Hard X-ray Phase-Contrast Tomographic Nanoimaging

Stampanoni, Marco; Marone, Federica; Vila‐Comamala, Joan; Gorelick, Sergey; Dávid, Christian; Trtik, Pavel; Jefimovs, Konstantins; McNulty, Ian; Eyberger, Catherine; Lai, Barry

doi:10.1063/1.3625348

Cited by 6 publications

(6 citation statements)

References 11 publications

(13 reference statements)

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“…A series of synchrotron radiation-based phase contrast X-ray tomographic nanoscopy (srPCXTN) experiments [23] were conducted to document the swelling/shrinkage behaviour of micropillars at the different environmental conditions. The tomographic data were acquired at the TOMCAT beamline of the Swiss Light Source, Paul Scherrer Institut (PSI, Villigen, Switzerland).…”

Section: Phase Contrast X-ray Nano-tomographymentioning

confidence: 99%

“…In this work, we quantify the three-dimensional sorptioninduced swelling and shrinkage of the S2 layer of latewood cell walls by high-resolution synchrotron radiation-based phase contrast X-ray nano-tomography (srPCXTN) which provides images with spatial resolution in the sub-100 nm range [23]. With the aid of the FIB technique, micropillars with a cross section of the size of a few micrometres are fabricated from the S2 layer of the latewood cell walls of Norway spruce softwood.…”

Section: Introductionmentioning

confidence: 99%

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Hygroscopic swelling and shrinkage of latewood cell wall micropillars reveal ultrastructural anisotropy

Rafsanjani

Stiefel

Jefimovs

et al. 2014

J. R. Soc. Interface.

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We document the hygroscopic swelling and shrinkage of the central and the thickest secondary cell wall layer of wood (named S2) in response to changes in environmental humidity using synchrotron radiation-based phase contrast X-ray tomographic nanoscopy. The S2 layer is a natural fibre-reinforced nanocomposite polymer and is strongly reactive to water. Using focused ion beam, micropillars with a cross section of few micrometres are fabricated from the S2 layer of the latewood cell walls of Norway spruce softwood. The thin neighbouring cell wall layers are removed to prevent hindering or restraining of moisture-induced deformation during swelling or shrinkage. The proposed experiment intended to get further insights into the microscopic origin of the anisotropic hygro-expansion of wood. It is found that the swelling/shrinkage strains are highly anisotropic in the transverse plane of the cell wall, larger in the normal than in the direction parallel to the cell wall's thickness. This ultrastructural anisotropy may be due to the concentric lamellation of the cellulose microfibrils as the role of the cellulose microfibril angle in the transverse swelling anisotropy is negligible. The volumetric swelling of the cell wall material is found to be substantially larger than the one of wood tissues within the growth ring and wood samples made of several growth rings. The hierarchical configuration in wood optimally increases its dimensional stability in response to a humid environment with higher scales of complexity.

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Section: Phase Contrast X-ray Nano-tomographymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hygroscopic swelling and shrinkage of latewood cell wall micropillars reveal ultrastructural anisotropy

Rafsanjani

Stiefel

Jefimovs

et al. 2014

J. R. Soc. Interface.

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“…(step 2) A zoom into the selected ROI was made by removing the PCO2000 detector and using the nanoscopic configuration with 84 nm pixel size, as described in the next paragraph. Zernike phase-contrast hard X-ray nanotomography in the full-field mode [18] was applied to image the bordered pits in the cell wall of spruce wood. As shown in Figure 3, the key optical elements are a condenser, to provide a homogeneous and intense illumination at the sample position (focal point) and an objective lens, to magnify the image of the sample on the detector.…”

Section: Imaging Setupsmentioning

confidence: 99%

Micro- and Nano-Scales Three-Dimensional Characterisation of Softwood

Patera

Bonnin

2021

J. Imaging

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Understanding the mechanical response of cellular biological materials to environmental stimuli is of fundamental importance from an engineering perspective in composites. To provide a deep understanding of their behaviour, an exhaustive analytical and experimental protocol is required. Attention is focused on softwood but the approach can be applied to a range of cellular materials. This work presents a new non-invasive multi-scale approach for the investigation of the hygro-mechanical behaviour of softwood. At the TOMCAT beamline of the Paul Scherrer Institute, in Switzerland, the swelling behaviour of softwood was probed at the cellular and sub-cellular scales by means of 3D high-resolution phase-contrast X-ray imaging. At the cellular scale, new findings in the anisotropic and reversible swelling behaviour of softwood and in the origin of swelling hysteresis of porous materials are explained from a mechanical perspective. However, the mechanical and moisture properties of wood highly depend on sub-cellular features of the wood cell wall, such as bordered pits, yielding local deformations during a full hygroscopic loading protocol.

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“…Access to a ±90° range is achieved by preparing samples on cylindrical containers rather than on flat silicon-nitrides membranes like here. We implemented such a solution and we were able to perform a tomographic scan of an unstained, not cryo-cooled cell at a measured resolution of 144 nm 3 [9].…”

Section: Advanced Zernike Phase Contrast Imagingmentioning

confidence: 99%

Tomographic Hard X-ray Phase Contrast Micro- and Nano-imaging at TOMCAT

Stampanoni

Marone

Modregger

et al. 2010

AIP Conference Proceedings

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Abstract. This article illustrates the phase contrast instrumentation installed at the Tomographic Microscopy and Coherent Radiology beamline (TOMCAT) of the Swiss Light Source. Our experimental framework has been designed to extract phase information at spatial resolutions covering three orders of magnitude. For moderate (5-10 microns) resolutions we implemented a two-gratings interferometer, operated at energies between 14 and 40 keV. For high resolution (1-5 microns) we obtain phase information thanks to a modified transport of intensity approach. For very high-resolutions (0.1-0.5 microns) we developed a broadband hard X-ray full-field microscope operated in Zernikephase contrast.

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Hard X-ray Phase-Contrast Tomographic Nanoimaging

Cited by 6 publications

References 11 publications

Hygroscopic swelling and shrinkage of latewood cell wall micropillars reveal ultrastructural anisotropy

Hygroscopic swelling and shrinkage of latewood cell wall micropillars reveal ultrastructural anisotropy

Micro- and Nano-Scales Three-Dimensional Characterisation of Softwood

Tomographic Hard X-ray Phase Contrast Micro- and Nano-imaging at TOMCAT

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