Hands-on with optical tweezers: a multitouch interface for holographic optical trapping

Grieve, James A.; Ulčinas, Artūras; Subramanian, Sriram; Gibson, Graham M.; Padgett, Miles J.; Carberry, David M.; Miles, Mervyn J

doi:10.1364/oe.17.003595

Cited by 53 publications

(39 citation statements)

References 17 publications

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“…Other methods of particle translations, not directly applicable to our setup, include ones that are based on holographic techniques (for a recent, sophisticated method see Ref. [21]. )…”

Section: Methodsmentioning

confidence: 99%

Three-dimensional arrays of submicron particles generated by a four-beam optical lattice

Slama-Eliau

Raithel

2011

Phys. Rev. E

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Using an optical lattice formed by four laser beams, we obtain three-dimensional light-induced crystals of 490 nm diameter polystyrene spheres in solution. The setup yields face-centered orthorhombic optical crystals of a packing density of about 40%. An alignment procedure is developed in which the crystals are first prepared near a sample wall, and then in the bulk of the sample. A series of tests is performed that demonstrate particle trapping in all three dimensions.For one case, the trapping force is measured, and good agreement with a simple theoretical model is found. Possible applications are discussed.

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“…Other methods of particle translations, not directly applicable to our setup, include ones that are based on holographic techniques (for a recent, sophisticated method see Ref. [21]. )…”

Section: Methodsmentioning

confidence: 99%

Three-dimensional arrays of submicron particles generated by a four-beam optical lattice

Slama-Eliau

Raithel

2011

Phys. Rev. E

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“…This placed further limits on the maximum flow rate as the optical trapping force must be greater than the fluid's drag force acting on the particle. Future improvements could entail computer recognition of target cells, automated stage translation such that the optical trap intercepts the flowing cell [18], or the inclusion of beam shaping techniques and advanced user interfaces [26][27][28][29]. In addition, a combination of Raman spectra technique and optical tweezers could be also used to refine out method [30].…”

Section: Resultsmentioning

confidence: 99%

Optical sorting and cultivation of denitrifying anaerobic methane oxidation archaea

Carberry

Cai

et al. 2017

Biomed. Opt. Express

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Denitrifying anaerobic methane oxidizing (DAMO) microorganisms play an important role in the global carbon and nitrogen cycles as they are able to mediate methane oxidation using nitrite/nitrate under anoxic conditions. However, the physiological properties of DAMO microorganisms remain poorly understood, partially since the organisms are difficult to isolate or cultivate in pure culture and partially because of their long cultivation time. In this study, DAMO cell sorting has been conducted by integrating optical tweezers within enclosed microfluidic chips. This integrated cell sorting method has high purity, low infection rates, and causes no discernable harm to cell viability. The purity of the sorted cells was controlled by the microfluidic chip structure design and operation, while the cell viability was verified by imaging the cultured DAMO archaea after 420 days.

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“…It allows the multimodal study of physiological responses of single neurons and neural networks during optical operation (Grieve et al 2009), monitored by complementary types of electrophysiology and imaging techniques.…”

Section: Discussionmentioning

confidence: 99%

Integration of Optical Manipulation and Electrophysiological Tools to Modulate and Record Activity in Neural Networks

Difato

Schibalsky

Benfenati

et al. 2011

International Journal of Optomechatronics

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We present an optical system that combines IR (1064 nm) holographic optical tweezers with a sub-nanosecond-pulsed UV (355 nm) laser microdissector for the optical manipulation of single neurons and entire networks both on transparent and non-transparent substrates in vitro. The phase-modulated laser beam can illuminate the sample concurrently or independently from above or below assuring compatibility with different types of microelectrode array and patch-clamp electrophysiology. By combining electrophysiological and optical tools, neural activity in response to localized stimuli or injury can be studied and quantified at sub-cellular, cellular, and network level.

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Hands-on with optical tweezers: a multitouch interface for holographic optical trapping

Cited by 53 publications

References 17 publications

Three-dimensional arrays of submicron particles generated by a four-beam optical lattice

Three-dimensional arrays of submicron particles generated by a four-beam optical lattice

Optical sorting and cultivation of denitrifying anaerobic methane oxidation archaea

Integration of Optical Manipulation and Electrophysiological Tools to Modulate and Record Activity in Neural Networks

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