Haplotypes within the regulatory region of MYL4 are associated with pig muscle fiber size

Dong, Shixiong; Han, Yaling; Zhang, Jian; Ye, Yourong; Duan, Mengqi; Wang, Kejun; Wei, Mingbang; Chamba, Yangzom; Shang, Peng

doi:10.1016/j.gene.2022.146934

Cited by 3 publications

(4 citation statements)

References 41 publications

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“…By utilizing the STRING database to construct a protein-protein interaction (PPI) network, the MYL4 gene was identified as a key candidate gene that could influence pig muscle growth. This finding aligns with the results of research conducted by Dong S [12] and colleagues. Using bioinformatics software to predict and analyze the structural characteristics of the MYL4 gene protein, it was found that the porcine MYL4 protein is primarily composed of α-helices and random coils.…”

Section: Discussionsupporting

confidence: 92%

“…The protein produced by MYL4 is an essential component of the myofilament complex [11]. Its expression product possesses Ca 2+ binding and motor activity, participating in the structural components of skeletal muscle, and is associated with muscle development and sarcomere contraction [12]. The most current research on the MYL4 gene focuses on cardiomyopathy [13,14].…”

Section: Introductionmentioning

confidence: 99%

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The Role of the MYL4 Gene in Porcine Muscle Development and Its Molecular Regulatory Mechanisms

Ye,

Wu,

Wang

et al. 2024

Animals

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Muscle growth stands as a pivotal economic trait within pig production, governed by a complex interplay of multiple genes, each playing a role in its quantitative manifestation. Understanding the intricate regulatory mechanisms of porcine muscle development is crucial for enhancing both pork yield and quality. This study used the GSE99749 dataset downloaded from the GEO database, conducting a detailed analysis of the RNA-seq results from the longissimus dorsi muscle (LD) of Tibetan pigs (TP), Wujin pigs (WJ) and large white pigs (LW) at 60 days of gestation, representing diverse body sizes and growth rates. Comparative analyses between TPvsWJ and TPvsLW, along with differential gene expression (DEG) analysis, functional enrichment analysis, and protein–protein interaction (PPI) network analysis, revealed 1048 and 1157 significantly differentially expressed genes (p < 0.001) in TPvsWJ and TPvsLW, respectively. With stricter screening criteria, 37 DEGs were found to overlap between the 2 groups. PPI analysis identified MYL5, MYL4, and ACTC1 as the three core genes. This article focuses on exploring the MYL4 gene. Molecular-level experimental validation, through overexpression and interference of the MYL4 gene combined with EDU staining experiments, demonstrated that overexpression of MYL4 significantly promoted the proliferation of porcine skeletal muscle satellite cells (PSMSC), while interference with MYL4 inhibited their proliferation. Furthermore, by examining the effects of overexpressing and interfering with the MYL4 gene on the muscle hypertrophy marker Fst gene and the muscle degradation marker FOXO3 gene, the pivotal role of the MYL4 gene in promoting muscle growth and preventing muscle degradation was further confirmed. These findings offer a new perspective on the molecular mechanisms behind porcine muscle growth and development, furnishing valuable data and insights for muscle biology research.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

The Role of the MYL4 Gene in Porcine Muscle Development and Its Molecular Regulatory Mechanisms

Ye,

Wu,

Wang

et al. 2024

Animals

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“…The identification of numerous candidate genes associated with economic traits in this study contributes to our understanding of the genetic factors influencing these traits in pigs ( Table 1 ). Among the identified genes, MYL4 was found to exhibit differential expression in the longest muscle tissue of the pig’s back, which correlated with variations in the number of muscle fibres within the tissue [ 16 ]. Other genes of interest include FGFR2 , which has been recognized as a crucial regulator of myogenesis during skeletal muscle development and regeneration [ 17 ], and SFRP1 , predicted to be targeted by miRNA-1/206, and implicated in muscle cell proliferation and prenatal skeletal muscle development [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

Genomic Scan for Runs of Homozygosity and Selective Signature Analysis to Identify Candidate Genes in Large White Pigs

