Haplodiploidization of maize (Zea mays L) through induced gynogenesis assisted by glossy markers and its use in breeding

Bordes, Jacques; Vaulx, Robert Dumas de; Lapierre, A.; Pollacsek, M.

doi:10.1051/agro:19970504

Cited by 33 publications

(26 citation statements)

References 7 publications

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“…Most crop species, however, have considerably longer generation times. Therefore, the creation of fully homozygous genotypes in one step via doubled-haploid (DH) induction ( Jensen 1975;Bajaj 1977;Bordes et al 1997;Wenzel et al 1977) is an interesting alternative to repeated self-pollination and, thus, was examined in my study (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Mating Designs for Establishing Nested Association Mapping Populations in Maize and Arabidopsis thaliana

Stich

2009

Genetics

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The nested association mapping (NAM) strategy promises to combine the advantages of linkage mapping and association mapping. The objectives of my research were to (i) investigate by computer simulations the power and type I error rate for detecting quantitative trait loci (QTL) with additive effects using recombinant inbred line (RIL) populations of maize derived from various mating designs, (ii) compare these estimates to those obtained for RIL populations of Arabidopsis thaliana, (iii) examine for both species the optimum number of inbreds used as parents of the NAM populations, and (iv) provide on the basis of the results of these two model species a general guideline for the design of NAM populations in other plant species. The computer simulations were based on empirical data of a set of 26 diverse maize inbred lines and a set of 20 A. thaliana inbreds both representing a large part of the genetic diversity of the corresponding species. I observed considerable differences in the power for QTL detection between NAM populations of the same size but created on the basis of different crossing schemes. This finding illustrated the potential to improve the power for QTL detection without increasing the total resources necessary for a QTL mapping experiment. Furthermore, my results clearly indicated that it is advantageous to create NAM populations from a large number of parental inbreds. M ANY traits that are important for fitness and agricultural value of plants are quantitative traits. Such traits are affected by many genes, the environment, and interactions between genes and the environment (Holland 2007). In plants, quantitative trait locus (QTL) mapping is a key tool for studying the genetic architecture of quantitative traits (Yano 2001). This method enables the estimation of (i) the number of genome regions affecting a trait, (ii) the distribution of gene effects, and (iii) the relative importance of additive and nonadditive gene action.Until now, most of the plant QTL mapping studies have been based on linkage mapping methods using individual biparental populations. The major limitations of such approaches are a poor resolution in detecting QTL and that with biparental crosses of inbred lines only two alleles at any given locus can be studied simultaneously (Flint-Garcia et al. 2005). Association mapping methods, which are successfully applied in human genetics to detect genes coding for human diseases (e.g., Willer et al. 2008), promise to overcome these limitations (Kraakman et al. 2004). However, in comparison with linkage mapping approaches, association mapping approaches have only a low power to detect QTL in genomewide scans (Yu and Buckler 2006).The nested association mapping (NAM) strategy proposed by Yu et al. (2008) uses recombinant inbred line (RIL) populations derived from several crosses of parental inbreds. Due to diminishing chances of recombination over short genetic distance and a given number of generations, the genomes of these RILs are mosaics of chromosomal segments of their p...

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Section: Discussionmentioning

confidence: 99%

Comparison of Mating Designs for Establishing Nested Association Mapping Populations in Maize and Arabidopsis thaliana

Stich

2009

Genetics

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“…Gynogenically derived haploid plants can be obtained in monocotyledonous species such as onion (Bohanec and Jakse 1999), leek (Schum et al 1993), shallot (Cohat 1994) and maize (Bordes et al 1997) as well as in dicotyledonous species such as sugarbeet (Ferrant and Bouharmont 1994), sunflower (Gelebart and San 1987) and gerbera (Tosca et al 1995). However, only a few results have been published on haploid induction in Cucurbitaceae.…”

Section: Introductionmentioning

confidence: 99%

Effect of optimal stage of female gametophyte and heat treatment on in vitro gynogenesis induction in cucumber ( Cucumis sativus L.)

