Haploids in Twin Seedlings of Pepper

Christensen, Hilde M.; Bamford, Ronald

doi:10.1093/oxfordjournals.jhered.a105264

Cited by 31 publications

(9 citation statements)

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“…Haploid and spontaneous diploid plant production from anther culture is a well-developed and useful tool in practical plant breeding. Spontaneous parthenogenesis was the first system used to obtain haploid plants in bell pepper (Christensen and Bamford, 1943) but the efficiency of production was low (Pochard and Dumas De Vaulx, 1979). Sibi et al (1979) introduced a more successful two step anther culture system, which was further optimized by Dumas de Vaulx et al (1981) which has been used to produce many doubled haploid plants of bell pepper for the breeding programs (Abak et al, 1982;Caranta et al, 1996;Dumas de Vaulx and Pochard, 1986;Pochard et al, 1983).…”

Section: Research Reportmentioning

confidence: 99%

Agro-morphological characterization of anther derived plants in sweet pepper (Capsicum annuum L. cv. Boogie)

Shrestha

Luitel

Kang

2011

Hortic. Environ. Biotechnol.

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This study was conducted in sweet pepper 'Boogie' to produce the haploids and diploids via anther culture, and to characterize their plant and fruit characters. Anthers were cultured on Dumas de Vaulx medium, (C-medium) supplemented with 0.1 mg L -1 kinetin and 0.1 mg L -1 2, 4-D, and 3% sucrose and 0.32% phytagel. Cultures were first incubated in dark at 35 for one week and then transferred at 25 under 16 h photoperiod for 40 days. Regenerated plantlets including haploids and diploids were evaluated for their plant and fruit characters at Hwacheon in spring, 2007. Results revealed that 39.6 % callus was formed in total anthers (6100) whereas only 1.78% plants were regenerated among them. Ploidy analysis showed that 78.1% regenerants were diploids. Haploid plants were characterized by short plant height, narrow leaf size, short internode length, small flower bud size, and small fruit with low volume than diploid plants. Average fruit volume measured in diploids was 62.7% higher than haploids. Variation in plant and fruit characters was observed within the diploid population and pepper breeder could utilize such variation in breeding program. Based on this characterization and compared to original cultivar, SP94, SP117, SP148, SP171 and SP174 are found superior diploid lines and recommended them to evaluate for variety improvement in sweet pepper.Additional key words: 2,4-D, callus, diploid, Dumas de Vaulx medium, haploid, kinetin Hort.

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Section: Research Reportmentioning

confidence: 99%

Agro-morphological characterization of anther derived plants in sweet pepper (Capsicum annuum L. cv. Boogie)

Shrestha

Luitel

Kang

2011

Hortic. Environ. Biotechnol.

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“…Spontaneous haploids have been found to occur in Capsicum species (Christensen and Bamford, 1943;Campos and Morgan, 1958;Pochard and Dumas de Vaulx, 1979). The haploids appear mainly in the form of twin seedlings, at rates ranging from 1 to 10 in 10 000 germinated seeds.…”

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confidence: 99%

In vitro chili pepper biotechnology

Ochoa-Alejo

Ramírez-Malagón

2001

In Vitro Cell.Dev.Biol.-Plant

121

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Chili pepper is an important horticultural crop that can surely benefit from plant biotechnology. However, although it is a Solanaceous member, developments in plant cell, tissue, and organ culture, as well as on plant genetic transformation, have lagged far behind those achieved for other members of the same family, such as tobacco (Nicotiana tabacum), tomato (Lycopersicon esculentum), and potato (Solanum tuberosum), species frequently used as model systems because of their facility to regenerate organs and eventually whole plants in vitro, and also for their ability to be genetically engineered by the currently available transformation methods. Capsicum members have been shown to be recalcitrant to differentiation and plant regeneration under in vitro conditions, which in turn makes it very difficult or inefficient to apply recombinant DNA technologies via genetic transformation aimed at genetic improvement against pests and diseases. Some approaches, however, have made possible the regeneration of chili pepper plants from in vitro-cultured cells, tissues, and organs through organogenesis or embryogenesis. Anther culture has been successfully applied to obtain haploid and doubledhaploid plants. Organogenic systems have been used for in vitro micropropagation as well as for genetic transformation. Application of both tissue culture and genetic transformation techniques have led to the development of chili pepper plants more resistant to at least one type of virus. Cell and tissue cultures have been applied successfully to the selection of variant cells exhibiting increased resistance to abiotic stresses, but no plants exhibiting the selected traits have been regenerated. Production of capsaicinoids, the hot principle of chili pepper fruits, by cells and callus tissues has been another area of intense research. The advances, limitations, and applications of chili pepper biotechnology are discussed.

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“…Although there is a large number of species with the chromosome number of 2n = 24, the basic number in this family appears to be six, and not twelve (Goodspeed, 1954;Love and Love, 1961). This is suggested from the experimental number 2n = 12 in the genera Capsicum (Christensen and Bamford, 1943), Datura (Satina et al, 1941), Lycopersicon (Rick, 1945) and Nicotiana (Webber, 1933). Thus it is possible that there has been a duplication of the S-bearing chromosome early in the evolution of this family, a conclusion which also is consistent with the finding of twolocus systems of incompatibility in certain species of the two genera, Physalis (Pandey, 1957) and Solanum (Pandey, 1960(Pandey, , 1962.…”

Section: S-gene Mutations and Duplication Of The S Locusmentioning

confidence: 93%

Elements of the S-gene complex III. Chromosome fragments and naturally occurring S-gene mutations in Nicotiana bonariensis

Pandey¹

1969

Heredity

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Haploids in Twin Seedlings of Pepper

Cited by 31 publications

References 0 publications

Agro-morphological characterization of anther derived plants in sweet pepper (Capsicum annuum L. cv. Boogie)

Agro-morphological characterization of anther derived plants in sweet pepper (Capsicum annuum L. cv. Boogie)

In vitro chili pepper biotechnology

Elements of the S-gene complex III. Chromosome fragments and naturally occurring S-gene mutations in Nicotiana bonariensis

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