Haploids in Genetically Marked Progenies of Tobacco

Burk, L. G.

doi:10.1093/oxfordjournals.jhered.a107176

Cited by 26 publications

(19 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Besides, most of these examples date from more than 40 years ago, with very scarce new contributions to the knowledge of this phenomenon in these four decades. In tobacco (Nicotiana tabacum) a mean rate of one androgenic haploid every 2,500 individuals was reported by Burk (1962). In maize (Zea mays), the highest mean frequency of spontaneous androgenesis reported is 1/80,000 in a sample size of 400,000 individuals from ordinary strains (Chase, 1969;Goodsell, 1961).…”

Section: Male-derived Haploid Embryogenesis In the Embryo Sacmentioning

confidence: 99%

Androgenesis Revisited

Seguí-Simarro

2010

Bot. Rev.

106

View full text Add to dashboard Cite

Androgenesis can be defined as the set of biological processes leading to an individual genetically coming exclusively from a male nucleus. Androgenesis was traditionally considered as the spontaneous, in vivo development of a male-derived haploid embryo from a fertilized egg where the female nucleus is eliminated. However, at present it is also possible to generate androgenic haploids/doubled haploids through in vitro microspore embryogenesis and by in vitro meiocyte-derived callogenesis. These three androgenic alternatives clearly differ in the inducible stage, but lead to the same final haploid or doubled haploid product. Whereas microspore embryogenesis is widely studied and applied, the other two routes are much less known. In this paper, the evidence accounting for the existence of these three alternative pathways is revised, as well as the mechanisms potentially involved in their induction. Their differences and similarities are discussed from a biological perspective, leading to the notion that they might represent an ancient survival mechanism triggered by similar factors. Resumen La androgénesis se define como el conjunto de vías biológicas que dan lugar a un individuo cuyo fondo genético proviene exclusivamente de un núcleo de origen masculino. Tradicionalmente, el concepto de androgénesis estaba restringido al desarrollo espontáneo, in vivo, de un embrión haploide o doble haploide a partir de una célula huevo fecundada en la que el material genético de origen femenino era inactivado o eliminado. Sin embargo, hoy en día sabemos que existen otras vías para conseguir haploides o doble haploides androgénicos, mediante embriogénesis de microsporas y mediante la formación de callos derivados de meiocitos. Estas tres alternativas androgénicas difieren claramente en la etapa en la que es posible la inducción, pero dan lugar al mismo producto haploide o doble haploide final. Mientras que la embriogénesis de microsporas es un fenómeno ampliamente estudiado y de clara aplicación práctica, las otras dos rutas son mucho menos conocidas. En este trabajo se revisan las evidencias existentes al respecto de estas tres alternativas androgén-icas, así como los mecanismos potencialmente implicados en su inducción. También se discuten sus diferencias y semejanzas, desde un punto de vista biológico, para llegar a la hipótesis de que estas rutas podrían representar un mecanismo atávico de supervivencia activado por factores similares.

show abstract

Section: Male-derived Haploid Embryogenesis In the Embryo Sacmentioning

confidence: 99%

Androgenesis Revisited

Seguí-Simarro

2010

Bot. Rev.

106

View full text Add to dashboard Cite

show abstract

“…Introduction of deleterious variation using this process (reviewed by Wernsman 1992) has limited the utility of this method in practical breeding programs, however. Haploid plants of both maternal and paternal origin occur spontaneously at low frequencies in progeny of N. tabacum 9 N. tabacum crosses (de Nettancourt and Stokes 1960;Burk 1962;Lewis and Rose 2011). Burk et al (1979) found that N. tabacum could also yield low frequencies of maternal haploids when pollinated with the African species N. africana [Merx.…”

Section: Introductionmentioning

confidence: 99%

“…Methods previously used for tobacco involve a recessive yellow seedling marker (yg) (Burk 1962); the TMV-resistance gene, N (de Nettancourt and Stokes 1960); a rootless Racmutant genetic stock (Pelletier et al 1987); and a transgenic purple seedling trait (Lewis and Rose 2011). Some of these visible markers are not useful in applied breeding programs, however, because of the required genetics of elite cultivars.…”

