Haploidy in important crop plants — potato

Veilleux, Richard E.

doi:10.1007/978-94-017-1858-5_3

Cited by 9 publications

(3 citation statements)

References 49 publications

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“…In order to breed at the diploid level, dihaploid potato genotypes 2n ¼ 2x ¼ 24 ð Þmay be extracted from tetraploid potatoes 2n ¼ 4x ¼ 48 ð Þby anther culture (Veilleux 1996) or pseudogamy (Van Breukelen et al 1977).…”

Section: Introductionmentioning

confidence: 99%

Oryzalin Treatment of Potato Diploids Yields Tetraploid and Chimeric Plants from which Euploids could be Derived by Callus Induction

Barandalla¹,

Ritter²,

Galarreta³

2007

Potato Res.

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Shoot apices from six diploid potato genotypes for polyploidisation were treated with oryzalin and colchicine solutions at different concentrations and incubation times. In addition to chimeric plants, diploids, one octoploid and completely ploidy doubled plants (4x) were regenerated in these experiments. Tetraploid plants were obtained for three of the six genotypes treated with oryzalin, and no tetraploid plants were obtained after colchicine treatments of any of the genotypes. The best result of 43% tetraploid plants was achieved using 28.8 μM oryzalin and 24 h of incubation with the L37 genotype. Moreover, we determined that doubled plants should not be selected on the basis of a single measurement and require additional ploidy checks in successive clonal generations. Some L37 plants that were initially identified as tetraploids by flow cytometry produced diploid or mixoploid plants in the next clonal generation. Morphological characters of doubled L37 plants were compared with the original genotype. The PVY resistance present in a parental clone was maintained in all regenerants and significant differences among ploidy variants were found for some quantitative characters. Molecular AFLP analyses did not show any differences among genotypes. Since polyploidizing substances may produce a large proportion of chimeric plants, we applied a method to increase the number of tetraploid plants. Callus culture of chimeric leaf explants was performed on regeneration medium and tetraploid shoots were obtained in four of the six genotypes tested. The best result of 40% tetraploid plants was achieved with explants from the L37 genotype. This method was successful in producing additional tetraploid genotypes from mixoploid plants obtained after oryzalin treatments.

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Section: Introductionmentioning

confidence: 99%

Oryzalin Treatment of Potato Diploids Yields Tetraploid and Chimeric Plants from which Euploids could be Derived by Callus Induction

Barandalla¹,

Ritter²,

Galarreta³

2007

Potato Res.

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“…There are two main pathways by which haploid formation can be induced in potato: androgenesis and gynogenesis. Androgenesis is through in vitro culturing of whole anthers or free microspores on a nutrient rich medium to induce plantlet regeneration from single gametic cells or haploid calli (Veilleux 1996), while gynogenesis is haploidization via the "maternal" or seed parent's genome. Potato haploids are routinely obtained by gynogenesis, a process in which specific S. tuberosum Group Phureja (2n = 2x = 24) selections, known as "haploid inducers," contribute the paternal gametes for pollination of the desired haploid progenitor.…”

Section: Haploids and Disomic Inheritancementioning

confidence: 99%

Genetics and Cytogenetics of the Potato

Ortíz

Mihovilovich²

2020

The Potato Crop

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Tetraploid potato (Solanum tuberosum L.) is a genetically complex, polysomic tetraploid (2n = 4x = 48), highly heterozygous crop, which makes genetic research and utilization of potato wild relatives in breeding difficult. Notwithstanding, the potato reference genome, transcriptome, resequencing, and single nucleotide polymorphism (SNP) genotyping analysis provide new means for increasing the understanding of potato genetics and cytogenetics. An alternative approach based on the use of haploids (2n = 2x = 24) produced from tetraploid S. tuberosum along with available genomic tools have also provided means to get insights into natural mechanisms that take place within the genetic load and chromosomal architecture of tetraploid potatoes. This chapter gives an overview of potato genetic and cytogenetic research relevant to germplasm enhancement and breeding. The reader will encounter findings that open new doors to explore inbred line breeding in potato and strategic roads to access the diversity across the polyploid series of this crop's genetic resources. The text includes classical concepts and explains the foundations of potato genetics and mechanisms underlying natural cytogenetics phenomena as well as their breeding applications. Hopefully, this chapter will encourage further research that will lead to successfully develop broad-based potato breeding populations and derive highly heterozygous cultivars that meet the demands of having a resilient crop addressing the threats brought by climate change.

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“…Although S. tuberosum was ranked previously as a recalcitrant species due to a poor androgenic capacity (Veilleux 1996), we have obtained plants in genetically diverse potato material through gametic embryogenesis (Rokka et al 1996). Over 20 commercial cultivars have shown androgenic response with dihaploid green plant formation.…”

Section: Anther Culture or Pollination With Solanum Phurejamentioning

confidence: 99%