Hands-free sample preparation platform for nucleic acid analysis

Baier, Tobias; Hansen‐Hagge, Thomas E.; Gransee, Rainer; Crombé, A.; Schmahl, S.; Paulus, Caroline; Drese, Klaus; Keegan, Helen; Martín, C; O’Leary, John; Furuberg, Liv; Solli, Lars; Grønn, Petter; Falang, I. M.; Karlgård, Aina; Gulliksen, Anja; Karlsen, Frank

doi:10.1039/b910421f

Cited by 23 publications

(30 citation statements)

References 20 publications

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“…This technology has already been adapted for performing hands-free Nucleic Acid Diagnostic (NAD) (Gulliksen et al, 2005), including complete extraction of RNA and DNA (Baier et al, 2009). The disposable chips are designed to include all reagents needed for complete NAD, including the detection of different mRNA targets, and may be mass produced to a low cost adapted to a low-resource market.…”

Section: Discussionmentioning

confidence: 99%

A comprehensive evaluation of the accuracy of cervical pre-cancer detection methods in a high-risk area in East Congo

et al. 2010

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BACKGROUND: Given the high burden of cervical cancer in low-income settings, there is a need for a convenient and affordable method for detecting and treating pre-cancerous lesions. METHODS: Samples for comparing the accuracy of cytology, virology and histology were collected. Identification of HPV E6/E7 mRNA was performed using PreTect HPV-Proofer. HPV DNA detection was performed by GP5 þ /6 þ PCR, followed by reverse line blot (RLB) for typing. RESULTS: A total of 343 women, aged 25 -60 years, attending gynaecological polyclinics in DR Congo were included for sample enrolment. The test positivity rate was conventional and liquid-based cytology (LBC) at cutoff ASCUS þ of 6.9 and 6.6%, respectively; PreTect HPV-Proofer of 7.3%; and consensus DNA PCR for 14 HR types of 18.5%. Sixteen cases of CIN2 þ lesions were identified. Of these, conventional cytology identified 66.7% with a specificity of 96.2%, LBC identified 73.3% with a specificity of 96.9%, all at cutoff ASCUS þ. HR-HPV DNA detected all CIN2 þ cases with a specificity of 85.9%, whereas PreTect HPV-Proofer gave a sensitivity of 81.3% and a specificity of 96.6%. CONCLUSION: Both HPV detection assays showed a higher sensitivity for CIN2 þ than did cytological methods. Detecting E6/E7 mRNA from only a subset of HR HPVs, as is the case with PreTect HPV-Proofer, resulted in a similar specificity to cytology and a significantly higher specificity than consensus HR HPV DNA (Po0.0001).

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Section: Discussionmentioning

confidence: 99%

A comprehensive evaluation of the accuracy of cervical pre-cancer detection methods in a high-risk area in East Congo

et al. 2010

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“…DNA extraction methods offer the potential to concentrate analytes in urine samples to reach the LOD in technologies described in Section II [95]. Thus, methods for processing urine samples and extracting nucleic acids from the specimen on microfluidic devices have been developed [46], [95].…”

Section: Future Perspectivementioning

confidence: 99%

“…6(a) and (b)] [46]. The samples were fixed in PreservCyt, a methanol based solution that can fixate epithelial cells at room temperature for at least one month.…”

Section: A Optical Detection Technologiesmentioning

confidence: 99%

Advances in Nanotechnology and Microfluidics for Human Papillomavirus Diagnostics

et al. 2015

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| Human papillomavirus (HPV) has been shown in many studies as a prerequisite for the development of cervical cancer, which is the second most common and predominant form of malignancy among women worldwide, with 270 000 deaths (80% of whom live in developing countries) and 500 000 new cases per year. Early diagnosis of cervical cancer allows patients to be fully treated. Therefore, there is high clinical utility of HPV diagnostics even with binary positive/ negative indication of the presence of HPV in patient samples.Although the Pap smear is widely used for cervical cancer screening, this method still suffers from low sensitivity and specificity. Thus, simple, rapid and inexpensive diagnostic methods are needed to detect the etiology of cervical cancer, i.e., HPV, especially high-risk oncogenic subtypes 16 and 18.Here, we review the existing assays and platforms employed for HPV diagnosis, and highlight recent advances in nanotechnology and microfluidics that potentially enable new approaches for HPV diagnosis.

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“…In some biochemical assays, it is desirable to analyze the content of cells such as intracellular proteins, organelles, chromosomes, RNA, and DNA. To this end, buffers containing chaotropic salts as cell lysing agents can be used, [ 149 ] alternatively to lysing cells using heated surfaces, [ 150 ] centrifugation, [ 151 ] or inductive heating. [ 152 ] …”

Section: Cell Sortingmentioning

confidence: 99%

Microfluidic Chips for Point‐of‐Care Immunodiagnostics

2011

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We might be at the turning point where research in microfluidics undertaken in academia and industrial research laboratories, and substantially sponsored by public grants, may provide a range of portable and networked diagnostic devices. In this Progress Report, an overview on microfluidic devices that may become the next generation of point-of-care (POC) diagnostics is provided. First, we describe gaps and opportunities in medical diagnostics and how microfluidics can address these gaps using the example of immunodiagnostics. Next, we conceptualize how different technologies are converging into working microfluidic POC diagnostics devices. Technologies are explained from the perspective of sample interaction with components of a device. Specifically, we detail materials, surface treatment, sample processing, microfluidic elements (such as valves, pumps, and mixers), receptors, and analytes in the light of various biosensing concepts. Finally, we discuss the integration of components into accurate and reliable devices.

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Hands-free sample preparation platform for nucleic acid analysis

Cited by 23 publications

References 20 publications

A comprehensive evaluation of the accuracy of cervical pre-cancer detection methods in a high-risk area in East Congo

A comprehensive evaluation of the accuracy of cervical pre-cancer detection methods in a high-risk area in East Congo

Advances in Nanotechnology and Microfluidics for Human Papillomavirus Diagnostics

Microfluidic Chips for Point‐of‐Care Immunodiagnostics

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