Haploid Oat Plants Produced by Application of Maize Pollen to Emasculated Oat Florets

Rines, H. W.; Dahleen, Lynn S.

doi:10.2135/cropsci1990.0011183x003000050023x

Cited by 82 publications

(43 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cross between oat and maize, two species from different subfamilies of the Graminae, represents the widest combination of crop species to date yielding stable, fertile partial hybrids (37). However, little is known about the comparative structure and composition of the two genomes.…”

Section: Discussionmentioning

confidence: 99%

Oat–maize chromosome addition lines: A new system for mapping the maize genome

Ananiev

Riera‐Lizarazu

Rines

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Novel plants with individual maize chromosomes added to a complete oat genome have been recovered via embryo rescue from oat (Avena sativa L., 2n ‫؍‬ 6x ‫؍‬ 42) ؋ maize (Zea mays L., 2n ‫؍‬ 20) crosses. An oat-maize disomic addition line possessing 21 pairs of oat chromosomes and one maize chromosome 9 pair was used to construct a cosmid library. A multiprobe (mixture of labeled fragments used as a probe) of highly repetitive maize-specific sequences was used to selectively isolate cosmid clones containing maize genomic DNA. Hybridization of individual maize cosmid clones or their subcloned fragments to maize and oat genomic DNA revealed that most high, middle, or low copy number DNA sequences are maize-specific. Such DNA markers allow the identification of maize genomic DNA in an oat genomic background. Chimeric cosmid clones were not found; apparently, significant exchanges of genetic material had not occurred between the maize-addition chromosome and the oat genome in these novel plants or in the cloning process. About 95% of clones selected at random from a maize genomic cosmid library could be detected by the multiprobe. The ability to selectively detect maize sequences in an oat background enables us to consider oat as a host for the cloning of specific maize chromosomes or maize chromosome segments. Introgressing maize chromosome segments into the oat genome via irradiation should allow the construction of a library of overlapping fragments for each maize chromosome to be used for developing a physical map of the maize genome.

show abstract

Section: Discussionmentioning

confidence: 99%

Oat–maize chromosome addition lines: A new system for mapping the maize genome

Ananiev

Riera‐Lizarazu

Rines

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Albino plants create a big problem for the androgenesis method in all cereals, including oat [15]. The wide crossing method (chromosomes elimination) seems to be more effective than androgenesis, and moreover, there are no albino plants among the regenerants [16]. Until now there have only been a few publications concerned with obtaining oat haploids using wide crossing.…”

Section: Introductionmentioning

confidence: 99%

“…Until now there have only been a few publications concerned with obtaining oat haploids using wide crossing. In one of the first experiments using this method, 14 haploid plants were obtained when approximately 3300 florets were pollinated with maize pollen [16]. Matzk [17] indicates that obtaining oat haploid embryos is possible if the florets are pollinated with maize, pearl millet Pennisetum Americanum (L.) Leeke and eastern gamagrass Tripsacum dactyloides (L.) L. The efficiency of his experiments was less than 0.1% due to poor regeneration.…”

Section: Introductionmentioning

confidence: 99%

Production of double haploids in oat (Avena sativa L.) by pollination with maize (Zea mays L.)

