Haplotype Mapping and Sequence Analysis of the Mouse Nramp Gene Predict Susceptibility to Infection with Intracellular Parasites

Incremental expression of Tlr4 correlates with mouse resistance to Salmonella infection and fine regulation of relevant immune genes

“…The original B10/ScNCr strain has no Tlr4 because of a 75 kb chromosomal deletion encompassing the Tlr4 locus 40 and carries a mutated, nonfunctional allele at Nramp1 (Nramp1 Asp169 )). 41 Because of the known major effect of Nramp1 on mouse salmonellosis, we generated a congenic strain, B10/ScNCr. C3H-Nramp1 (or B10.C3H-Nramp1), through 10 consecutives backcross generations to be used in a cross with our transgenic strain.…”

Genetic regulation of host responses to Salmonella infection in mice

“…55 Predicted protein sequence analysis of Nramp1 between Ity r and Ity s strains revealed a single mutation resulting in glycine to aspartic acid substitution at position 169. 56 This mutation results in a complete lack of function of Nramp1 in susceptible mice. 57 The identity of Nramp1, Bcg, Ity and Lsh was confirmed by targeted disruption of Nramp1 in mice and phenotypic comparison among different genotypes of mice during experimental infections with Salmonella Typhimurium, M. bovis and L. donovani.…”

Identification of genetic loci controlling bacterial clearance in experimental Salmonella enteritidis infection: an unexpected role of Nramp1 (Slc11a1) in the persistence of infection in mice

“…Ses2 explained 6% of the genetic variance and Ses3, 4% (Table 2). In these cases also, the C57BL/6J allele is protective and contributes in a recess- 33,9,36,25 D7Mit76,83,62,31,323,38,237,44,189 D8Mit3,41,56 D9Mit218,25,306,37 D10Mit80,38,42,10,205 D11Mit77,20,5,4,124,61 D12Mit182,49,12,59,46,60,14,Nds2 D13Mit236,139,147,30,36,151 D14Mit11,52,30 D15Mit12,26,46,47,29,42,242,159,35 D16Mit81,4,47,…”

“…The physical order of the markers located around the marker with the peak LOD score (D1Mcg5*) is D1Mcg2, D1Mcg3, D1Mcg5, D1Mcg7, D1Mit46 and D1Mit134. 60 ive model of transmission. We tested for the presence of interaction among Ses1-Ses3 loci by examining all pairwise combinations.…”