Haploidization in<i>Saccharomyces cerevisiae</i>Induced by a Deficiency in Homologous Recombination

Song, Wei; Petes, Thomas D.

doi:10.1534/genetics.111.138180

Cited by 16 publications

(15 citation statements)

References 22 publications

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“…Several S. cerevisiae mutantions have been identified that lead to genome reduction from diploid to haploid. For example, diploid cells with a null mutation in RAD52 (involved in strand exchange during recombination and DNA damage repair), undergo chromosome loss via sequential aneuploid transitions (87). Loss of Rad52 leads to a failure of the repair mechanism that keeps the chromosome homologs together after a double strand break (DSB).…”

Section: Mutations Underlying Ploidy Reductionmentioning

confidence: 99%

Ploidy Variation in Fungi: Polyploidy, Aneuploidy, and Genome Evolution

2017

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The ability of an organism to replicate and segregate its genome with high fidelity is vital to its survival and for the production of future generations. Errors in either of these steps (replication or segregation) can lead to a change in ploidy or chromosome number. While these drastic genome changes can be detrimental to the organism, resulting in decreased fitness, they can also provide increased fitness during periods of stress. A change in ploidy or chromosome number can fundamentally change how a cell senses and responds to its environment. Here, we discuss current ideas within fungal biology that illuminate how eukaryotic genome size variation can impact the organism at a cellular and evolutionary level. One of the most fascinating observations from the last two decades of research is that some fungi have evolved the ability to tolerate large genome size changes and generate vast genomic heterogeneity without undergoing canonical meiosis.

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Section: Mutations Underlying Ploidy Reductionmentioning

confidence: 99%

Ploidy Variation in Fungi: Polyploidy, Aneuploidy, and Genome Evolution

2017

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“…An advanced form a-CGH-based method involves the use of single nucleotide polymorphisms (SNP) array. It is a rapid, high-resolution, and cost-effective tool used for the characterization of changes in allele diversity as well as chromosome copy number in C. albicans and also in S. cerevisiae (Song & Petes, 2012).…”

Section: A-cghmentioning

confidence: 99%

Yeast: a simple model system to study complex phenomena of aneuploidy

Mulla

Zhu

2014

FEMS Microbiol Rev

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Aneuploidy, the state of having a chromosome number different from a multiple of the haploid number, has been associated with diseases and developmental disorders. The role of aneuploidy in human disease pathology, especially in cancer, has been a subject of much attention and debate over the last century due to the intrinsic complexity of the phenomena and experimental challenges. Over the last decade, yeast has been an invaluable model for driving discoveries about the genetic and molecular aspects of aneuploidy. The understanding of aneuploidy has been significantly improved owing to the methods for selectively generating aneuploid yeast strains without causing other genetic changes, techniques for detecting aneuploidy, and cutting-edge genetics and ‘omics’ approaches. In this review, we discuss the contribution of studies in yeast to current knowledge about aneuploidy. Special emphasis is placed on experimental features which make yeast a simpler and efficient model to investigate the complex questions in the field of aneuploidy.

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“…Saccharomyces cerevisiae strains that lack HR are very sensitive to DNA-damaging agents such as X-rays (Resnick and Martin 1976), and have a high rate of spontaneous chromosome loss (Song and Petes 2012). HR is essential for the survival of mammalian cells (Helleday 2003).…”

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confidence: 99%

Properties of Mitotic and Meiotic Recombination in the Tandemly-Repeated CUP1 Gene Cluster in the Yeast Saccharomyces cerevisiae

et al. 2017

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In the yeast Saccharomyces cerevisiae, the genes encoding the metallothionein protein Cup1 are located in a tandem array on chromosome VIII. Using a diploid strain that is heterozygous for an insertion of a selectable marker (URA3) within this tandem array, and heterozygous for markers flanking the array, we measured interhomolog recombination and intra/sister chromatid exchange in the CUP1 locus. The rate of intra/sister chromatid recombination exceeded the rate of interhomolog recombination by >10-fold. Loss of the Rad51 and Rad52 proteins, required for most interhomolog recombination, led to a relatively small reduction of recombination in the CUP1 array. Although interhomolog mitotic recombination in the CUP1 locus is elevated relative to the average genomic region, we found that interhomolog meiotic recombination in the array is reduced compared to most regions. Lastly, we showed that high levels of copper (previously shown to elevate CUP1 transcription) lead to a substantial elevation in rate of both interhomolog and intra/sister chromatid recombination in the CUP1 array; recombination events that delete the URA3 insertion from the CUP1 array occur at a rate of >10−3/division in unselected cells. This rate is almost three orders of magnitude higher than observed for mitotic recombination events involving single-copy genes. In summary, our study shows that some of the basic properties of recombination differ considerably between single-copy and tandemly-repeated genes.

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Haploidization inSaccharomyces cerevisiaeInduced by a Deficiency in Homologous Recombination

Cited by 16 publications

References 22 publications

Ploidy Variation in Fungi: Polyploidy, Aneuploidy, and Genome Evolution

Ploidy Variation in Fungi: Polyploidy, Aneuploidy, and Genome Evolution

Yeast: a simple model system to study complex phenomena of aneuploidy

Properties of Mitotic and Meiotic Recombination in the Tandemly-Repeated CUP1 Gene Cluster in the Yeast Saccharomyces cerevisiae

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