Haploid Plant Induction in Zucchini (Cucurbita Pepo L. Convar. Giromontiina Duch) and in Cucumber (Cucumis Sativus L.) Lines Through in Vitro Gynogenesis

Juhász, Anikó Gémesné; Venczel, G.; Balogh, Péter

doi:10.17660/actahortic.1997.447.124

Cited by 13 publications

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“…In Cucurbitaceae species, haploid plants have been obtained by gynogenesis and parthenogenesis (Claveria et al, 2005;Gémesné-Juhász et al, 1997;Niemirowicz-Szczytt and Dumas de Vaulx, 1989;Przyborowski and Niemirowicz-Szczytt, 1994;Sari et al, 1994;Sauton, 1989;Yanmaz et al, 1999). In melon, the first haploids were obtained by intraspecific crosses (Dumas de Vaulx, 1979).…”

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confidence: 99%

Parthenogenic Haploids in Melon: Generation and Molecular Characterization of a Doubled Haploid Line Population

Gonzalo¹,

Claveria²,

Monforte³

et al. 2011

J. Amer. Soc. Hort. Sci.

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Melon (Cucumis melo) is one of the principal vegetable crops for fresh market, for which a large number of breeding programs, oriented to generate inbred pure lines and hybrids, is established worldwide. The process to obtain and select these lines has been highly accelerated by the use of biotechnological techniques such as the generation of doubled haploid line (DHL) populations and molecular markers. Moreover, the use of DHLs in genetic studies is a useful tool because of their complete homozygosity and the permanent availability of plant material perpetuated by seed. In this work, the parthenogenetic response of 17 melon genotypes and the F1 hybrid PI 161375 × Spanish cultivar Piel de Sapo (PS) was studied considering three stages along the in vitro DHL generation process. The response of the analyzed melon cultivars was heterogeneous through the DHL generation with different limiting steps for each genotype. The response of the PI 161375 × PS hybrid was more similar to the male (PS) than the female parent (PI 161375), although the response of the maternal genotype was higher for some stages. This points to the important role of alleles from both parents in the different steps of the DHL generation process, and it could explain the identification of six genomic regions with distorted allelic segregation skewed toward PS or PI 161375. This hybrid was used to generate a population of 109 DHLs, the gametophytic origin of which was confirmed by flow cytometry and molecular markers.

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confidence: 99%

Parthenogenic Haploids in Melon: Generation and Molecular Characterization of a Doubled Haploid Line Population

Gonzalo¹,

Claveria²,

Monforte³

et al. 2011

J. Amer. Soc. Hort. Sci.

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“…The application of gynogenesis (Dirks, 1996;Gémesné-Juhász et al, 1997) and androgenesis (Kumar et al, 2003), through in vitro ovary and anther culture, were described as alternative but not more effi cient approaches to generate haploids in cucumber than parthenogenesis. These three reports measured the production of haploid plants but gave no details about the recovery of fertile DHLs, essential for the use of these techniques in breeding programs.…”

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confidence: 99%

Optimization of Cucumber Doubled Haploid Line Production Using In Vitro Rescue of In Vivo Induced Parthenogenic Embryos