Yin,

Wang,

Zhou

et al. 2023

IJMS

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Large White pigs are extensively utilized in China for their remarkable characteristics of rapid growth and the high proportion of lean meat. The economic traits of pigs, comprising reproductive and meat quality traits, play a vital role in swine production. In this study, 2295 individuals, representing three different genetic backgrounds Large White pig populations were used: 500 from the Canadian line, 295 from the Danish line, and 1500 from the American line. The GeneSeek 50K GGP porcine HD array was employed to genotype the three pig populations. Firstly, genomic selective signature regions were identified using the pairwise fixation index (FST) and locus-specific branch length (LSBL). By applying a top 1% threshold for both parameters, a total of 888 candidate selective windows were identified, harbouring 1571 genes. Secondly, the investigation of regions of homozygosity (ROH) was performed utilizing the PLINK software. In total, 25 genomic regions exhibiting a high frequency of ROHs were detected, leading to the identification of 1216 genes. Finally, the identified potential functional genes from candidate genomic regions were annotated, and several important candidate genes associated with reproductive traits (ADCYAP1, U2, U6, CETN1, Thoc1, Usp14, GREB1L, FGF12) and meat quality traits (MiR-133, PLEKHO1, LPIN2, SHANK2, FLVCR1, MYL4, SFRP1, miR-486, MYH3, STYX) were identified. The findings of this study provide valuable insights into the genetic basis of economic traits in Large White pigs and may have potential use in future pig breeding programs.

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“…Ghazizadeh et al found increased retinoic acid synthesis and actin disorder in MYL4 mutant cell lines and zebrafish mutation models, indicating that MYL4 interacts with cytoskeletal actin both in vitro and in vivo [ 17 ]. Dong et al identified nine SNPs in Pig’s 5′ flanking region of MYL4 genes; these nine SNPs increased the mRNA and protein expression of MYL4 in porcine muscles and leads to an increase in the number of muscle fibers in porcine Longissimus dorsi muscle [ 18 ]. Given that MYL4 is involved in the whole process of skeletal muscle, the effect of MYL4 on cells within muscle tissue requires further exploration.…”

Section: Introductionmentioning

confidence: 99%

Molecular Mechanism of MYL4 Regulation of Skeletal Muscle Development in Pigs

et al. 2023

Genes

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The processes of muscle growth and development, including myoblast proliferation, migration, differentiation, and fusion, are modified by a variety of regulatory factors. MYL4 plays an important role in atrial development, atrial cardiomyopathy, muscle-fiber size, and muscle development. The structural variation (SV) of MYL4 was found via the de novo sequencing of Ningxiang pigs, and the existence of SV was verified in the experiments. The genotype distribution of Ningxiang pigs and Large White pigs was detected, and it was found that Ningxiang pigs were mainly of the BB genotype and that Large White pigs were mainly of the AB genotype. However, the molecular mechanisms behind the MYL4-mediated regulation of skeletal muscle development need to be deeply explored. Therefore, RT-qPCR, 3′RACE, CCK8, EdU, Western blot, immunofluorescence, flow cytometry, and bioinformation analysis were used to explore the function of MYL4 in myoblast development. The cDNA of MYL4 was successfully cloned from Ningxiang pigs, and its physicochemical properties were predicted. The expression profiles in six tissues and four stages of Ningxiang pigs and Large White pigs were found to be the highest in the lungs and 30 days after birth. The expression of MYL4 increased gradually with the extension of the myogenic differentiation time. The myoblast function test showed that the overexpression of MYL4 inhibited proliferation and promoted apoptosis and differentiation. The knockdown of MYL4 showed the opposite result. These results enhance our understanding of the molecular mechanisms of muscle development and provide a solid theoretical foundation for further exploring the role of the MYL4 gene in muscle development.

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Haplotypes within the regulatory region of MYL4 are associated with pig muscle fiber size

Cited by 3 publications

References 41 publications

The Role of the MYL4 Gene in Porcine Muscle Development and Its Molecular Regulatory Mechanisms

The Role of the MYL4 Gene in Porcine Muscle Development and Its Molecular Regulatory Mechanisms

Genomic Scan for Runs of Homozygosity and Selective Signature Analysis to Identify Candidate Genes in Large White Pigs

Molecular Mechanism of MYL4 Regulation of Skeletal Muscle Development in Pigs

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