Haploid Plants from Tissue Culture: New Plant Varieties in a Shortened Time Frame

Cytological Investigation of R1 and R2-Generations of Spontaneously and Colchicine Induced Diploid Anther Derived Wheat Plants.

“…However, this aspect-compared to the technique itself received minor attention (Henry and De Buyser 1990), although genetic variation and chromosomal instability could be induced through anther or microscope culture in wheat and other members of Gramineae leading to deleterious effects on the agronomic performance, homogeneity, and genetic stability of obtained DH-plants (for rev. Morrison and Evans 1988). In the present investigation, the results obtained in Table 1, which showed a significant increase of meiotic abnormalities in regenerated plants of anther culture in comparison to the parental anther donor (control) confirmed the role of in vitro conditions in disturbing pairing and evoking meiotic irregularities (Lee and Phillips 1987, Metz et al 1988, Henry and De Buyser 1990, Wang and Hu 1993.…”

“…It is agreed upon that in vitro plant cell culture may lead to genetic and cytogenetic modifications in regenerated plants, collectively known as somaclonal variations in case of using somatic tissue (Larkin and Scrowcroft 1981) and gametoclonal variations in case of using gametophytic cells (Evans et al 1984, Lee and Phillips 1988, Evans 1989. Anther and microsporocyte culture in Gramineae is expected to show such gametoclonal variations, in particular if associated with a callus phase (Morrison and Evans 1988). Incidence of genetic variations is frequently of deleterious nature and represent sometimes a serious obstacle for a successful application of anther derived doubled haploid (DH) plants in crop improvement (Finnie et al 1991).…”

Plant Tissue Culture: Current Status and Opportunities

“…The tissue culture techniques enable to produce homozygous plants in relatively short time period through the protoplast, anther and microspore cultures instead of conventional breeding [53].…”

In vitro Haploid Plantlet Regeneration through Anther Culture in Locally Adapted Cultivar of Indian Mustard (Brassica juncea L. Czern and Coss)

“…Traditionally, homozygosity of the cross products is usually achieved by self-fertilization which is a time consuming process (Morrison and Evans, 1988).…”