Hapten Design and Development of Polarization Fluoroimmunoassay for Nonylphenol

Yakovleva, Julia; Žeravík, Jiří; Michura, Irina V.; Formanovsky, A. A.; Fránek, Milan; Eremin, Sergei A.

doi:10.1080/0306731021000060029

Cited by 19 publications

(23 citation statements)

References 25 publications

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“…Protein conjugate 4AP-BSA was synthesized by cross-linking via GA according to Ref. [19]. Brief descriptions of the syntheses are given below.…”

Section: Synthesis Of Haptens and Protein Conjugatesmentioning

confidence: 99%

“…Another property of AP which hampers the production of high-affinity antibodies is their structural heterogeneity because of the large number of isomers with different alkyl chain-lengths and positions on the benzene ring. Although polyclonal [16,19] and monoclonal [20] antibodies against NP have been described, it has not been possible to develop a sensitive and, at the same time, specific assay for heterogeneous NP mixtures.…”

Section: Introductionmentioning

confidence: 98%

“…FPIA is a rapid homogeneous immunoassay technique, especially suitable for the screening detection of target analytes, because the assay time per sample is about 1 min. In our previous work hapten design was based either on an NP derivative with an active terminal carboxylic group, ω-(4-hydroxyphenyl)nonanoic acid [16], or on 4-aminophenol cross-linked to the carrier protein via glutaraldehyde [19]. In this investigation we describe antibodies raised against new derivatives: …”

Section: Introductionmentioning

confidence: 98%

“…We recently reported the development of a fluorescence polarization immunoassay (FPIA) for screening detection of NP [19]. FPIA is a rapid homogeneous immunoassay technique, especially suitable for the screening detection of target analytes, because the assay time per sample is about 1 min.…”

Section: Introductionmentioning

confidence: 99%

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Express detection of nonylphenol in water samples by fluorescence polarization immunoassay

et al. 2003

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The development of express method for detection of endocrine-disrupting chemicals (EDC) such as alkylphenols is required for ecological monitoring. Several attempts have been made to produce antibodies against 4-nonylphenol (NP) in recent years. This work describes the production of new antibodies against NP and also summarizes the characterization of antibodies obtained earlier. Three approaches used to produce alkylphenol-specific antibodies are compared; these are based on: 1. omega-(4-hydroxyphenyl)nonanoic or omega-(4-hydroxyphenyl)heptanoic acid NP derivatives designed to mimic the linear NP isomer; 2. 4-aminophenol, which potentially mimics various substituted phenolic compounds with different side-chain structures at position 4 of the benzene ring; and 3. a mixture of branched NP isomers, conjugated to the carrier protein via a benzene ring by the Mannich reaction, and expected to be the closest mimic of NP structure by preserving its natural alkyl moiety.Fluorescence polarization immunoassays based on different combinations of antibody and labeled antigen for screening detection of NP were developed and structural aspects of assay sensitivity and specificity were investigated. The assays based on the antisera raised against omega-(4-hydroxyphenyl)nonanoic acid and NP conjugate via Mannich reaction are capable of express detection of NP with detection limit of 7 microg mL(-1 )and assay dynamic range of 18-300 microg mL(-1).

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“…Protein conjugate 4AP-BSA was synthesized by cross-linking via GA according to Ref. [19]. Brief descriptions of the syntheses are given below.…”

Section: Synthesis Of Haptens and Protein Conjugatesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Express detection of nonylphenol in water samples by fluorescence polarization immunoassay

et al. 2003

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“…No cross-reactivity interference was indicated for linear alkylbenzensulfonates and phenolic compounds. These antibodies were employed for development of ELISA and dipstick tests to measure nonylphenol in water samples (Samsonova et al, 2003) and for development of polarisation fluoroimmunoassay (Yakovleva et al, 2003). A rapid Biacore biosensor immunoassay of 4-nonylphenol was optimised and applied for shellfish analysis (Samsonova et al, 2004).…”

Section: Surfactants and Nonylphenolmentioning

confidence: 99%

Antibody based methods for environmental and food analysis: a review

Fránek¹,

Hruška²

2005

Vet. Med. - Czech

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Antibodies have widely been used as analytical tools in various assays and techniques developed for clinical chemistry and endocrinology and for food and environmental research and risk control. Antibody development in the Veterinary Research Institute, Brno, and their application in ELISA and related techniques such as immunosensors has been directed especially to phenoxyacetic acid herbicides, s-triazine herbicides, sulfonylurea herbicides, polychlorinated biphenyls, surfactants (linear alkylbenzene sulphonates) and toxic metabolites (nonylphenol), and selected veterinary drugs (namely nitrofurans and sulfonamides). This paper provides an overview of progress achieved in the production of key immunoreagents in this laboratory (and in some cooperating laboratories)during the last 15 years. A comprehensive analysis of papers published on immunoassays and biosensors used in food and environmental research since 1980 demonstrates a rapid increase of publications on “ELISA and immunoassays” since 1991 (more than 500 papers were published each year since 1996). More than 200 papers on “biosensors” have been published each year since 2001. Atrazine was the most frequently found key word with ELISA and immunoassays: 438 papers were written by 971 authors from 308 institutions. The Web of Science® database is a useful tool for an assessment of the researcher’s and institution’s interest in the specific topics of research.

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Fluorescence polarization immunoassays and related methods for simple, high-throughput screening of small molecules

2008

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Fluorescence polarization immunoassay (FPIA) is a homogeneous (without separation) competitive immunoassay method based on the increase in fluorescence polarization (FP) of fluorescent-labeled small antigens when bound by specific antibody. The minimum detectable quantity of FPIAs with fluorescein label (about 0.1 ng analyte) is comparable with chromatography and ELISA methods, although this may be limited by sample matrix interference. Because of its simplicity and speed, FPIA is readily automated and therefore suitable for high-throughput screening (HTS) in a variety of application areas. Systems that involve binding of ligands to receptor proteins are also susceptible to analysis by analogous FP methods employing fluorescent-labeled ligand and HTS applications have been developed, notably for use in candidate drug screening.

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Hapten Design and Development of Polarization Fluoroimmunoassay for Nonylphenol

Cited by 19 publications

References 25 publications

Express detection of nonylphenol in water samples by fluorescence polarization immunoassay

Express detection of nonylphenol in water samples by fluorescence polarization immunoassay

Antibody based methods for environmental and food analysis: a review

Fluorescence polarization immunoassays and related methods for simple, high-throughput screening of small molecules

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