , COX2 16 , c-Myc 17 genes are upregulated, and the CDKN2A (formerly p16) 18 and TP53 19 genes are downregulated. Here, we focused on changes in appl1 expression in gc and the relationships of appl1 and associated clinicopathologic characteristics with clinical prognosis.…”

Section: Discussionmentioning

confidence: 99%

Expression of APPL1 Is Correlated with Clinicopathologic Characteristics and Poor prognosis in Patients with Gastric Cancer

Zhai¹,

Song²,

Zhu³

et al. 2016

Current Oncology

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A new potential anti‐cancer beta‐carboline derivative decreases the expression levels of key proteins involved in glioma aggressiveness: A proteomic investigation

Carvalho

Viaene

Vandenbussche

et al. 2019

Drug Development Research

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Gliomas remain highly fatal due to their high resistance to current therapies. Deregulation of protein synthesis contributes to cancer onset and progression and is a source of rising interest for new drugs. CM16, a harmine derivative with predicted high blood–brain barrier penetration, exerts antiproliferative effects partly through translation inhibition. We evaluated herein how CM16 alters the proteome of glioma cells. The analysis of the gel‐free LC/MS and auto‐MS/MS data showed that CM16 induces time‐ and concentration‐dependent significant changes in the total ion current chromatograms. In addition, we observed spontaneous clustering of the samples according to their treatment condition and their proper classification by unsupervised and supervised analyses, respectively. A two‐dimensional gel‐based approach analysis allowed us to identify that treatment with CM16 may downregulate four key proteins involved in glioma aggressiveness and associated with poor patient survival (HspB1, BTF3, PGAM1, and cofilin), while it may upregulate galectin‐1 and Ebp1. Consistently with the protein synthesis inhibition properties of CM16, HspB1, Ebp1, and BTF3 exert known roles in protein synthesis. In conclusion, the downregulation of HspB1, BTF3, PGAM1 and cofilin bring new insights in CM16 antiproliferative effects, further supporting CM16 as an interesting protein synthesis inhibitor to combat glioma.

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Harmine induces apoptosis and inhibits tumor cell proliferation, migration and invasion through down-regulation of cyclooxygenase-2 expression in gastric cancer

Cited by 52 publications

References 39 publications

Paclitaxel combined with harmine inhibits the migration and invasion of gastric cancer cells through downregulation of cyclooxygenase-2 expression

Paclitaxel combined with harmine inhibits the migration and invasion of gastric cancer cells through downregulation of cyclooxygenase-2 expression

Expression of APPL1 Is Correlated with Clinicopathologic Characteristics and Poor prognosis in Patients with Gastric Cancer

A new potential anti‐cancer beta‐carboline derivative decreases the expression levels of key proteins involved in glioma aggressiveness: A proteomic investigation

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