Handheld optical-resolution photoacoustic microscopy

Lin, Li; Zhang, Pengfei; Xu, Song; Shi, Junhui; Li, Lei; Yao, Junjie; Wang, Lidai; Zou, Jun

doi:10.1117/1.jbo.22.4.041002

Cited by 69 publications

(63 citation statements)

References 25 publications

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“…The penetration depth of such systems is, however, fundamentally limited by light scattering and, therefore, similar to that of RCM or MPM. To the best of our knowledge, microscopic optoacoustic devices have not yet entered clinical research . Optoacoustic mesoscopy methods use non‐focused illumination schemes in combination with a focused ultrasound detector, which enables imaging to depths of several millimetres while keeping high resolution .…”

Section: Dermatologic Imaging Using Raster‐scan Optoacoustic Mesoscopymentioning

confidence: 99%

“…To the best of our knowledge, microscopic optoacoustic devices have not yet entered clinical research. 46,[50][51][52][53] Optoacoustic mesoscopy methods use non-focused illumination schemes in combination with a focused ultrasound detector, which enables imaging to depths of several millimetres while keeping high resolution. [54][55][56][57][58] A fundamental limitation of previous systems has, however, been the narrow bandwidth of the applied ultrasound detectors.…”

Section: Light Absorptionmentioning

confidence: 99%

See 1 more Smart Citation

Non‐invasive imaging in dermatology and the unique potential of raster‐scan optoacoustic mesoscopy

Hindelang

Aguirre

Schwarz

et al. 2019

Acad Dermatol Venereol

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In recent years, several non‐invasive imaging methods have been introduced to facilitate diagnostics and therapy monitoring in dermatology. The microscopic imaging methods are restricted in their penetration depth, while the mesoscopic methods probe deeper but provide only morphological, not functional, information. ‘Raster‐scan optoacoustic mesoscopy’ ( RSOM ), an emerging new imaging technique, combines deep penetration with contrast based on light absorption, which provides morphological, molecular and functional information. Here, we compare the capabilities and limitations of currently available dermatological imaging methods and highlight the principles and unique abilities of RSOM . We illustrate the clinical potential of RSOM , in particular for non‐invasive diagnosis and monitoring of inflammatory and oncological skin diseases.

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Section: Dermatologic Imaging Using Raster‐scan Optoacoustic Mesoscopymentioning

confidence: 99%

Section: Light Absorptionmentioning

confidence: 99%

Non‐invasive imaging in dermatology and the unique potential of raster‐scan optoacoustic mesoscopy

Hindelang

Aguirre

Schwarz

et al. 2019

Acad Dermatol Venereol

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“…Optical resolution photoacoustic microscopy (ORPAM), featuring high spatial resolution, deep penetration depth and large FOV, is a noninvasive and label‐free imaging approach capable of monitoring brain hemodynamics at microscale level . Current developments of ORPAM mainly focus on miniaturization of the device using new scanning mechanisms and optical/acoustic scanners . However, it is challenging to develop a wearable photoacoustic microscope, which enables the investigation of brain activities on a freely moving rodent.…”

Section: Introductionmentioning

confidence: 99%

Wearable optical resolution photoacoustic microscopy

Chen

Xie

2019

Journal of Biophotonics

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Optical resolution photoacoustic microscopy (ORPAM) is an emerging imaging technique, which has been extensively used to study various brain activities and disorders of the anesthetized/restricted rodents with a special focus on the morphological and functional visualization of cerebral cortex. However, it is challenging to develop a wearable photoacoustic microscope, which enables the investigation of brain activities/disorders on freely moving rodents. Here, we report a wearable and robust optical resolution photoacoustic microscope (W-ORPAM), which utilizes a small, light, stable and fast optical scanner. This wearable imaging probe features high spatiotemporal resolution, large field of view (FOV) and easy assembly as well as adjustable optical focus during the in vivo experiment, which makes it accessible to image cerebral cortex activities of freely moving rodents. To demonstrate the advantages of this technique, we used W-ORPAM to monitor both morphological and functional variations of vasculature in cerebral cortex during the induction of ischemia and reperfusion of a freely moving rat.

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“…Exogenous contrast agents have also been helpful during the development of PAI imaging systems . Based on the imaging configuration, PAI systems are categorized into two groups: photoacoustic tomography and photoacoustic microscopy (PAM) . PAM is separated into two categories: acoustic‐resolution PAM (AR‐PAM) and optical‐resolution PAM (OR‐PAM).…”

Section: Introductionmentioning

confidence: 99%

Enhanced contrast acoustic‐resolution photoacoustic microscopy using double‐stage delay‐multiply‐and‐sum beamformer for vasculature imaging

Mozaffarzadeh

Varnosfaderani

Sharma

et al. 2019

Journal of Biophotonics

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In acoustic-resolution photoacoustic microscopy (AR-PAM) systems, the lateral resolution in the focal zone of the ultrasound (US) transducer is determined by the numerical aperture (NA) of the transducer. To have a high lateral resolution, a large NA is used. However, the larger the NA, the smaller the depth of focus [DOF]. As a result, the lateral resolution is deteriorated at depths out of the focal region. The synthetic aperture focusing technique (SAFT) along with a beamformer can be used to improve the resolution outside the focal region. In this work, for image formation in AR-PAM, we propose the double-stage delay-multiply-and-sum (DS_DMAS) algorithm to be combined with SAFT. The proposed method is evaluated experimentally using hair targets and in vivo vasculature imaging. It is shown that DS_DMAS provides a higher resolution and contrast compared to other methods. For the B-mode images obtained using the hair phantom, the proposed method reduces the average noise level for all the depths by about 134%, 57% and 23%, compared to the original low-resolution, SAFT+DAS and SAFT+DMAS methods, respectively. All the results indicate that the proposed method can be an appropriate algorithm for image formation in AR-PAM systems. K E Y W O R D S acoustic-resolution photoacoustic microscopy, contrast enhancement, synthetic aperture focusing technique, vasculature imaging, virtual source Moein Mozaffarzadeh, Mehdi H. H. Varnosfaderan and Arunima Sharma contributed equally to this study.

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Handheld optical-resolution photoacoustic microscopy

Cited by 69 publications

References 25 publications

Non‐invasive imaging in dermatology and the unique potential of raster‐scan optoacoustic mesoscopy

Non‐invasive imaging in dermatology and the unique potential of raster‐scan optoacoustic mesoscopy

Wearable optical resolution photoacoustic microscopy

Enhanced contrast acoustic‐resolution photoacoustic microscopy using double‐stage delay‐multiply‐and‐sum beamformer for vasculature imaging

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