Handheld Point-of-Care System for Rapid Detection of SARS-CoV-2 Extracted RNA in under 20 min

Rodríguez-Manzano, Jesús; Malpartida-Cardenas, Kenny; Moser, Nicolas; Pennisi, Ivana; Cavuto, Matthew L.; Miglietta, Luca; Moniri, Ahmad; Penn, Rebecca; Satta, Giovanni; Randell, Paul; Davies, Frances; Bolt, Frances; Barclay, William; Holmes, Alison; Georgiou, Pantelis

doi:10.1021/acscentsci.0c01288

Cited by 114 publications

(97 citation statements)

References 55 publications

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“…The method depends on the detection of SARS-CoV 2 from an extracted RNA samples. The developed LAMP assay was tested on a real-time benchtop instrument (RT-qLAMP), showing a lower limit of detection of 10 RNA copies per reaction [207]. The results showed sensitivity and specificity of 91% and 100%, respectively, compared to RT-qPCR and average positive detection times of 15.45 ± 4.43 min ( Fig.…”

Section: Genetic-based Analysis: Pcr Rt-qpcr Lamp and Crisprmentioning

confidence: 98%

“…The results showed sensitivity and specificity of 91% and 100%, respectively, compared to RT-qPCR and average positive detection times of 15.45 ± 4.43 min ( Fig. 5) [207]. Another POC diagnostic test based on microfluidic platforms was reported to detect viruses using the rolling circle amplification (RCA) method [208].…”

Section: Genetic-based Analysis: Pcr Rt-qpcr Lamp and Crisprmentioning

confidence: 98%

“…A rapid POC diagnostic test (< 20 min) based on RT-LAMP was reported using semiconductor technology ( Fig. 5) [207]. The method depends on the detection of SARS-CoV 2 from an extracted RNA samples.…”

Section: Genetic-based Analysis: Pcr Rt-qpcr Lamp and Crisprmentioning

confidence: 99%

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Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

Abdelhamid

Badr

2021

Nanotechnol. Environ. Eng.

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A sensitive method for diagnosing coronavirus disease 2019 (COVID-19) is highly required to fight the current and future global health threats due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV 2). However, most of the current methods exhibited high false-negative rates, resulting in patient misdiagnosis and impeding early treatment. Nanoparticles show promising performance and great potential to serve as a platform for diagnosing viral infection in a short time and with high sensitivity. This review highlighted the potential of nanoparticles as platforms for the diagnosis of COVID-19. Nanoparticles such as gold nanoparticles, magnetic nanoparticles, and graphene (G) were applied to detect SARS-CoV 2. They have been used for molecular-based diagnosis methods and serological methods. Nanoparticles improved specificity and shorten the time required for the diagnosis. They may be implemented into small devices that facilitate the self-diagnosis at home or in places such as airports and shops. Nanoparticles-based methods can be used for the analysis of virus-contaminated samples from a patient, surface, and air. The advantages and challenges were discussed to introduce useful information for designing a sensitive, fast, and low-cost diagnostic method. This review aims to present a helpful survey for the lesson learned from handling this outbreak to prepare ourself for future pandemic.

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Section: Genetic-based Analysis: Pcr Rt-qpcr Lamp and Crisprmentioning

confidence: 98%

Section: Genetic-based Analysis: Pcr Rt-qpcr Lamp and Crisprmentioning

confidence: 98%

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Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

Abdelhamid

Badr

2021

Nanotechnol. Environ. Eng.

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“…The common thread among the studies was developing a more practical design of SPR-based virus detection systems with a certain level of sensitivity. The research focus in recent years on virus detection technologies has often been on sensitivity and speed, and for on-site detection technologies, whether the system can be handheld or wireless and employs reusable methods [82][83][84][85][86]. As for the research on virus detection technology using SPR, the major points required in the future are not high sensitivity and rapidity, but thin film formation and suitable construction systems to enhance the practicality and applicability.…”

Section: Current Application Of Spr For Virus Sensingmentioning

confidence: 99%

Surface Plasmon Resonance (SPR)- and Localized SPR (LSPR)-Based Virus Sensing Systems: Optical Vibration of Nano- and Micro-Metallic Materials for the Development of Next-Generation Virus Detection Technology