Section: Introductionmentioning

confidence: 99%

Identification of maternal haploids of Nicotiana tabacum aided by transgenic expression of green fluorescent protein: evidence for chromosome elimination in the N. tabacum × N. africana interspecific cross

et al. 2015

View full text Add to dashboard Cite

The Nicotiana tabacum 9 N. africana cross is a semilethal cross, where greater than 99 % of progeny die in the cotyledonary stage due to an interspecific lethality reaction. The low frequencies of surviving plants are mixtures of maternal tobacco haploids and aneuploid F 1 hybrids. For effective use of surviving haploids in a breeding program, an efficient method is needed to distinguish them from aneuploid hybrids during the seedling stage. The first objective of this research was to investigate the use of N. africana engineered with a green fluorescent protein (gfp) transgene for distinguishing haploids from other surviving plants derived from this cross. Results demonstrated gfp expression to be a useful phenotypic marker for separation of aneuploid hybrids at the seedling stage. Microsatellite marker genotyping, flow cytometry determinations, and chromosome counts resulted in identification of progeny that exhibited intermediate DNA amounts and variable chromosome numbers donated by the N. africana parent. Three individuals with near-haploid DNA content were identified that carried N. africana markers and n = 24 ? 2 or n = 24 ? 3 chromosomes. The results present evidence of genetic instability in progeny from the N. tabacum 9 N. africana cross and suggest that, in contrast to previous belief, chromosome elimination may play at least a partial role in the generation of haploids from this cross. Additionally, microsatellite marker genotyping demonstrated a role for a gene or genes located near the end of N. tabacum chromosome H (linkage group 11) in the interspecific lethality reaction.

show abstract

“…Firstly, this spontaneous phenomenon has been reported in B. oleracea (Thompson, 1956). Secondly, in species where both spontaneous gynogenetic and androgenetic plants were recovered, as in tobacco (Burk, 1962) or in maize (Chase, 1969), the frequency of gynogenetic plants was generally higher than the frequency of androgenetic plants.…”

Section: Discussionmentioning

confidence: 76%

Potential use of theaux2 gene fromAgrobacterium rhizogenes as a conditional negative marker in transgenic cabbage

Béclin

Charlot

Botton

et al. 1993

Transgenic Research

View full text Add to dashboard Cite

An original Agrobacterium tumefaciens-mediated transformation procedure, based on the actions of both wild type and disarmed bacterial strains, was developed. The aux2 gene of A. rhizogenes was introduced into a rapid-cycling genotype of cabbage (Brassica oleracea L.). The aux2 gene product converts naphthalene acetamide into the auxin naphthalene acetic acid. Expression of this gene in the transgenic progeny grown in vitro led to an altered root phenotype. On a medium supplemented with napthalene acetamide (NAM), two of the three analysed progenies were characterized by the formation of callus instead of roots, whereas on a NAM-free medium all the planflets from these progenies presented a normal phenotype. Expression of the aux2 gene was also assessed under horticultural conditions by sowing seeds in sand and watering them with a nutritive solution supplemented with NAM. Under these conditions, NAM inhibited the formation of a root system in transgenic plantlets and induced the death of the transgenic plantlets three to four weeks after germination. Thus, aux2 acts as a lethal conditional marker which could be used in negative selection of cabbage. Potential utilization of the aux2 gene to screen spontaneous androgenetic plants in order to transfer cytoplasmic male sterility in a single generation is discussed.

show abstract

Haploids in Genetically Marked Progenies of Tobacco

Cited by 26 publications

References 0 publications

Androgenesis Revisited

Androgenesis Revisited

Identification of maternal haploids of Nicotiana tabacum aided by transgenic expression of green fluorescent protein: evidence for chromosome elimination in the N. tabacum × N. africana interspecific cross

Potential use of theaux2 gene fromAgrobacterium rhizogenes as a conditional negative marker in transgenic cabbage

Contact Info

Product

Resources

About