Hapten reactive inducer T cells. II. Evidence that a secreted form of the T cell receptor induces antibody production.

Clayberger, Carol; Cantor, Harvey

doi:10.1084/jem.158.6.1881

Cited by 8 publications

(1 citation statement)

References 24 publications

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“…The inactivating molecule is not the antigen-binding T cell receptor secreted by Ly-1 T cell clones. On the contrary, whereas antigenbinding material secreted by a TNP-specific Ly-1 T cell clone provides positive signals for B cell maturation and IgM secretion (47,48), the same T cell clone inhibits the secretion of IgG when it makes an antigen-specific and I regionrestricted contact with B cells (data not shown). If receptor blockade is the mechanism of inactivation by the clones, then the molecules causing the blockade are bound so tightly to the Ig receptor that they can not be removed by extensive washing (Table III).…”

Section: Characteristics Of Inhibition Effective Inhibition At Low Amentioning

confidence: 99%

A subset of Ly-1 inducer T cell clones activates B cell proliferation but directly inhibits subsequent IgG secretion.

Friedman¹,

Sillcocks²,

Rao³

et al. 1985

The Journal of Experimental Medicine

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Ly-1 inducer T cells play a critical role in the regulation of antibody synthesis. One subset of Ly-1 T inducer cells (Qa-1-) can activate B lymphocytes to secrete IgM (1). A second (Qa-1 +) stimulates Ly-l,2 + T cells to differentiate into Ly-2 + T suppressor cells (1-3) that inactivate both subsets of inducer T cells, resulting in reduced levels of antibody formation and suppression.Recent reports (4-7) have indicated that T cells can also inhibit antibody responses without induction of Ly-2 T suppressors. Heterogeneous or cloned Ly-1 T cells failed to function as T helper (Th) 1 cells and inhibited primary IgM responses. This reflected direct inhibition of B cell maturation into IgM-secreting cells by the Ly-l:Qa-1 + fraction of inducer cells (4, 5). The nonhelper Ly-1 T cell induced B cell proliferation but inhibited primary IgM response to phosphorylcholine-ovalbumin (PC-OVA): suppression was I-A-restricted and required a hapten-carrier bridge between the B cell and the Ly-1 T cell clone (5). Inhibition of secondary responses with some nonhelper Ly-1 ÷ T cells (6, 7) has also been observed. In this case, T cell clones prevent an antigen-specific, major histocompatibility complex (MHC)-restricted interaction between carrier-specific Th cells and B cells.In the present study, we further analyzed the mechanism of inhibition of B cell responses in vitro, using Ly-1 T cell clones. We found that two Ly-1 T cell clones did not induce B cells to secrete antigen-specific IgG. Instead, these clones inhibited secretion of IgG antibody to T-dependent soluble antigens. Inactivation of the B cell occurred early and required cell-cell contact that is both I region restricted and antigen specific, resulting in an early proliferative response by antigen-specific memory B cells, without subsequent differentiation into antibody-secreting cells.

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Section: Characteristics Of Inhibition Effective Inhibition At Low Amentioning

confidence: 99%

A subset of Ly-1 inducer T cell clones activates B cell proliferation but directly inhibits subsequent IgG secretion.

Friedman¹,

Sillcocks²,

Rao³

et al. 1985

The Journal of Experimental Medicine

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HLA class II restriction governing cell cooperation between antigen‐specific helper T lymphocytes, B lymphocytes and monocytes for in vitro antibody production to influenza virus

Fischer

Sterkers

Charron³

et al. 1985

Eur J Immunol

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To study HLA class II compatibility requirement for in vitro antibody production to influenza virus, semipurified T lymphocytes, B lymphocytes and monocytes from HLA-typed responder donors were used. The presence of the three subpopulations was required for antibody production while a mixture of only two of those was ineffective. When using fresh T lymphocytes which exert an allogeneic suppressive effect and may also exhibit allogeneic helper activity, it was not possible to conclude an HLA class II-linked restriction of T-B cell cooperation although there was a suggestion of it. However, a grown H3 hemagglutinin-specific T cell line (L2), previously shown to be restricted by HLA-DR molecule (DR1) for interaction with antigen-presenting cells and devoid of allogeneic reactivity, exerts an HLA class II-restricted helper activity. This was demonstrated by various combinations of HLA-DR semi-compatible or incompatible B lymphocytes and/or monocytes with L2 T cells. The restriction element was identified as an HLA-DR determined since HLA-DC-compatible, HLA-DR-incompatible B lymphocytes were not helped by L2 T cells. In addition, monoclonal anti-HLA-DR but not anti-HLA-DC antibodies directed to the relevant specificity did inhibit the antigen-specific helper activity. We present evidence that not only T monocyte but also T-B and/or T-B-monocyte interactions are HLA class II restricted.

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Antigen-Specific T Cell Helper Factors

Woody

Lamb

Zanders

et al. 1985

Human T Cell Clones

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Hapten reactive inducer T cells. II. Evidence that a secreted form of the T cell receptor induces antibody production.

Cited by 8 publications

References 24 publications

A subset of Ly-1 inducer T cell clones activates B cell proliferation but directly inhibits subsequent IgG secretion.

A subset of Ly-1 inducer T cell clones activates B cell proliferation but directly inhibits subsequent IgG secretion.

HLA class II restriction governing cell cooperation between antigen‐specific helper T lymphocytes, B lymphocytes and monocytes for in vitro antibody production to influenza virus

Antigen-Specific T Cell Helper Factors

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