et al. 2013

View full text Add to dashboard Cite

Production of double haploids in oat (Avena sativa L.) by pollination with maize (Zea mays L.) Abbreviations 2,4-D -2,4-dichlorophenosyacetic acid; dicamba -3,6-dichloro-2-methoxybenzoic acid; DH -double haploid. IntroductionModern crop breeding has a number of advantages when using DH plants, such as shortening of the production time of new varieties and the possibility of using them in research. Classical methods of production of new varieties, based on a selection in every generation, take six to ten years. Biotechnological methods allow for a shortening of this time, even to one vegetative season. Breeders can make new cultivars with features from DH lines and be confident about completely homozygous plants. Moreover, DH lines can be used in the production of genetic maps, which help in the search for quantitative traits loci (QTL), conjugated with molecular markers and connected with many physiological processes, such as receptivity to the effectiveness of androgenesis or tolerance to biotic and abiotic stresses [1,2]. Haploids can be obtained using a number of methods, namely: from male gametophyte by androgenesis, using anther cultures or isolated microspores cultures [3][4][5][6][7][8], or from female gametophytes by gynogenesis or wide crossing, usually with maize (a method also known as chromosomes elimination) [1,9,10]. In comparison to anther culture, pollination with maize has an advantage because all regenerated haploid embryos are green, whereas in anther culture many of them are albino. The method of chromosome elimination that has been used in these experiments includes the following stages: after emasculation of cereal flowers the stigma is pollinated by maize, the pollen then germinates, a pollen tube is formed [11,12] and grows into the cereal embryo sac, where the cereal egg is fertilised by the maize sperm nuclei. A hybrid zygote is produced. The hybrid zygotes Keywords: Wide crossing • 2,4-D • Dicamba • Oat haploid embryosAbstract: The aim of the study was to optimize the method of oat haploid production by pollination with maize. Seventeen oat genotypes were used in the experiment. Various factors influencing the growth and development of ovaries and embryo production were investigated: genotype, time of pollination, growth regulators and time of their application. Emasculated before anthesis, oat florets were pollinated with maize pollen after 0, 1 or 2 days. Next, one of two auxins analogues (2,4-D or dicamba) were applied to oat pistils. These auxins had no significant influence on the number of enlarged ovaries and embryos. The time of application of these growth regulators had a significant influence on embryo production. Haploid embryos were obtained from all used genotypes, although the frequency of enlarged ovaries and obtained embryos did not differ markedly between the genotypes. On average, 85% of ovaries were enlarged and 11.7% of them produced haploid embryos. Depending on the regeneration medium, 24-41% of embryos were germinated, of which 12% had developed into green plant...

show abstract

“…Chromosome elimination has been documented in other interspecific or intergeneric crosses for doubled haploid production (Singh 1993), i.e., barley × Hordeum bulbosum (Kasha and Kao 1970;Subrahmanyam and Kasha 1973), wheat × H. bulbosum (Miller et al 1983), wheat × maize (Laurie and Bennett 1989), wheat × pearl millet (Laurie 1989) and oat × maize (Rines and Dahleen 1990). However, in these cases, chromosome elimination occured as the F 1 hybrid was formed, rather than after the F 1 hybrid had developed, as was the case in this study.…”

Section: Mechanisms Of Chromosome Doubling and Eliminationmentioning

confidence: 49%

Interspecific hybridization between diploid Fagopyrum esculentum and tetraploid F. homotropicum

Wang¹,

Scarth²,

Campbell³

2005

Can. J. Plant Sci.

View full text Add to dashboard Cite

. 2005. Interspecific hybridization between diploid Fagopyrum esculentum and tetraploid F. homotropicum. Can. J. Plant Sci. 85: 41-48. The wild diploid species Fagopyrum homotropicum (2n = 2x = 16) has been used for buckwheat improvement, but, prior to this study, the tetraploid form (2n = 4x = 32) had not been hybridized with the cultivated species F. esculentum. The objective of this study was to hybridize F. esculentum with tetraploid F. homotropicum to increase the genetic variability. Forty-one interspecific F 1 hybrids were obtained through ovule rescue in vitro, with hybridity confirmed using morphological characters, chromosome numbers (2n = 3x = 24) and DNA analysis. The F 1 plants were mainly sterile. However, seven seeds were set spontaneously on two hybrid plants, and a large number of seeds were obtained after colchicine treatment. The F 2 plants were divided into two groups based on chromosome numbers and morphology. The first group was hexaploid plants (2n = 6x = 48) or hypohexaploid plants (2n = 44-46), partially fertile with "gigas" features including increased height, dark green leaves, and large seeds with thick seed hulls. The second group of plants was diploid (2n = 2x = 16) (one plant had 17 chromosomes), with normal growth and fertility, and a combination of characters from both parents, indicating that genetic recombination had occurred during chromosome elimination. The diploid group was superior to the hexaploid group for use in buckwheat breeding programs due to the desirable characters and the ease of crossing. This is the first report of interspecific hybridization using different ploidy levels in the Fagopyrum genus.

show abstract

Haploid Oat Plants Produced by Application of Maize Pollen to Emasculated Oat Florets

Cited by 82 publications

References 0 publications

Oat–maize chromosome addition lines: A new system for mapping the maize genome

Oat–maize chromosome addition lines: A new system for mapping the maize genome

Production of double haploids in oat (Avena sativa L.) by pollination with maize (Zea mays L.)

Interspecific hybridization between diploid Fagopyrum esculentum and tetraploid F. homotropicum

Contact Info

Product

Resources

About