Hapten-specific T cell responses to 4-hydroxy-3-nitrophenyl acetyl. XI. Pseudogenetic restrictions of hybridoma suppressor factors

Okuda, Kenji; Minami, Manabu; Dh, Sherr; Dorf, M E

doi:10.1084/jem.154.2.468

Cited by 57 publications

(62 citation statements)

References 19 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In several independent systems, I-J (or I-C) restrictions have been observed on the interactions of suppressor T cells (13)(14)(15)(16)(17)(18). In most systems, the Ts cells are stimulated by antigen priming.…”

Section: Discussionmentioning

confidence: 99%

Mechanism responsible for the induction of I-J restriction on TS3 suppressor cells.

Minami¹,

Honji²,

Dorf³

1982

The Journal of Experimental Medicine

Self Cite

View full text Add to dashboard Cite

The mechanisms responsible for the induction of I-J restrictions on third-order suppressor T cells (TS3) were analyzed. The I-J phenotype of the antigen-coupled cells used for priming restricted the specificity of the TS3 population. Thus, TS3 cells were only generated after priming with antigen-coupled I-J homologous cells. Identity at the I-JM (and I-E) subregions was sufficient for TS3 induction. Furthermore, priming of H-2 heterozygous mice with antigen-coupled parental cells generated TS3 that were restricted to the parental haplotype used for priming. The splenic cell population responsible for antigen presentation and induction of TS3 cells was fractionated. The cells involved in antigen presentation were found in the splenic adherent population and were absent in the fraction containing splenic nonadherent T and B cells. The subsequent activation and interaction of TS3 cells is also restricted by genes in the H-2 complex. The results are discussed in terms of a general mechanism responsible for the induction of restrictions in T helper and TS3 cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Mechanism responsible for the induction of I-J restriction on TS3 suppressor cells.

Minami¹,

Honji²,

Dorf³

1982

The Journal of Experimental Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

“…There are descriptions of suppressor cells acting at the afferent and efferent stages of contact and delayed hypersensitivity response; haptens [ [18,19] have described the Tsl-Ts3 system. They believe that the Tsl induces the Ts2, then activates the Ts3 (which arises by an independent mode of immunization).…”

Section: Discussionmentioning

confidence: 99%

Immunosuppression in experimental cryptococcosis in rats. Induction of afferent T suppressor cells to a non-related antigen

et al. 1987

View full text Add to dashboard Cite

To demonstrate the nature of the suppressor cells elicited in rats infected with Cryptococcus neoformans and immunized with human serum albumin (HSA), spleen mononuclear (SpM) cells were fractionated through a nylon wool column. The adherent and non-adherent populations were collected and transferred to syngeneic rats. In all cases, the non-adherent or T-enriched cells adoptively transferred suppression to HSA, however, the suppressive effects of the non-adherent cells were never as great as those of the unpassed population of SpM cells. The fractions adherent to nylon wool also diminished the delayed-type hypersensitivity response to HSA although this was not significant, but glass-adherent cells did exhibit significant suppressor activity. Immunized, non-infected rats were used as donor controls. Furthermore, we showed that the T-enriched-cells are sensitive to treatment with low doses of cyclophosphamide and that they bind HSA. These data indicate that immune suppression of the induction of the delayed-type hypersensitivity response to HSA in cryptococcosis can occur as a result of infection with C. neoformans, and that at least one mechanism involved is the induction of adherent and non-adherent suppressor cells. Characterization of the nonadherent cells indicates that they are Tsl cells.

show abstract

“…Such cells could serve to juxtapose the TsF2 with the Ts3 cells, thereby permitting the interactions required for Ts~ activation. Furthermore, since suppressor cell interactions can occur in the absence of an external source of antigen (13,25), the Igh restriction may reflect an antiidiotypic internal image (which substitutes for antigen). This hypothesis further suggests that such a system of internal images is in some fashion controlled by genes linked to the Igh complex.…”

Section: Discussionmentioning

confidence: 99%

“…The method of absorption and elution of TsF using protein-conjugated Sepharose-4B columns were described in detail previously (25).…”

Section: Preparation Of Ts3mentioning

confidence: 99%

The role of I-J and Igh determinants on F1-derived suppressor factor in controlling restriction specificity.

Minami

Aoki

Honji

et al. 1983

The Journal of Experimental Medicine

Self Cite

View full text Add to dashboard Cite

In the 4-hydroxy-3-nitrophenyl acetyl (NP) contact sensitivity system, the activity of third-order suppressor cells and their factors is restricted by H-2(I-J) and Igh linked genes. The present report analyzes the specificity of NP-specific Ts3 cells and factors derived from H-2 and Igh heterozygous (B6 X C3H)F1 mice. Two approaches were used. First, heterogeneous populations of F1 Ts3 cells were activated in vitro and then assayed in Ts3-depleted recipients which carried different combinations of H-2 and Igh alleles. The second approach was to hybridize the Ts3 cells and analyze the specificity of the F1-derived TsF3. The combined data demonstrated four functionally distinct populations of Ts3 cells. The activity of each population was restricted by a particular combination of H-2 and Igh haplotypes. Thus, Ts3 cells derived from F1 donors can demonstrate an apparent scrambling of H-2 and Igh restriction specificities. There was functional allelic exclusion of the H-2(I-J) and Igh determinants expressed on (B6 X C3H)F1 hybridoma-derived TsF3. Thus, TsF3 from each cloned hybridoma line expressed only one set of I-J and Igh determinants. Furthermore, there was a complete correlation between the I-J and Igh linked determinants expressed on TsF3 and the restriction specificity. In view of the recent findings on the molecular biology of the I-J region, an alternative interpretation of the role of I-J determinants on suppressor cells and factors is offered.

show abstract

Hapten-specific T cell responses to 4-hydroxy-3-nitrophenyl acetyl. XI. Pseudogenetic restrictions of hybridoma suppressor factors

Cited by 57 publications

References 19 publications

Mechanism responsible for the induction of I-J restriction on TS3 suppressor cells.

Mechanism responsible for the induction of I-J restriction on TS3 suppressor cells.

Immunosuppression in experimental cryptococcosis in rats. Induction of afferent T suppressor cells to a non-related antigen

The role of I-J and Igh determinants on F1-derived suppressor factor in controlling restriction specificity.

Contact Info

Product

Resources

About