Harmful algal bloom toxins alter c-Fos protein expression in the brain of killifish, Fundulus heteroclitus

Salierno, James D.; Snyder, Natalie; Murphy, Anne Z.; Poli, Mark; Hall, Sherwood; Baden, Daniel G.; Kane, Andrew S.

doi:10.1016/j.aquatox.2006.04.010

Cited by 36 publications

(29 citation statements)

References 32 publications

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“…The majority of work in killifish has examined handling stress. This stressor has been shown to alter neuronal activation in the killifish brain, as demonstrated by increased expression of c-fos in various brain regions (Salierno et al, 2006), and to increase plasma cortisol, which is the primary glucocorticoid in fish (Leach and Taylor, 1980). The northern and southern subspecies of killifish differ in their susceptibility to handling stress.…”

Section: Behavioural and Biotic Stressorsmentioning

confidence: 99%

What is environmental stress? Insights from fish living in a variable environment

Schulte

2014

Journal of Experimental Biology

258

165

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Although the term environmental stress is used across multiple fields in biology, the inherent ambiguity associated with its definition has caused confusion when attempting to understand organismal responses to environmental change. Here I provide a brief summary of existing definitions of the term stress, and the related concepts of homeostasis and allostasis, and attempt to unify them to develop a general framework for understanding how organisms respond to environmental stressors. I suggest that viewing stressors as environmental changes that cause reductions in performance or fitness provides the broadest and most useful conception of the phenomenon of stress. I examine this framework in the context of animals that have evolved in highly variable environments, using the Atlantic killifish, Fundulus heteroclitus, as a case study. Consistent with the extreme environmental variation that they experience in their salt marsh habitats, killifish have substantial capacity for both short-term resistance and long-term plasticity in the face of changing temperature, salinity and oxygenation. There is inter-population variation in the sensitivity of killifish to environmental stressors, and in their ability to acclimate, suggesting that local adaptation can shape the stress response even in organisms that are broadly tolerant and highly plastic. Whole-organism differences between populations in stressor sensitivity and phenotypic plasticity are reflected at the biochemical and molecular levels in killifish, emphasizing the integrative nature of the response to environmental stressors. Examination of this empirical example highlights the utility of using an evolutionary perspective on stressors, stress and stress responses.

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Section: Behavioural and Biotic Stressorsmentioning

confidence: 99%

What is environmental stress? Insights from fish living in a variable environment

Schulte

2014

Journal of Experimental Biology

258

165

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“…The neurological effect of harmful algal bloom toxins on the killifish Fundulus heteroclitus has been investigated (Salierno et al, 2006). In this work, induction of c-Fos, a protein biomarker for neuronal and regional brain activity, was evaluated when specimens were exposed to the excitatory neurotoxins domoic acid and brevetoxin B, and the paralytic neurotoxin saxitoxin.…”

Section: Consumer Neuroecologymentioning

confidence: 99%

Chemical Defenses: From Compounds to Communities

Paul

Arthur

Ritson‐Williams

et al. 2007

The Biological Bulletin

176

152

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“…In rainbow trout brain, the administration of kainic acid increased c-Fos mRNA expression after 60 min and for up to 120 min, at which time it then decreased towards control levels (Matsuoka et al 1998). Another study described an increased c-Fos expression in brainstem of rainbow trout following a startle response (Bosch et al 2001), and recently Salierno et al (2006) found altered c-Fos protein expression in killifish which were exposed to harmful algal bloom toxins. However, no studies to date have investigated alterations in c-Fos mRNA or protein expression in brain of fish subjected to hypercapnic stress.…”

Section: Time Points Testedmentioning

confidence: 98%

Molecular cloning and gene expression analysis in aquaculture science: a review focusing on respiration and immune responses in European sea bass (Dicentrarchus labrax)

Terova

Rimoldi

Parisi

et al. 2012

Rev Fish Biol Fisheries

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Although fish farming has been practiced for 4,000 years, aquaculture research dates back only to about 1,870, whereas molecular techniques, in addition to the more traditional methods of biotechnology, were introduced only recently. Contemporary genomic approaches (often adapted from human or medical research) such as cDNA cloning and sequencing, cDNA microarray/expression analysis, and functional genomics, combined with improvements in transgenic technologies, have enhanced possibilities for aquacultural biotechnologists for improving fish growth rates and increasing resistance to pathogens and stressors.Although genomic technologies in fish have been applied primarily to model organisms, such as zebrafish (Danio rerio), fugu or pufferfish (Tetraodon nigroviridis), and medaka (Oryzias latipes), many teleosts of interest for biological research and with potential application in aquaculture have unique physiological characteristics that cannot be directly investigated from the study of small laboratory fish models. As a consequence, large-scale genomic research studies are increasingly being applied to farmed species of economic relevance, such as farmed rainbow trout, Atlantic salmon, tilapia, catfish, and sea bass. Accordingly, we describe the utilization of molecular cloning and gene expression analysis in cultured European sea bass (Dicentrarchus labrax) to generate ''transcriptomefocused'' information which may enable a better understanding of the transcriptional programs that underlie fish respiratory physiology and immune response pathways.

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Harmful algal bloom toxins alter c-Fos protein expression in the brain of killifish, Fundulus heteroclitus

Cited by 36 publications

References 32 publications

What is environmental stress? Insights from fish living in a variable environment

What is environmental stress? Insights from fish living in a variable environment

Chemical Defenses: From Compounds to Communities

Molecular cloning and gene expression analysis in aquaculture science: a review focusing on respiration and immune responses in European sea bass (Dicentrarchus labrax)

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