Takemura

2021

Biosensors

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The global damage that a widespread viral infection can cause is evident from the ongoing COVID-19 pandemic. The importance of virus detection to prevent the spread of viruses has been reaffirmed by the pandemic and the associated social and economic damage. Surface plasmon resonance (SPR) in microscale and localized SPR (LSPR) in nanoscale virus sensing systems are thought to be useful as next-generation detection methods. Many studies have been conducted on ultra-sensitive technologies, especially those based on signal amplification. In some cases, it has been reported that even a low viral load can be measured, indicating that the virus can be detected in patients even in the early stages of the viral infection. These findings corroborate that SPR and LSPR are effective in minimizing false-positives and false-negatives that are prevalent in the existing virus detection techniques. In this review, the methods and signal responses of SPR and LSPR-based virus detection technologies are summarized. Furthermore, this review surveys some of the recent developments reported and discusses the limitations of SPR and LSPR-based virus detection as the next-generation detection technologies.

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“… 2 − 4 Though laboratory-based reverse transcriptase-polymerase chain reaction tests remain the gold standard for confirmation of SARS CoV-2 infestation, many molecular diagnostic methods including reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) assays have been reported. 5 , 6 However, we strongly believe that field deployment of these RT-LAMP assays is challenged by the high incidence of false positives, just like in the case of other LAMP assays explored for viral detections. 7 …”

Section: Introductionmentioning

confidence: 99%

Functionalized Co₂FeAl Nanoparticles for Detection of SARS CoV-2 Based on Reverse Transcriptase Loop-Mediated Isothermal Amplification

Dahiya

Gupt

Dhaka

et al. 2021

ACS Appl. Nano Mater.

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Loop-mediated isothermal amplification (LAMP) is a sensitive, efficient, and rapid nucleic acid amplification technique resulting in a large number of amplicons; however, it suffers from a high incidence of false positives due to carry-over and aerosol. Herein, we report a 10 min nano-capture system that is used in conjunction with a modified reverse transcriptase-LAMP (RT-LAMP) assay for the accurate detection of SARS CoV-2 virus. The nano-capture system employs in-house-designed probe-functionalized magnetic nanoparticles Co 2 FeAl (cobalt-based Heusler alloy) for efficient capture of contaminating amplicons from the reaction mixture preceding RT-LAMP. The nano-cleaned RT-LAMP assay along with engineered primers successfully detected the presence of 10 copies of SARS CoV-2 virus while completely eliminating the incidence of false positives. The presented contaminant-capture method has been compared with other approaches for elimination of contaminants and was found to be more effective. The insight brought in this work is the design of a rapid nano-capture system that hybridizes with contaminating amplicons (carry-over) with high specificity to enable easy removal from the assay for elimination of false positives. The method has been proven to be successful for RT-LAMP assays in the rapid and highly specific detection of SARS CoV-2, which is currently a major challenge for global health. To the best of our knowledge, this is the first work involving a nano-based cleaning strategy for reliable and rapid diagnosis using isothermal amplification approaches.

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Handheld Point-of-Care System for Rapid Detection of SARS-CoV-2 Extracted RNA in under 20 min

Cited by 114 publications

References 55 publications

Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

Surface Plasmon Resonance (SPR)- and Localized SPR (LSPR)-Based Virus Sensing Systems: Optical Vibration of Nano- and Micro-Metallic Materials for the Development of Next-Generation Virus Detection Technology

Functionalized Co₂FeAl Nanoparticles for Detection of SARS CoV-2 Based on Reverse Transcriptase Loop-Mediated Isothermal Amplification

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Handheld Point-of-Care System for Rapid Detection of SARS-CoV-2 Extracted RNA in under 20 min

Cited by 114 publications

References 55 publications

Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

Nanobiotechnology as a platform for the diagnosis of COVID-19: a review

Surface Plasmon Resonance (SPR)- and Localized SPR (LSPR)-Based Virus Sensing Systems: Optical Vibration of Nano- and Micro-Metallic Materials for the Development of Next-Generation Virus Detection Technology

Functionalized Co2FeAl Nanoparticles for Detection of SARS CoV-2 Based on Reverse Transcriptase Loop-Mediated Isothermal Amplification

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Product

Resources

About

Functionalized Co₂FeAl Nanoparticles for Detection of SARS CoV-2 Based on Reverse Transcriptase Loop-Mediated Isothermal Amplification