Gémes-Juhász¹,

Balogh

Ferenczy

et al. 2002

Plant Cell Reports

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Gynogenic plants of pickling cucumber were successfully produced by in vitro culture from unpollinated ovules. Haploids and spontaneous doubled haploids originated directly through embryogenesis. The cucumber ovaries were placed on induction media containing thidiazuron and cultured in the dark for 2-5 days, at 24°C, 28°C or 35°C. After induction, the material was transferred to regeneration media containing α-naphthaleneacetic acid and 6-benzylaminopurine. Results from the regeneration test indicated that the heat treatment increased the efficiency of gynogenesis in the optimal developmental stage of the female gametophyte. The highest number of embryos occurred following the35°C induction treatment. The maximum frequency of gynogenesis was 18.4%, while maximum plant regeneration was 7.1%. The flow cytometry analysis showed that 87.7% of the regenerants were haploid. On the basis of histological studies carried out on the female gametophyte, the best developmental stage for haploid induction seems to be the cellularization stage of embryo-sac formation, when the nuclei are already in position, the membranes have sometimes developed, and the cells are quite uniform in shape and structure.

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“…A broad-base random mating population was developed by crossing 28 of these lines. Then forty eight DH lines were derived from this population and intercrossed to develop new population (Bordes et al 1997). Plants from the population were self-fertilized to produce 150 S 1 families.…”

Section: Development and Evaluation Of Genetic Materialsmentioning

confidence: 99%

“…The average haploid induction rate of FIGH1 was about 1%. Chromosome doubling was induced by colchicine treatment at the three ligulate leaf stage (Bordes et al 1997). After chromosome doubling, 261 DH lines were obtained from only 94 S 1 families with 1 to 8 DH lines per S 1 (22 S 1 with 1 DH, 31 with 2, 15 with 3, 11 with 4, 7 with 5, 4 with 6, 3 with 7, 1 with 8).…”

Section: Development and Evaluation Of Genetic Materialsmentioning

confidence: 99%

“…However, the rate of haploids obtained with Stock 6 and w 23 ig was still too low and depending on the genotype . Since these pioneering works, progress made in the in situ gynogenesis technique now allows production of a high number of DH for any genotype Chalyk 1994;Deimling et al 1997;Bordes et al, 1997). Doubled haploids (DH) may thus be useful in different breeding procedures used in maize improvement, pedigree selection and recurrent selection.…”

Section: Introductionmentioning

confidence: 99%

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Doubled-haploid versus single-seed descent and S1-family variation for testcross performance in a maize population

Bordes¹,

Charmet²,

Vaulx³

et al. 2006

Euphytica

Self Cite

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Progress made in the in situ gynogenesis technique since 1990 now allows production of a high number of maize (Zea mays L.) doubled-haploid (DH) lines. The aim of the study was to compare DH lines versus selfing lines for testcross performance. DH and single-seed descent (SSD) lines were produced from random S 1 progenies of a broad-base population. For grain yield, kernel moisture, plant height, ear height and leaf length, the three population means were similar. Except for kernel moisture, the genetic variance of DH lines was nearly twice as high as the genetic variance of S 1 families, as expected. On the other hand, genetic variance among SSD lines was only 1.5 times higher than the genetic variance of S 1 families. This lower variance could be due to a selection bias in the method of production of SSD lines. However, for all traits, heritability of SSD or DH lines was higher than heritability of S 1 families. Epistasis effects in DH progenies were not significant. The consequence was a high correlation between S 1 testcross progenies and DH or SSD testcross progenies, meaning that the S 1 testcross value can be used to select the best families from which DH lines will be extracted. As a whole, the observed variation in DH lines appeared to be more in accordance with the observed variation among S 1 families than with the observed variation among SSD lines.

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Haplodiploidization of maize (Zea mays L) through induced gynogenesis assisted by glossy markers and its use in breeding

Cited by 33 publications

References 7 publications

Comparison of Mating Designs for Establishing Nested Association Mapping Populations in Maize and Arabidopsis thaliana

Comparison of Mating Designs for Establishing Nested Association Mapping Populations in Maize and Arabidopsis thaliana

Effect of optimal stage of female gametophyte and heat treatment on in vitro gynogenesis induction in cucumber ( Cucumis sativus L.)

Doubled-haploid versus single-seed descent and S1-family variation for testcross performance in a maize population

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