Haplotype diversity in medically important red scorpion (Scorpiones: Buthidae: Hottentotta tamulus) from India

Suranse, Vivek; Sawant, Nitin; Bastawade, D. B.; Dahanukar, Neelesh

doi:10.1007/s12041-019-1058-5

Cited by 3 publications

(4 citation statements)

References 32 publications

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“…1). These results are in agreement with values reported by Yamashita and Rhoads (2013) in Cen-truroides vittatus populations from the United States (Hd = 0.86 to 1.00) and Suranse et al (2019) in H. tamulus species (Hd = 0.94) using the same gene.…”

Section: Discussionsupporting

confidence: 92%

“…This pattern coincides with similar studies of genetic structuring obtained in populations of Centruroides vittatus in the United States with values of Fst from 0.86 to 1.00 (Yamashita and Rhoads, 2013). Mountainous systems, similar to the Moanicum sector play an important role in shaping the current distribution of various types of genetic data, such as the genetic structure of scorpions (Suranse et al, 2017) and phenetic data like venom peptide composition (Suranse et al, 2019).…”

Section: Discussionsupporting

confidence: 88%

“…This investigation was aimed to analyze both genetic variation and peptide masses differentiation of R. junceus venom in six districts of Moanicum sector of Cuba. For the genetic characterization, the gene encoding cytochrome oxidase subunit I was chosen in agreement with its frequently use in scorpion populations molecular studies (Ojanguren-Affiltro et al, 2016;Suranse et al, 2019). To the best of our knowledge, there is no evidence about the genetic differentiation and the intraspecific variation in the venom peptides of R. junceus in Cuba using these molecular approaches.…”

Section: Introductionmentioning

confidence: 99%

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Variation of cytochrome oxidase-I gene and venom proteins of Rhopalurus junceus populations in the Moanicum sector: Pharmacological implications

Rodríguez-Ravelo¹,

Ruíz-Urquiola²,

Possani³

et al. 2021

J Pharm Pharmacogn Res

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Context: In Cuba, the venom of the endemic scorpion Rhopalurus junceus has been used in traditional medicine for cancer treatment. The genetic variability in populations from distinct origin and differences in the composition of venom –a little explored topic- could influence the pharmacological effects of products used in medical practice. Aims: To characterize the populations of R. junceus from six different districts of the Moanicum sector in northeastern Cuba, taking into account the variations in the sequences of cytochrome oxidase subunit I gene (COI) and peptide masses of venom. Methods: For the genetic characterization, a 658 bp gene fragment of COI was amplified by PCR. The genetic structure of populations was analyzed hierarchically, using an analysis of molecular variance (AMOVA). In proteomic analysis, molecular masses of venom constituents were arranged in increasing order, and different components were considered when masses differed by 2 Da. Results: R. junceus species in the six districts studied in the Moanicum sector belong to different populations. The sequences of COI gene showed high levels of genetic variability, recovering 47 haplotypes (87% unique). The composition of the venom was also significantly different between the districts. The most abundant components presented masses of 3-6 kDa (K+-channel specific peptides) and 6,1-10 kDa (Na+-channel specific peptides). Conclusions: Populations of R. junceus showed high genetic differentiation and a broad intraspecific variation in the venom peptides (3-10 kDa) in six districts of Moanicum sector of Cuba. This richness in scorpion varieties with different toxins profile should be considered for pharmacological applications.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

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Variation of cytochrome oxidase-I gene and venom proteins of Rhopalurus junceus populations in the Moanicum sector: Pharmacological implications

Rodríguez-Ravelo¹,

Ruíz-Urquiola²,

Possani³

et al. 2021

J Pharm Pharmacogn Res

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“…Geographical variation in sting severity has been reported in India (Reddy, 2013;Suranse et al, 2019) (Figure 2) and is a likely consequence of variation in population genetic structure, which drives phenotypic differences in venom composition (Newton et al, 2007). Several populations of Indian red scorpions collected from eight locations in Maharashtra (Bhate plateau, Sangameshwar, Jejuri, Shindavane, Pashan, Alandi, Kalyan, and Jalna) exhibited moderate genetic variation, with regression analysis suggesting that the genetic distance of subspecies increases by 0.006% (95%CI: 0.003-0.010%) per Kilometre of geographical separation (Suranse et al, 2017).…”

Section: Diversity Of Indian Red Scorpion Venommentioning

confidence: 99%

Biochemical and Proteomic Characterization, and Pharmacological Insights of Indian Red Scorpion Venom Toxins

2021

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The Indian red scorpion (Mesobuthus tamulus) is one of the world’s deadliest scorpions, with stings representing a life-threatening medical emergency. This species is distributed throughout the Indian sub-continent, including eastern Pakistan, eastern Nepal, and Sri Lanka. In India, Indian red scorpions are broadly distributed in western Maharashtra, Saurashtra, Kerala, Andhra Pradesh, Tamil Nadu, and Karnataka; however, fatal envenomations have been recorded primarily in the Konkan region of Maharashtra. The Indian red scorpion venom proteome comprises 110 proteins belonging to 13 venom protein families. The significant pharmacological activity is predominantly caused by the low molecular mass non-enzymatic Na+ and K+ ion channel toxins. Other minor toxins comprise 15.6% of the total venom proteome. Indian red scorpion stings induce the release of catecholamine, which leads to pathophysiological abnormalities in the victim. A strong correlation has been observed between venom proteome composition and local (swelling, redness, heat, and regional lymph node involvement) and systemic (tachycardia, mydriasis, hyperglycemia, hypertension, toxic myocarditis, cardiac failure, and pulmonary edema) manifestations. Immediate administration of antivenom is the preferred treatment for Indian red scorpion stings. However, scorpion-specific antivenoms have exhibited poor immunorecognition and neutralization of the low molecular mass toxins. The proteomic analysis also suggests that Indian red scorpion venom is a rich source of pharmacologically active molecules that may be envisaged as drug prototypes. The following review summarizes the progress made towards understanding the venom proteome of the Indian red scorpion and addresses the current understanding of the pathophysiology associated with its sting.

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Genetic diversity of medically important scorpions of the genus Centruroides (Buthidae) from Panama including two endemic species

Escudero-Sanjur

Castro‐Pérez

Patiño

et al. 2022

J Genet

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Haplotype diversity in medically important red scorpion (Scorpiones: Buthidae: Hottentotta tamulus) from India

Cited by 3 publications

References 32 publications

Variation of cytochrome oxidase-I gene and venom proteins of Rhopalurus junceus populations in the Moanicum sector: Pharmacological implications

Variation of cytochrome oxidase-I gene and venom proteins of Rhopalurus junceus populations in the Moanicum sector: Pharmacological implications

Biochemical and Proteomic Characterization, and Pharmacological Insights of Indian Red Scorpion Venom Toxins

Genetic diversity of medically important scorpions of the genus Centruroides (Buthidae) from Panama including two endemic species

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