Claveria¹,

García-Más²,

Dolcet-Sanjuan³

2005

jashs

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ADDITIONAL INDEX WORDS. Cucumis sativus, Cucurbitaceae, parthenogenesis, haploid, DHL, pure line, hybrid ABSTRACT. Homozygous doubled haploid lines (DHLs) from new cucumber (Cucumis sativus L.) accessions could be useful to accelerate breeding for resistant varieties. DHLs have been generated by in vitro rescue of in vivo induced parthenogenic embryos. The protocol developed involves the following: 1) induction of parthenogenic embryos by pollinating with pollen irradiated with a Co 60 γ-ray source at 500 Gy; 2) in vitro rescue of putative parthenogenic embryos identifi ed by their morphology and localized using a dissecting scope or X-ray radiography; 3) discrimination of undesirable zygotic individuals from the homozygous plants using cucumber and melon SSR markers; 4) determination of ploidy level from homozygous plants by fl ow cytometry; 5) in vitro chromosome doubling of haploids; and 6) acclimation and selfi ng of selected lines. Codominant markers and fl ow cytometry confi rmed the gametophytic origin of plants regenerated by parthenogenesis, since all homozygous lines were haploids. No spontaneous doubled haploid plants were rescued. Chromosome doubling of haploid plants was accomplished by an in vitro treatment with 500 μM colchicine. Rescue of diploid or chimeric plants was shown by fl ow cytometry, prior to their acclimation and planting in the greenhouse. Selfi ng of colchicine-treated haploid plants allowed for the perpetuation by seed of homozygous lines. The high rate of seed set, 90% of the lines produced seed, facilitated the recovery of inbred lines. Despite some limiting factors, parthenogenesis is routinely used in a cucumber-breeding program to achieve complete homozygosity in one generation. Breeding for new commercial hybrid cultivars will be accelerated. DHLs are ideal resources for genomic analyses.

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“…Работы по индукции гаплоидии при культивировании неопыленных завязей/семяпочек в дальнейшем продолжились и на других культурах семейства Cucurbitaceae [42][43][44][45][46].…”

Section: история открытия гаплоидов и первые гаплоиды у культур семейства Cucurbitaceaeunclassified

Obtaining doubled haploids of Cucurbita pepo L.

2021

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Doubled haploids have been widely used worldwide in breeding programs and fundamental research as valuable homozygous material for about 100 years. The species Cucurbita pepo L. are represented by a huge variety of forms, include highly productive vegetable crops and have a wide distribution in the world. Despite the great economic importance, the creation of effective protocols to ensure stable production of doubled haploids in this species remains an urgent task. DH plants are of interest not only because of the acceleration of the breeding process, but also because of the realization of the huge potential of gametoclonal variability inherent in this highly polymorphic species. In this review, we analyzed the main technologies used for obtaining doubled haploids in vegetable crops of C. pepo: parthenogenesis in situ stimulated by treated/irradiated pollen, gynogenesis in vitro (unpollinated ovule culture in vitro) and androgenesis in vitro (anther/microspore culture in vitro). An analysis is presented of the research carried out from the beginning of the discovery of haploid plants to the current advances and evaluation of the prospects in the field of DH plant production. The main critical factors influencing the efficiency of each technology and its individual steps are considered. The developed technology of doubled haploids obtaining using non-pollinated ovary culture in vitro is presented. This technology allows to obtain up to 55 embryoids per one cultivated ovary (28 embryoids/ 100 cultivated ovules) To introduce haploid technologies into the breeding process it is necessary to evaluate the obtained plants for ploidy level. The use of direct counting of chromosomes in apical cells may present a certain difficulty in this species due to their large number (2n=40) and their small size. Depending on the level of laboratory equipment, ploidy determination using flow cytometry of cell nuclei and counting the number of chloroplasts in stomatal guard cells in the epidermis of the abaxial side of the leaf may be more convenient methods. The prospects for the use of molecular markers for assessment for homozygosity in DH technologies used, including C. pepo, are discussed in the review.

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Haploid Plant Induction in Zucchini (Cucurbita Pepo L. Convar. Giromontiina Duch) and in Cucumber (Cucumis Sativus L.) Lines Through in Vitro Gynogenesis

Cited by 13 publications

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Parthenogenic Haploids in Melon: Generation and Molecular Characterization of a Doubled Haploid Line Population

Parthenogenic Haploids in Melon: Generation and Molecular Characterization of a Doubled Haploid Line Population

Optimization of Cucumber Doubled Haploid Line Production Using In Vitro Rescue of In Vivo Induced Parthenogenic Embryos

Obtaining doubled haploids of Cucurbita pepo L.

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