Hapten-protein conjugates prepared by the mixed anhydride method

Development of a sensitive competitive indirect ELISA method for determination of ochratoxin A levels in cereals originating from Nanjing, China

“…The scheme of different conjugate protein and synthesis methods for the immunization antigen (OTAeBSA) and the coating antigen (OTAeKLH) was beneficial for screening for specific antibody (Clarke et al, 1993). The CR of the antibody to OTA analogs or to conjugate protein, which partly came from the by-product in complete antigen synthesis (Gendloff et al, 1986), might be reduced in this way, for example, the CR of anti-OTA antibody to OTa and BSA, but unfortunately, it was not tested in this study.…”

Selection, characterization and application of aptamers targeted to Aflatoxin B2

“…Activation of AFB2 was conducted as described by Morgan (Gendloff et al, 1986) with some modifications. Four hundred microliters of AFB2 (2.5 mg/mL, dissolved in N,N-dimethylformamide), 2 mg of carboxymethoxylamine hemihydrochloride and 12 mL of a mixed solution (2 mL of water, 2 mL of pyridine and 8 mL of methanol) were added to a round-bottom flask for oil bath refluxing at 85 C (3 h).…”

Technical note: Development of an enzyme-linked immunosorbent assay for the determination of florfenicol and thiamphenicol in swine feed1

“…The coating antigens, FF-SH-OVA and FF-MH-OVA, were synthesized by mixed anhydride reaction (Gendloff et al, 1986). These contents were dialyzed against NaCl (0.9%) for 72 h at 4°C and then centrifuged at 1,000 x 5 for 5 min at 4°C to discard sediment.…”